Restriction_sites 1 Restriction_sites SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI MCS 1 MCS PBSComponent 1 Restriction_sitesComponent 1 MCSSequenceAnnotation1 1 MCSSequenceAnnotation1Range 1 60 83 MCSSequenceAnnotation 1 MCSSequenceAnnotationRange 1 1 59 SequenceConstraint 1 PBS_F24 1 PBS_F24 PBS_F24Sequence 1 gtcgtgactgggaaaaccctggcg Restriction_sitesSequence 1 gagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgc MCSSequence 1 gagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgcgtcgtgactgggaaaaccctggcg