Cryptococcus neoformans Database in Synthetic Biology Open Language
type: Collection
persistent identity: CnD collection
display id: CnD_collection
version: 1
description: This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
OBI_0001617: 10352226
OBI_0001617: 11270405
OBI_0001617: 14523045
OBI_0001617: 18952189
OBI_0001617: 2201894
OBI_0001617: 23460208
OBI_0001617: 25417972
OBI_0001617: 27503169
OBI_0001617: 27711143
OBI_0001617: 27780958
OBI_0001617: 31220607
OBI_0001617: 8531025
OBI_0001617: 8577246
OBI_0001617: 8971627
creator: Sophia Garcia de Resende
created: 2022-06-09T18:01:02.159Z
created: 2022-06-09T18:03:03.460Z
created: 2022-06-10T14:30:46.032Z
created: 2022-06-10T14:40:00.663Z
created: 2022-06-10T15:00:35.912Z
created: 2022-06-10T15:14:43.155Z
created: 2022-06-10T15:25:58.350Z
created: 2022-06-10T19:46:22.229Z
created: 2022-06-10T19:54:23.232Z
created: 2022-06-10T20:00:14.259Z
created: 2022-06-10T20:03:14.702Z
created: 2022-06-10T20:17:28.569Z
created: 2022-06-10T20:20:40.774Z
created: 2022-06-10T20:44:42.029Z
created: 2022-06-10T20:49:40.490Z
created: 2022-06-10T21:03:11.839Z
created: 2022-06-10T21:09:04.989Z
created: 2022-06-10T21:18:53.773Z
created: 2022-06-10T21:25:19.468Z
created: 2022-06-10T21:36:47.235Z
created: 2022-06-10T21:39:08.244Z
created: 2022-06-10T21:43:20.810Z
created: 2022-06-10T21:46:26.909Z
created: 2022-06-10T21:49:02.149Z
created: 2022-06-20T00:03:31.385Z
modified: 2022-06-22T20:46:13
mutable description: This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
mutable provenance: The parts were obtain through a extensive literature review and by search of plasmids in databases such as NCBI and AddGene.
owned by: sophia
member: https://synbiohub.org/public/CnDatabase/Hygromicin_ResistanceSequence/1
member: TEF1
member: https://synbiohub.org/public/CnDatabase/GenBank_CPSequence/1
member: GPD
member: https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/1
member: UGE2
member: https://synbiohub.org/public/CnDatabase/mCherrySequence/1
member: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/1
member: APP1
member: https://synbiohub.org/public/CnDatabase/Neomicin_ResistanceSequence/1
member: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540Sequence/1
member: GAL7
member: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263Sequence/1
member: https://synbiohub.org/public/CnDatabase/GenBank_AE017353_Region_190704_1911746_SBOLDesignerActivity/1
member: ACT1
member: https://synbiohub.org/public/CnDatabase/GenBank_AE017346_Region_940695_942557_SBOLDesignerActivity/1
member: mCherry
member: https://synbiohub.org/public/CnDatabase/CnU6Sequence/1
member: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/1
member: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_375181_376053Sequence/1
member: NEO (G418/Neomicin Resistance)
member: URA5
member: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176110_177085Sequence/1
member: amdS
member: SBOLDesigner CAD Tool
member: https://synbiohub.org/public/CnDatabase/GenBank_CP003830_Region_176667_176966_SBOLDesignerActivity/1
member: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistanceSequence/1
member: GFP
member: https://synbiohub.org/public/CnDatabase/GenBank_CP003828_Region_1023919_1024166_SBOLDesignerActivity/1
member: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/1

HOG1
type: ComponentDefinition
persistent identity: GenBank CP003830 Region 176667 176966
display id: GenBank_CP003830_Region_176667_176966
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003830_Region_176667_176966_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubi H99
OBI_0001617: 27780958
modified: 2022-06-20T00:54:38
mutable description: This is the terminator of mitogen-activated protein kinase (HOG1).
mutable notes: This terminator is delimited by primers present in the reference article. There is a SNP in relation to the genomic sequence (CP003830). We used the genomic sequence.
mutable provenance: GenBank: CP003830 Region: 176667..176966 Plasmid: YSBE 508 (#92081)
owned by: sophia
type: DnaRegion
role: SO:0000951
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003830_Region_176667_176966Sequence/1

GPD
type: ComponentDefinition
persistent identity: GenBank AE017346 Region 940695 942557
display id: GenBank_AE017346_Region_940695_942557
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_AE017346_Region_940695_942557_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans JEC21
OBI_0001617: 25417972
modified: 2022-06-18T14:57:15
mutable description: This is the promoter of glyceraldehyde-3-phosphate dehydrogenase.
mutable notes: This sequence was directly sent personally by Dr. Chen Ding. We did a BLAST and found a deletion of five nucleotides in comparison with the Cryptococcus neoformans var. neoformans JEC21 chromosome sequence. Thus, we used the genomic sequence (AE017346).
mutable provenance: GenBank: AE017346 Region: 940695..942557
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_AE017346_Region_940695_942557Sequence/1

ACT1
type: ComponentDefinition
persistent identity: GenBank CP003820 Region 1242235 1243067
display id: GenBank_CP003820_Region_1242235_1243067
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
OBI_0001617: 27711143
modified: 2022-06-21T02:04:04
mutable description: This is actin's promoter.
mutable notes: The plasmid pSDMA66 (#128355) sequence has an A in position 84, and the chromosome sequence has a G. We used the last one.
mutable provenance: Plasmid pSDMA66 (#12835) GenBank: CP003820 Region: 1242235..12430676
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067Sequence/1

amdS
type: ComponentDefinition
persistent identity: amdS
display id: amdS
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/amdS_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans serotype A strain H99O
OBI_0001617: 31220607
modified: 2022-06-20T16:20:10
mutable description: This is the acetamidase coding sequence.
mutable notes: amdS as a selective marker for C. neoformans.
mutable provenance: Plasmid pPEE7 (#128357)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/amdSSequence/1

CnU6
type: ComponentDefinition
persistent identity: CnU6 terminator
display id: CnU6_terminator
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/CnU6_terminator_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans JEC21
OBI_0001617: 27503169
modified: 2022-06-10T21:37:57
mutable description: This is the U6 terminator.
mutable notes: Specific to RNApol lII. Available on Reference Article's Supplementary Material. We couldn't find the correspondent GenBank ID because this sequence was too small.
mutable provenance: Available on the Supplementary Material of WANG, et al. 2016.
owned by: sophia
type: DnaRegion
role: SO:0000951
sequence: https://synbiohub.org/public/CnDatabase/CnU6Sequence/1

UGE2
type: ComponentDefinition
persistent identity: GenBank CP003831 Region 176453 177430
display id: GenBank_CP003831_Region_176453_177430
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubii H99
OBI_0001617: 18952189
modified: 2022-06-10T20:01:17
mutable description: This is the promoter of the UDP-glucose epimerase.
mutable notes: This promoter UGE2 is repressible by glucose and inducible by galactose. The 3' primer used to amplify the UGE2 promoter region encopassed the first codons of UGE2 protein. This region was, therefore, excluded from the promoter presented here, diminishing the size of this promoter from 1007 bp to 978 bp.
mutable provenance: GenBank: CP003831 Region: 176453..177430
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430Sequence/1

NAT (Nourseothricin Resistance/NrsR)
type: ComponentDefinition
persistent identity: Nourseothricin resistance
display id: Nourseothricin_resistance
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans serotype A H99S strain
OBI_0001617: 11270405
OBI_0001617: 27780958
modified: 2022-06-20T00:13:47
mutable description: This is the coding sequence of the Nourseothricin Resistance gene (NAT/NrsR).
mutable notes: We deleted the restriction enzyme sequence of NotI and added the atg codon the beginning of the sequence.
mutable provenance: Plasmid YSBE 557 (#92085)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistanceSequence/1

GAL7
type: ComponentDefinition
persistent identity: GenBank CP003831 Region 178967 179986
display id: GenBank_CP003831_Region_178967_179986
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_178967_179986_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubii H99
OBI_0001617: 18952189
modified: 2022-06-22T19:42:10
mutable description: This is the galactose transferase promoter.
mutable notes: This promoter, GAL7 + 5'UTR, is repressible by glucose and inducible by galactose. The 3' primer used to amplify the GAL7 promoter region encopassed the first codon ATG. This ATG codon was, therefore, excluded from the promoter presented here, diminishing the size of this promoter from 1022 to 1020 bp.
mutable provenance: GenBank: CP003831 Region: 178967..179986
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_178967_179986Sequence/1

TEF1
type: ComponentDefinition
persistent identity: GenBank CP003831 Region 375181 376053
display id: GenBank_CP003831_Region_375181_376053
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_375181_376053_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubi H99
OBI_0001617: 27711143
modified: 2022-06-20T00:53:32
mutable description: This is the terminator of the Elongation Factor 1.
mutable notes: TEF1 terminator has 100% identity to the Cryptococcus neoformans genomic sequence (CP003831).
mutable provenance: GenBank: CP003831 Region: 375181..376053 Plasmid: pSDMA65 (#128354)
owned by: sophia
type: DnaRegion
role: SO:0000951
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_375181_376053Sequence/1

mCherry
type: ComponentDefinition
persistent identity: mCherry
display id: mCherry
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/mCherry_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans serotype A H99S strain
OBI_0001617: 27780958
modified: 2022-06-18T16:38:59
mutable description: This is the coding sequence of the Fluorescent Protein mCherry.
mutable notes: The atg codon was added at the beginning of the sequence. The authors reported at the Addgene website that: “Addgene's sequencing results found K128R and Q193P mutations in mCherry. These mutations are not known to affect plasmid function”.
mutable provenance: Plasmid YSBE 596 (#92088)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/mCherrySequence/1

NEO (G418/Neomicin Resistance)
type: ComponentDefinition
persistent identity: Neomicin Resistance
display id: Neomicin_Resistance
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Neomicin_Resistance_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans serotype A strain H99O
OBI_0001617: 10618292
OBI_0001617: 31220607
modified: 2022-06-20T00:19:31
mutable description: This is the coding sequence of the Neomycin Resistance gene (NEO/G418).
mutable notes: Present in plasmid pPEE33.
mutable provenance: Plasmid pPEE33 (#128373)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/Neomicin_ResistanceSequence/1

URA5
type: ComponentDefinition
persistent identity: GenBank CM000046 Region 302227 303005
display id: GenBank_CM000046_Region_302227_303005
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_302227_303005_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans B-3501A
OBI_0001617: 2201894
modified: 2022-06-20T00:29:43
mutable description: This is the coding sequence of the Orotidine Monophosphate Pyrophosphorylase.
mutable notes: This genomic sequence (CM000046) is the same sequence described in the reference article (M34606).
mutable provenance: Genbank: CM000046 Region: 302227..303005 and M34606 Region: 714..1492
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_302227_303005Sequence/1

Cas9
type: ComponentDefinition
persistent identity: Cas9
display id: Cas9
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubi H99
OBI_0001617: 23460208
OBI_0001617: 27711143
modified: 2022-06-18T16:22:24
mutable description: This is the coding sequence of CRISPR associated protein 9 from the widely applied CRISPR-Cas system gene editing tool.
mutable notes: Originally, the Cas9 nucleotide sequence was PCR amplified from the Addgene plasmid #43802, and it has 2 SNPs in relation to the original one, but both sequences code the same aminoacid. At the C- terminal end of Cas9, there is the Nuclear Localization Signal (NLS), SV40 NLS.
mutable provenance: Plasmid pSDMA65 (#128354) Plasmid p414-TEF1p-Cas9-CYC1t (#43802)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/Cas9Sequence/1

URA5
type: ComponentDefinition
persistent identity: GenBank CM000046 Region 301512 302226
display id: GenBank_CM000046_Region_301512_302226
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_301512_302226_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans B-3501A
OBI_0001617: 2201894
modified: 2022-06-18T16:06:43
mutable description: This is the promoter of the Orotidine Monophosphate Pyrophosphorylase.
mutable notes: We did a BLAST with the article's sequence (M34606) and we found two deletions. Thus, the sequence provided here is the updated one, the genomic sequence (CM000046), which has 2 more bp.
mutable provenance: GenBank: CM000046 Region: 301512..302226
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_301512_302226Sequence/1

TEF1
type: ComponentDefinition
persistent identity: GenBank CP003831 Region 377755 378976
display id: GenBank_CP003831_Region_377755_378976
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubi H99
OBI_0001617: 27711143
modified: 2022-06-22T19:51:51
mutable description: This is the promoter of the Elongation Factor 1.
mutable notes: The plasmid TEF1 sequence (#128354) has an SNP in position 168 compared to the genomic sequence (CP003831). We used the genomic sequence.
mutable provenance: Plasmid pSDMA65 (#128354) Genbank: CP003831 Region: 377755..378976
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976Sequence/1

CnU6
type: ComponentDefinition
persistent identity: GenBank AE017348 Region 871991 872263
display id: GenBank_AE017348_Region_871991_872263
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans JEC21
OBI_0001617: 27503169
modified: 2022-06-18T14:32:19
mutable description: This is the U6 promoter.
mutable notes: That is an endogenous promoter of C. neoformans serotype D strain JEC21. The sequence is available on Reference Article's Supplementary Material and it presented 100% identity with C.neoformans genomic sequence (AE017348).
mutable provenance: GenBank: AE017348.1 Region: 871991..872263
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263Sequence/1

URA5
type: ComponentDefinition
persistent identity: GenBank CM000046 Region 303006 303540
display id: GenBank_CM000046_Region_303006_303540
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans B-3501A
OBI_0001617: 2201894
modified: 2022-06-20T01:04:05
mutable description: This is the terminator of the Orotidine Monophosphate Pyrophosphorylase.
mutable notes: The Ura5 terminator (M34606) given by the reference has some SNPs compared to the genomic sequence (CM000046) present in this database.
mutable provenance: GenBank: CM000046 Region: 303006..303540
owned by: sophia
type: DnaRegion
role: SO:0000951
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540Sequence/1

GFP
type: ComponentDefinition
persistent identity: GFP
display id: GFP
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GFP_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans serotype A H99S
OBI_0001617: 27780958
modified: 2022-06-10T23:49:42
mutable description: This is the coding sequence of the Green Fluorescent Protein (GFP).
mutable notes: The atg codon was added at the beginning of the sequence. Present in plasmids: YSBE 508, YSBE 557 e YSBE 608 of the same article.
mutable provenance: Plasmid YSBE 557 (#92085) Plasmid YSBE 608 (#92089) Plasmid YSBE 508 (#92081)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/GFPSequence/1

APP1
type: ComponentDefinition
persistent identity: GenBank CP047908 Region 1244167 1244926
display id: GenBank_CP047908_Region_1244167_1244926
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
OBI_0001617: 14523045
modified: 2022-06-18T01:05:23
mutable description: This is the promoter of antiphagocytic protein.
mutable notes: In this article they renamed the previously isolated IP1 to antiphagocytic protein 1 (App1). The chromosome sequence (CP047908) has some SNP's and gaps compared to the article sequence (AF180108). We used the chromosome sequence.
mutable provenance: GenBank: CP047908 Region: 1244167..1244926
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CPSequence/1

HYG
type: ComponentDefinition
persistent identity: Hygromicin Resistance
display id: Hygromicin_Resistance
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Hygromicin_Resistance_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans serotype A strain H99O
OBI_0001617: 31220607
OBI_0001617: 8971627
modified: 2022-06-10T20:53:49
mutable description: This is the coding sequence of the Hygromycin Resistance gene.
mutable notes: Present in plasmid pPEE38.
mutable provenance: Plasmid pPEE38 (#128378)
owned by: sophia
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/CnDatabase/Hygromicin_ResistanceSequence/1

GAL7
type: ComponentDefinition
persistent identity: GenBank AE017353 Region 190704 1911746
display id: GenBank_AE017353_Region_190704_1911746
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_AE017353_Region_190704_1911746_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. neoformans JEC21
OBI_0001617: 8577246
modified: 2022-06-18T14:49:47
mutable description: This is the galactose-1-fosfato uridililtransferase promoter.
mutable notes: The GAL7 promoter is induced by galactose and repressed by glucose. The 3' primer used to amplify the GAL7 promoter region encopassed part of the first exon, therefore this part was excluded from the promoter presented here, diminishing the size of this promoter from 582 to 471 bp. Some SNPs were also detected in relation to the sequence presented in the original article, therefore the genomic sequence (AE017353) was used here.
mutable provenance: GenBank: AE017353 Region: 190704..191174
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_AE017353_Region_190704_1911746Sequence/1

GAL1
type: ComponentDefinition
persistent identity: GenBank CP003831 Region 176110 177085
display id: GenBank_CP003831_Region_176110_177085
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176110_177085_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubii H99
OBI_0001617: 18952189
modified: 2022-06-18T14:37:57
mutable description: This is the galactose kinase promoter.
mutable notes: This promoter, GAL1 + 5'UTR, is repressible by glucose and inducible by galactose. The 3' primer used to amplify the GAL1 promoter region encompassed the first codon ATG. This ATG codon was, therefore, excluded from the promoter presented here, diminishing the size of this promoter from 978 to 976 bp.
mutable provenance: Genbank: CP003831 Region: 176110..177085
owned by: sophia
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176110_177085Sequence/1

TRP1
type: ComponentDefinition
persistent identity: GenBank CP003828 Region 1023919 1024166
display id: GenBank_CP003828_Region_1023919_1024166
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003828_Region_1023919_1024166_SBOLDesignerActivity/1
description: Organism: Cryptococcus neoformans var. grubi H99
OBI_0001617: 31220607
modified: 2022-06-20T01:01:36
mutable description: Terminator sequence of Phosphoribosylanthranilate Isomerase.
mutable notes: We used the genomic sequence (CP003828), that has a SNP, when compared with the sequence of the plasmids presented in the reference.
mutable provenance: Plasmid pPEE33 (#128373) Plasmid pPEE38 (#128378) GenBank: CP003828 Region: 1023919..1024166
owned by: sophia
type: DnaRegion
role: SO:0000951
sequence: https://synbiohub.org/public/CnDatabase/GenBank_CP003828_Region_1023919_1024166Sequence/1

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/1
owned by: sophia
elements: gcggggagtacaggctaagcgtaggcataagcatcagtgtgatggtcagggacccttgacaatcctcacaaagagcgagtaatctatatgctcccctaacacattgactcttcccggtgctcgaacaatatagttgggttgcccgccaaactcttttacaaaagtttgaactagagcgctccacctttgcctgtgatgaacaaatacagtgtcagccggttgattctgttatatctgcgtaacaacgtacctctcccgctgaagagcttcctctgtgttataaatctcattgagattgtgaaatgcaggaacaggtcgtagctcctccggacccattttgaacatgatatacctgcggaaaacaagaccgtcgattcggctttgtgggcgatataaatagtggtttcattgtctggactaagtgggctgtctttccgatggcgctcgcaacgtgtcaacgtatacttgcaaaagttgctatgtacaatatcgaaagacatttacaaatcggcatgaaggtttgtgtggttcgctgtgaattgtccctgaataatgcacgcggacactctccggacaaacgatccggacttgagccctgctggcaagaaatagaaattattcccatttgatgtcatcatcgatgcaacagaattcccgtttatcattttctcttctacacattcccgttcgcagaattcccgttcatcgcttcctctactacacattcccgttcgcagaattgcccatcctcgaaattcccgttcgaacaaagcattatttgggcaagattcccgttcgtgctcgggtccaattctgagtattcccgttcatgattctgttgcatcgactttccctaataggtgttgggaagaggatgtcagcatccagtataaaagatgtagaatgcagcctcaatgaccatgagctatgatagcaaacattgaatcgcaataacacactcaaccgctcacctgcatt
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity/1
owned by: sophia
elements: gctgcgaggatgtgagctggagagcggcgcacgggaaggggaggactcacataagcatgcaggattcgagtggcatggtgtgcgctgagtgtatggttgtcggaggagaggatgatggtaacaacaatagcagcaacgtcactcgacgcgcgtccggtgtgccacacggggtaacgccgagtcgccgtcagggtcgccgagaccactctcacagcgtcaccgttggcaccagctcagcttacagcttctatcctccgccagcatccacatacatcccctataccgcatcccccacccactgcccaaggtgagtcatcttcccgcccccttcccttgcccgccactcagtcctccatcctccactaatccaccttatcgcacccaccgcctatcgcacatccgagcacaatgctgggcctgccaggggctgctagatggtgctctccccacgctgatctgcatgccggccattggatcatgggtgctaggtgctgggtgctggatgttggatgctggatgctgggtgcacgcttggtcatttccttccaggattgacggtcgccgagaggacgacgtggcgttcgacaacgagggccgatagcaccgcatcgcctcgacctgcatccatctcgccttgtccttttggtgcaacaatccatccgtgctggtgccacacgcatagctggaagagatggatgtgcgttgaacagagctgccgtcaggactttttggtgcacggaccctattgtcctccccaatcttcaccgcgtctcctaatatgcagcctctttgctaattgtctttttccattagtaaactcgcccaacatgtct
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/Cas9Sequence/1
type: Sequence
persistent identity: Cas9Sequence
display id: Cas9Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/1
owned by: sophia
elements: atggacaagaagtacagcattgggctcgatatcggcacaaacagcgtcggttgggccgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgatcgccacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggccgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgctacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttccataggctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctttggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaagaagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggcgcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacagcgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaacccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaagatgccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagtatgatcaagcgctatgatgagcaccaccaagacttgactttgctgaaggcccttgtcagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgttgcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaattccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcacagtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctgtctggagagcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagttaccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttgaaatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaacttacgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatgggggcggctgtcaagaaaactgat…
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_302227_303005Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_302227_303005_SBOLDesignerActivity/1
owned by: sophia
elements: atgtcctcccaagccctcgactccgccaaagttgccttcatcgaggctgccatcgaacatggcgtgcttcttttcggcaactttaccttgaagtccggccggtgagccatattgcagcgcttcacagtccaatcgaatctgacatgtgttcagccaatccccttacttcttcaatgccggtctcctttactcttcatcgcttctctcaactaccgctcaggcttacgccaaggtactttcctcttctaggattcctgactttgacgtcctcttcggcccagcttacaagggtatctccttggctgctgtctccgctgtaagcctttatcagcaaaccggcaaagatatcggctactgctacaacaggaaggagaagaaggacgtgagtctgtcctaaccagtgcgacagcgatgagctcataagccagtagcacggtgagggcggtactatggtcggtgcgcctctcaagggacgaatcgtcatcatcgacgatgttctcacctctggcaaggccatccgtgaagctattgacattctcaaggcctcccctgaagcgaagcttgtcggaattgtccagcttgtcgacagacaagagaaaggccagagcggtagcggcaagagtaccgtacaggaggttgaggaagagttcggtgtgcctgtcgagcctattattggtttggacgacattgtgaagtacttagaaagctccggcaagtgggaaaaggagctgcaagaggtcaggaagtacagggcggagtacggtgttcagaggtcttaa
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003828_Region_1023919_1024166Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003828_Region_1023919_1024166_SBOLDesignerActivity/1
owned by: sophia
elements: gtgaaggcggtaaggggttaattttccttagagggtgtatatatacatattagagaagtgatacaattttagcacactgcgaatccgagacagacatcgtgtcaatcatcttttttgacattatatgcccattaatctatctacagacaacaataccatccttcccaccctcagcaacgccgttgaatcctcaggatcttcatggctccttgtctctgaaaccaggaagctagtttctacatctcttc
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CPSequence/1
type: Sequence
persistent identity: GenBank CPSequence
display id: GenBank_CPSequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity/1
owned by: sophia
elements: ctttgggagatggcggctcactttgtaagaaaattcatgtaggtacaatacgcataccattgcttatttttggttgcatattgtcgtgctgttttcaatgttaaattacttttgcgaagagcagcgatatcttgcatcgtgtgagccgtgcgaggatcgagagtgtcagcaggttggaatgggtcaagccccttagtgcgtctggtagctggcattttgcaaggtgggggcagtcaaaaaaagttgaacgatggatatgagcaatgttgttcttagaacggaagtaccatggatgaggttgctgcaacgaatgacattttatgtgtggagctgacgccgagactaatgaggcggcatttccattacgaaagatactaacccgggacaggttgagcaaagcttcagtaaactgtagtttactggaactgatgtaacaggcaacaggtcgtgtaaattctagtttactggaacgctaccttgggacatttgaaaaaatcggcgacccccactatggggacatgtccccgcggctcgccttgtcccgggtccctggttccagagaacacctgcttgctgacttgctgctcttcttacagcgattactatgtagtcacctgtcatgacgtcaaaagtcgtactttctaatcgcgcgcgccatctggcatcttgtctcatgacctgttccagatataaaagcatcttttcccaggcattacattcttccgttgagttatctgtaagccaacccaacaaataatac
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/Nourseothricin_resistanceSequence/1
type: Sequence
persistent identity: Nourseothricin resistanceSequence
display id: Nourseothricin_resistanceSequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/1
owned by: sophia
elements: atgactcttgacgacacggcttaccggtaccgcaccagtgtcccgggggacgccgaggccatcgaggcactggatgggtccttcaccaccgacaccgtcttccgcgtcaccgccaccggggacggcttcaccctgcgggaggtgccggtggacccgcccctgaccaaggtgttccccgacgacgaatcggacgacgaatcggacgacggggaggacggcgacccggactcccggacgttcgtcgcgtacggggacgacggcgacctggcgggcttcgtggtcgtctcgtactccggctggaaccgccggctgaccgtcgaggacatcgaggtcgccccggagcaccgggggcacggggtcgggcgcgcgttgatggggctcgcgacggagttcgcccgcgagcggggcgccgggcacctctggctggaggtcaccaacgtcaacgcaccggcgatccacgcgtaccggcggatggggttcaccctctgcggcctggacaccgccctgtacgacggcaccgcctcggacggcgagcaggcgctctacatgagcatgccctgcccctaa
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_375181_376053Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_375181_376053_SBOLDesignerActivity/1
owned by: sophia
elements: ttacttatgtagtgacccttcttacgcgtaatatgcatcggatgactgttgctacttgtgcttcttacgttttgtgtcttatttgctgatgtatacctctgcgcttcaaaatcttcattgccgtgatatatcattccatgtcgtttgtccgttattggattcccgatagttgtccgcgaaatcgaatatttctcggctgacgacgtctcttgcaacaataaaatggccatcactgttgaaatttgcacgagagctataacttggaataaccttcgttgctgctttattttagtgctttcaactatttactcattctcaggtcgccaccatcgcattgatatcgttccttccatccttcctttcctttagtcggtcacgtcttccttaatccgtcactccttttttcagtcgttcctggagatcaccacgaaataatctcgacatgcactgaccatctcggttctgtggactgtcatgctaagagagttcatgttgtaataatagtttgttgcatggaaagaacggggaaagaagcaagaaacagatgtacagaattaccgatgtctctacgcatccctgacctcgtccttcctaccgcaaccgcacttccacctgctcacacgtcctcgttaatgccttgaatctcagccacacacttacctcgcctcgacgcttgtcgggccagggcacccccttcatcaacctgggaagagttggcggggcaaacggaggagggatggggcaggaagagggggatggcgcacgtcgagtggatttggaagaaaaggggttggtcgaggtggaagtcgaagcgaagatgttgaatgtcatgtttgggaacgggggatctttagaagactatgagattgaacg
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity/1
owned by: sophia
elements: gggttttcttcttaaatgcacgggtttaggtctagctaatcaagttccgacatattacaagtttgtaagcttgtatcaaaggaacttaagtacaggcaggcgtgctgaggcgacaaaggaagctgtaatatgattgttggctgtcaatcttcatcgtatctactttgtcaatactgacttcaatgacccaataatacaattttattagtgttgacccagaatggttagcaggaaactccccttctcttcctctcaatcccaatcatacttcatatctcctgctccccccatttccgtcttcctcgatgactccctggtcccatccctcccacctcctggaggcaagctggagcacctggacctaatgggtcgtcgccccaaattgcctccacctcttaagacaatcatcgtccaatcaaactctgaactatcttccaagccaatggccggatctggacacaagatgatgtcgaagccgcttgatgtgccagggttggtccgtggcctggagacacgtagagcgggtagtctggga
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003830_Region_176667_176966Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003830_Region_176667_176966_SBOLDesignerActivity/1
owned by: sophia
elements: aagaatcgttcttctttctttctttctttttatttataacgaaagaagagatgttggcgtatacttgaacttatgaatgatgtgttgtattcaggagcctgaaagtaatgtggtgtgtggtgggcggtgggacgaggaagagtcgggataagttgggatgatagatggtggagcaagattgcataagcaaaagttgagaagcgcaaagttgagaagaagtgtggacggctcgtgttgcgggttcagggagcagaagggagggaatgtggagattgttcggagagagggatagattgggac
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_AE017353_Region_190704_1911746Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_AE017353_Region_190704_1911746_SBOLDesignerActivity/1
owned by: sophia
elements: agaagcaggtcttgtcgaaccccggggatgcgacagcagaggttcgaacacgcaaaatcgtaatttgatatttatatgcgcgcccaccttcgctgacaattcccgttcatgaatcgtttgtcagtaattgacgcgccgggggtgccttgggggagtgttgtcaattaacatacaaatattcccgttcgtatcaatttgcatatccatctcatcacatagtagtattcccgttcgtatccatttgcatatctcgtcacatagtcttcccgttcgtatccatttcatatcgtaacatcatacagtcttcacaatcttcccgttcgtcctctcatcgtcaagccattgattgtcaatacacgcctacatattcccgttctcttgtctgccacatcgctcgtgcgttcttcccgtttttatgggaagactggggtcatgtcgtcaatacagcagcagacataatgggtgttatat
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_178967_179986Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_178967_179986_SBOLDesignerActivity/1
owned by: sophia
elements: gatcaggcgctggctgtgagttgattgccgctgttctgccttttcccccatccccgtcctgacgctctgatactgcagtctatccattgatggaggggagcaagatagtatagtgaagtcgtattgtctctgcaatgtaattctctttgccatgcatatgcttgagcctgtgccttctctttaatcgacagcactgacaatctcttgcagtttcgtcgcagtagtcacgcgtatatgtttccatagttccggcgggaagttatcttactaaatacggtatcgaaggaattacacaatatttgtactccggcggtggaaacaggtcttgtcgaaccctgatgaagcgaaagctaagaggttcgaaagtagcaaaatcatactttaaaatcttttatacgcgagtccaacttcgctgacaagaaaatggtagtgggccttgcgaatgaatgaggagagaaagttttagctgtggattcccagcaggcctccttacttaagtttgattaaaaattattacgaaaagatatatatagtaataaatttgaaatgaactaatcataatcgaaaaattcccgttcaacataaattcccgttcatttaaattcccgttcaacataaattcccgttcatttaaattcccgttcaacataaaattcccgttcataaattcccatggcattcatcatcatgaatcctttgttgtatagttattatataacggatattcccgttcgtatccattgcatatcttatcgcatagtcttcccgttcatcgtctcatcatcaagttattgattgtcaattcacgcttacatattcccgttcttctgtctaccatctatctatcacatcatctgtgaattcttcccgtttttatgggaagactgtcgtcatgttgtcaattgagcagcagacataatcgatgctatatatctagagcttcacggctcaggctgaacttggattaatgtggactactttcacaacgaatacttatcagcactcaattctctcctgaga
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176110_177085Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176110_177085_SBOLDesignerActivity/1
owned by: sophia
elements: ctattaaagatcgaagatcagctatgccggtccatgccatgtatatggacttaccctacttcattttgggctatttgattgcagcgctcgagagcttgagggtatgcgttgtgcgcattgtcaatgatgatgggcaagtagcgaccagagaccagaagtgagaggacaacgtgagatcctatgtatccgagtccaccggtgacaaggacgttctgtaatgagaattagccttggttttgttgtaattgtaatagaatggtcccactttcaaaggctgtccatggacagacaagcttgatgtcgacgtttggttcatggtaactcgagtctgttcaaaaaccataatgcaggtgagcggttgagtgtgttattgcgattcaatgtttgctatcatagctcatggtcattgaggctgcattctacatcttttatactggatgctgacatcctcttcccaacacctattagggaaagtcgatgcaacagaatcatgaacgggaatactcagaattggacccgagcacgaacgggaatcttgcccaaataatgctttgttcgaacgggaatttcgaggatgggcaattctgcgaacgggaatgtgtagtagaggaagcgatgaacgggaattctgcgaacgggaatgtgtagaagagaaaatgataaacgggaattctgttgcatcgatgatgacatcaaatgggaataatttctatttcttgccagcagggctcaagtccggatcgtttgtccggagagtgtccgcgtgcattattcagggacaattcacagcgaaccacacaaaccttcatgccgatttgtaaatgtctttcgatattgtacatagcaacttttgcaagtatacgttgacacgttgcgagcgccatcggaaagacagcccacttagtccagacaatgaaaccactatttatatcgcccacaaagccgaatcgacggtcttgttttccgcaggtatatc
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity/1
owned by: sophia
elements: cattctatgctatacggtacaagattgtggctactatgcaagctccatagcgaatgatcttacactgaccttgtcgaccgttctgaatgattaacaatggtcgcaagtgtggttgacccacctgcgggcgcttcttaactatctatcatcggtatgtcaagtatcactccctttaaacaaaagaataagagcataatacagcagtctcatcatggcaacccttcgcgcaatgtacaaaaagcagccagaagcccccacgcacaagcaataatgtcgtgttctaccctgccatgaacaagcgtcatggccattagcgaacgccccaatattcagccccattcgccaagcaagttgcctcgcccccttcccagaagatcatgggcatctcgtcttcttctaatttcgcattttgtgaagctccattcatgcacgtcggcttctggacgactcttatcatatgcattggcctgctcggtgtgaggatgagccactcccacatgtttagacggcaggggcctcttgccacccgttgaatttttggtgcgaagacgtgcctgggaacgacctccactttaaatttctccgttgttgcggtcgctccatcctcctcacatttcttgcctcctaagtcccatgtgccacactcggtgaaaaacggagtttgatacggatgaaatttccagtgagcttttcacattctggaggtttcgaaaaaaattgtgggattttctgaattaatccgagaatccgccctgcccatcaaccggaaagaattaaatccctcgcacattcctttctcccaccaccttctttatctctacattctttaaccaaaacacctaggtgagtacccaaagtcttttgacacctttgaaaatccgcgccagtcgtagggcacgtaaaacaattctcgctggtggcttctgcaggtgaagtcggggcagagtggagctgagcatggtcgaccacccacatcattctgtcttgtttttggcgatgtcttccatgcttttgccctcatctgttgcgtcgactgcagagtactgttttgccactactgagctcaacatgggcttgtgtgtggtgaagaaaaaaatttggtggctcttgaaaattttcaacccatcggatacatgcccttccaactcacaacgccctaacactttattgtcctatcattgcctcttcgcacatgctaacgatctccacagaaaacttcaaa
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_AE017346_Region_940695_942557Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_AE017346_Region_940695_942557_SBOLDesignerActivity/1
owned by: sophia
elements: tctagaaaccagtcattgaaacggtaacaagcataacttccgatgtagcttaccgcctgccactcgtcccactgttcatcacccgcttcgatcttatgctcaacctcgttccaattcatacccgacttttccaagacgtcactgattggattgatgtaattcttcatgagatagtttatgtggtgatacagataatcaccagtcaaacgtttctggatgagtttctgcatatccggatcatcgttgatttgttcttgcagatcagaacaagcggctttgtcggtaattttgaacttctcattggtacagctagaaatggggcacagttaattttcagtgaaaggtgatcgatagatactgaggaggacatacgattgataatcaatgatattatctaccacgaattcatcaagagcatggatgtgggcaaaagtcatagaattaccttggctgatagtgtgttgggtctgtgagccgccggttgttcccctttgcttccttttgcctcggtatcctttccccttgcccttccccttggttgctcggcctgtatttttgggtccatttgggccaagaggcggttgccaatcatatcttgatgcgactgtctcaagttgttggcttggttgtgcttccggcgttgtactggtaggggtggatacaatggagtattcaaaacgttcatccatggggacattacgttgctgctggcgagctggtagattgggatctagttggaatagtggattatggtgggcaacttcgtatgcgctcatttgagagagagggcaagattcattaagtgaggaaatggatgtcggaggcgagcgcacattatcatccgtgcaatgataagaagtgccaccaaaatggagaggacgagctgcagcatctggctgcgttggagaacgataggaggagacccacaagtgatttctcgagggtcgatgacttgtcgaagcaatagtaggagattgggaaggctggctggagccatggttggtttggtcggccaagggtataaagtgatgagaggatgcctgcattatcgcttcaagctggatttgagataataaatatgggtcataaatgtatggaaagttaatgcaaggttgccgagggaacttgttgaagagatacctcctcagtcctgtaactattataacgaaagatgagcgcgagtggagacgaaggattgatggaagatgcatgacggtatgacggcatctgctgatcttcgctgattacttctacctcagaggcaagaggtaggataatcattattactggaaggggagatttttggactctgattccgataaatgaacgattcaaacgctgatctgccgagcaccccctttatgtgcctgttgaaggtttatccgattcaactgagaaaaagcggtaaccaccgattcaacggctactacggtgttgtgccattttattccaacattgccgagcgacgcgaacacgccggaaaaatcacgtaggtagtgtggagaccccggtgcaacaagcgatgacttaaaggcaaatgccgcggggattttcgccatttgccgctgcattaccaaatgtggcagaggagtttatagaaagaacgagatggagggatttggaaacgaggcggaaacgaacgcatggggagtgaatcagcgaatagaaattcgcgcgagtcgactcgtgccctgtcgcttgcgtcatcgtagtagcccttcagcctttcaactttcccacattctcttgaattattttggctgatttgctttctcttttctctttctcttttcttccaacaatttaacaaactcttttgttctatccattgaatccttctaggagtatacttgcataaataca
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/CnU6Sequence/1
type: Sequence
persistent identity: CnU6Sequence
display id: CnU6Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/CnU6_terminator_SBOLDesignerActivity/1
owned by: sophia
elements: tttttt
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/amdSSequence/1
type: Sequence
persistent identity: amdSSequence
display id: amdSSequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/amdS_SBOLDesignerActivity/1
owned by: sophia
elements: atgcctcaatcctgggaagaactggccgctgataagcgcgcccgcctcgcaaaaaccatccctgatgaatggaaagtccagacgctgcctgcggaagacagcgttattgatttcccaaagaaatcggggatcctttcagaggccgaactgaagatcacagaggcctccgctgcagatcttgtgtccaagctggcggccggagagttgacctcggtggaagttacgctagcattctgtaaacgggcagcaatcgcccagcagttaacaaactgcgcccacgagttcttccctgacgccgctctcgcgcaggcaagggaactcgatgaatactacgcaaagcacaagagacccgttggtccactccatggcctccccatctctctcaaagaccagcttcgagtcaagggctacgaaacatcaatgggctacatctcatggctaaacaagtacgacgaaggggactcggttctgacaaccatgctccgcaaagccggtgccgtcttctacgtcaagacctctgtcccgcagaccctgatggtctgcgagacagtcaacaacatcatcgggcgcaccgtcaacccacgcaacaagaactggtcgtgcggcggcagttctggtggtgagggtgcgatcgttgggattcgtggtggcgtcatcggtgtaggaacggatatcggtggctcgattcgagtgccggccgcgttcaacttcctgtacggtctaaggccgagtcatgggcggctgccgtatgcaaagatggcgaacagcatggagggtcaggagacggtgcacagcgttgtcgggccgattacgcactctgttgaggacctccgcctcttcaccaaatccgtcctcggtcaggagccatggaaatacgactccaaggtcatccccatgccctggcgccagtccgagtcggacattattgcctccaagatcaagaacggcgggctcaatatcggctactacaacttcgacggcaatgtccttccacaccctcctatcctgcgcggcgtggaaaccaccgtcgccgcactcgccaaagccggtcacaccgtgaccccgtggacgccatacaagcacgatttcggccacgatctcatctcccatatctacgcggctgacggcagcgccgacgtaatgcgcgatatcagtgcatccggcgagccggcgattccaaatatcaaagacctactgaacccgaacatcaaagctgttaacatgaacgagctctgggacacgcatctccagaagtggaattaccagatggagtaccttgagaaatggcgggaggctgaagaaaaggccgggaaggaactggacgccatcatcgcgccgattacgcctaccgctgcggtacggcatgaccagttccggtactatgggtatgcctctgtgatcaacctgctggatttcacgagcgtggttgttccggttacctttgcggataagaacatcgataagaagaatgagagtttcaaggcggttagtgagcttgatgccctcgtgcaggaagagtatgatccggaggcgtaccatggggcaccggttgcagtgcaggttatcggacggagactcagtgaagagaggacgttggcgattgcagaggaagtggggaagttgctgggaaatgtggtgactccatag
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/1
owned by: sophia
elements: ttgcattagaactaaaaacaaagcatgattattacagttcatttattttttaaattgatcggcatgcatgcaaagtatacgtgcaaggacaatggtaacctgcaggtgtgaccgataattataaccatttgttgagaatgaagaggtgaggagaaaaacaatggatgacgggaaaaaaataaaaaaacactgagacggcgtggaccgccgtcttatttgcttccgttatccgccaaagtggaaattgcacatacaccggcagggtatactgtt
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_301512_302226Sequence/1
type: Sequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_301512_302226_SBOLDesignerActivity/1
owned by: sophia
elements: gatcttggggatggtattgaagacgatccagaaaaagtctctccatccctcccacgttcgctgttcggtttctttgcaaagagcgaagttgctcgacctttgacagaccctaaaatctggtcgtattttgcgttcaaagatcgcttactgcgaccgcgattttttagaggtggattttttgttgcgagagctagcgcatcggataggcatgggtgatatacgtttgcgttccttatgaggggtatcatcagtggcaaattaccgactagatgaagaagacgacctacaccccgctaaccggtgtccgatcgacatgatccacggtgtccaaagcgaagaaggacatgatgattggagttcgcttacctttggtataaaccagtgttgtttctgaagaggtgaggaagaaaatgatctagggcaataacagaaatcgtttgaagaagaagtaaaagaaaagaacacacgtcagacaagcatcatcatcattcattttgaatactcatcacggcaccttcttccttgcatgcgcattattcatgatgagatgctcgcagccgccggatttcggctgcaatcaagaacgatatcgcgtacggcgatgtccgcctccactccatcttccaacaaccaccacagtgcccgataacactacgacgaaaattgttcttttttggatcttgtcgacaacggcgaccaaaacaagaacttgatc
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GFPSequence/1
type: Sequence
persistent identity: GFPSequence
display id: GFPSequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/GFP_SBOLDesignerActivity/1
owned by: sophia
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactcacggcatggacgagctgtacaagtaa
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/mCherrySequence/1
type: Sequence
persistent identity: mCherrySequence
display id: mCherrySequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/mCherry_SBOLDesignerActivity/1
owned by: sophia
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaggctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgccgctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaa
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/Hygromicin_ResistanceSequence/1
type: Sequence
persistent identity: Hygromicin ResistanceSequence
display id: Hygromicin_ResistanceSequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Hygromicin_Resistance_SBOLDesignerActivity/1
owned by: sophia
elements: atgaaaaagcctgaactcaccgcgacgtctgtcgagaagtttctgatcgaaaagttcgacagcgtctccgacctgatgcagctctcggagggcgaagaatctcgtgctttcagcttcgatgtaggagggcgtggatatgtcctgcgggtaaatagctgcgccgatggtttctacaaagatcgttatgtttatcggcactttgcatcggccgcgctcccgattccggaagtgcttgacattggggaattcagcgagagcctgacctattgcatctcccgccgtgcacagggtgtcacgttgcaagacctgcctgaaaccgaactgcccgctgttctgcagccggtcgcggaggccatggatgcgatcgctgcggccgatcttagccagacgagcgggttcggcccattcggaccgcaaggaatcggtcaatacactacatggcgtgatttcatatgcgcgattgctgatccccatgtgtatcactggcaaactgtgatggacgacaccgtcagtgcgtccgtcgcgcaggctctcgatgagctgatgctttgggccgaggactgccccgaagtccggcacctcgtgcacgcggatttcggctccaacaatgtcctgacggacaatggccgcataacagcggtcattgactggagcgaggcgatgttcggggattcccaatacgaggtcgccaacatcttcttctggaggccgtggttggcttgtatggagcagcagacgcgctacttcgagcggaggcatccggagcttgcaggatcgccgcggctccgggcgtatatgctccgcattggtcttgaccaactctatcagagcttggttgacggcaatttcgatgatgcagcttgggcgcagggtcgatgcgacgcaatcgtccgatccggagccgggactgtcgggcgtacacaaatcgcccgcagaagcgcggccgtctggaccgatggctgtgtagaagtactcgccgatagtggaaaccgacgccccagcactcgtccgagggcaaaggaatag
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/Neomicin_ResistanceSequence/1
type: Sequence
persistent identity: Neomicin ResistanceSequence
display id: Neomicin_ResistanceSequence
version: 1
was generated by: https://synbiohub.org/public/CnDatabase/Neomicin_Resistance_SBOLDesignerActivity/1
owned by: sophia
elements: atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatcccaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctga
encoding: naseq.html

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/1
type: Activity
persistent identity: GenBank CP003831 Region 176453 177430 SBOLDesignerActivity
display id: GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-10T20:27:10.510Z
qualified association: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_176453_177430_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/CnU6_terminator_SBOLDesignerActivity/1
type: Activity
persistent identity: CnU6 terminator SBOLDesignerActivity
display id: CnU6_terminator_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2022-06-10T21:33:45.697Z
qualified association: https://synbiohub.org/public/CnDatabase/CnU6_terminator_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/CnU6_terminator_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/CnU6_terminator_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/amdS_SBOLDesignerActivity/1
type: Activity
persistent identity: amdS SBOLDesignerActivity
display id: amdS_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-12T15:44:48.638Z
qualified association: https://synbiohub.org/public/CnDatabase/amdS_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/amdS_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/amdS_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/mCherry_SBOLDesignerActivity/1
type: Activity
persistent identity: mCherry SBOLDesignerActivity
display id: mCherry_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2022-06-10T21:02:52.508Z
qualified association: https://synbiohub.org/public/CnDatabase/mCherry_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/mCherry_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/mCherry_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity/1
type: Activity
persistent identity: GenBank CM000046 Region 303006 303540 SBOLDesignerActivity
display id: GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-11T20:25:23.374Z
qualified association: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GenBank_CM000046_Region_303006_303540_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/1
type: Activity
persistent identity: Cas9 SBOLDesignerActivity
display id: Cas9_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-12T16:15:32.139Z
qualified association: https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/Cas9_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity/1
type: Activity
persistent identity: GenBank CP003820 Region 1242235 1243067 SBOLDesignerActivity
display id: GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-11-01T03:55:50.845Z
qualified association: https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GenBank_CP003820_Region_1242235_1243067_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity/1
type: Activity
persistent identity: GenBank CP003831 Region 377755 378976 SBOLDesignerActivity
display id: GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-11T21:41:41.306Z
qualified association: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GenBank_CP003831_Region_377755_378976_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/GFP_SBOLDesignerActivity/1
type: Activity
persistent identity: GFP SBOLDesignerActivity
display id: GFP_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2022-06-10T20:37:16.641Z
qualified association: https://synbiohub.org/public/CnDatabase/GFP_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GFP_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GFP_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity/1
type: Activity
persistent identity: GenBank CP047908 Region 1244167 1244926 SBOLDesignerActivity
display id: GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-11-01T18:51:50.155Z
qualified association: https://synbiohub.org/public/CnDatabase/GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GenBank_CP047908_Region_1244167_1244926_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/1
type: Activity
persistent identity: Nourseothricin resistance SBOLDesignerActivity
display id: Nourseothricin_resistance_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-13T21:16:02.486Z
qualified association: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/Nourseothricin_resistance_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/1
type: Activity
persistent identity: GenBank AE017348 Region 871991 872263 SBOLDesignerActivity
display id: GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity
version: 1
creator: Sophia Garcia de Resende
owned by: sophia
ended at time: 2021-12-10T15:39:07.013Z
qualified association: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/Association/1

https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/Association/1
type: Association
persistent identity: Association
display id: Association
version: 1
owned by: sophia
top level: https://synbiohub.org/public/CnDatabase/GenBank_AE017348_Region_871991_872263_SBOLDesignerActivity/1
had role: sequenceEditor
agent: SBOLDesigner CAD Tool

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SBOLDesigner CAD Tool
type: Agent
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description: SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
creator: Bryan Bartley
creator: Chris Myers
creator: Evren Sirin
creator: John Gennari
creator: Michael Zhang
creator: Michal Galdzicki
creator: Samuel Bridge
creator: Sean Sleight
owned by: myers