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Bacillus subtilis Collection
version 1
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
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iGEM Parts Registry
version 1
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
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Devices from the iGEM 2016 interlab
version 1
This is a collection of devices that were used in the 2016 iGEM interlab study
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Intein_assisted_Bisection_Mapping
version 1
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
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SBOL Compliant Software
version 1
A collection of software that supports the Synthetic Biology Open Language (SBOL) standard
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SynBioHub
SynBioHub is a design repository for people designing biological constructs. It enables DNA and protein designs to be uploaded, then provides a shareable link to allow others to view them. SynBioHub also facilitates searching for information about existing useful parts and designs by combining data from a variety of sources.
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LCP SynBioHub
SynBioHub is a design repository for people designing biological constructs. It enables DNA and protein designs to be uploaded, then provides a shareable link to allow others to view them. SynBioHub also facilitates searching for information about existing useful parts and designs by combining data from a variety of sources.
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SEVAhub
The SEVA database (SEVA-DB) is a resource for implementation of a standard for physical assembly of vector plasmids and for their non-ambiguous nomenclature as well as the index for a repository of functional sequences and actual constructs available to the community. This resource, SEVAhub, is an instance of the SynBioHub design repository providing access to SEVA-DB converted to the Synthetic Biology Open Language (SBOL).