GenBank_CP003831_Region_176110_177085 1 GAL1 Organism: Cryptococcus neoformans var. grubii H99 2022-06-18T14:37:57 Genbank: <a href="https://www.ncbi.nlm.nih.gov/nucleotide/CP003831.1?report=genbank&log$=nuclalign&blast_rank=4&RID=TNYRTZMN01N&from=176110&to=177085">CP003831 Region: 176110..177085</a> This is the galactose kinase promoter. This promoter, GAL1 + 5'UTR, is repressible by glucose and inducible by galactose. The 3' primer used to amplify the GAL1 promoter region encompassed the first codon ATG. This ATG codon was, therefore, excluded from the promoter presented here, diminishing the size of this promoter from 978 to 976 bp. 18952189 GenBank_CP003831_Region_176110_177085Sequence 1 ctattaaagatcgaagatcagctatgccggtccatgccatgtatatggacttaccctacttcattttgggctatttgattgcagcgctcgagagcttgagggtatgcgttgtgcgcattgtcaatgatgatgggcaagtagcgaccagagaccagaagtgagaggacaacgtgagatcctatgtatccgagtccaccggtgacaaggacgttctgtaatgagaattagccttggttttgttgtaattgtaatagaatggtcccactttcaaaggctgtccatggacagacaagcttgatgtcgacgtttggttcatggtaactcgagtctgttcaaaaaccataatgcaggtgagcggttgagtgtgttattgcgattcaatgtttgctatcatagctcatggtcattgaggctgcattctacatcttttatactggatgctgacatcctcttcccaacacctattagggaaagtcgatgcaacagaatcatgaacgggaatactcagaattggacccgagcacgaacgggaatcttgcccaaataatgctttgttcgaacgggaatttcgaggatgggcaattctgcgaacgggaatgtgtagtagaggaagcgatgaacgggaattctgcgaacgggaatgtgtagaagagaaaatgataaacgggaattctgttgcatcgatgatgacatcaaatgggaataatttctatttcttgccagcagggctcaagtccggatcgtttgtccggagagtgtccgcgtgcattattcagggacaattcacagcgaaccacacaaaccttcatgccgatttgtaaatgtctttcgatattgtacatagcaacttttgcaagtatacgttgacacgttgcgagcgccatcggaaagacagcccacttagtccagacaatgaaaccactatttatatcgcccacaaagccgaatcgacggtcttgttttccgcaggtatatc GenBank_CP003831_Region_176110_177085_SBOLDesignerActivity 1 Sophia Garcia de Resende 2022-06-10T14:59:37.626Z Association 1 SBOLDesigner 3.1 SBOLDesigner CAD Tool SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences. Samuel Bridge Chris Myers Michal Galdzicki Michael Zhang John Gennari Sean Sleight Bryan Bartley Evren Sirin