LOCUS all_or_nothing_ 3967 bp DNA linear 05-May-2024 ACCESSION all_or_nothing_Pm VERSION all_or_nothing_Pm.1 FEATURES Location/Qualifiers promoter 1..1987 /label=XylSPm misc_feature 1988..1997 /label=RBS_cassette misc_feature 1998..2008 /label=Spacer CDS 2009..3088 /label=LacI misc_feature 3089..3125 /label=Coupling CDS 1998..3125 /label=LacI_cassette misc_feature 3126..3128 /label=ATG misc_feature 3129..3134 /label=NheI CDS 3135..3848 /label=msfGFP misc_feature 3849..3854 /label=EcoRI CDS 3126..3854 /label=gfp_cassette misc_feature 3855..3913 /label=Restriction_sites primer_bind 3914..3937 /label=PBS_F24 misc_feature 3855..3937 /label=MCS terminator 3938..3967 /label=T500 ORIGIN 1 acgttcgtaa tcaagccact tcctttttgc attgacgcag ggtgtcggaa ggcaactcgc 61 cgaacgcgct cctatagttt tcagcgaagc gtcccaaatg taagaagccg tagtctaggg 121 ctatctcagt tatactacgc acattggcac tgggatcgtt caagcaggcg cggatgcttt 181 cgagcttgcg gttgcggatg tagttcttcg gcgtggtgcc ggcgtgcttc tcgaacaaat 241 tgtagagcga gcgtggactc atcatcgcca gctccgctaa ccgctcaagg ctgatattcc 301 gtttgagatt ctcctcaatg aattgaacga ctcgctcgaa agacgggtta cctttgctga 361 aaatttcacg gctgacattg ctgcccagca tttcgagcag cttggaagcg atgatccccg 421 catagtgctc ttggacccga ggcatcgact ttgtatgttc cgcttcgtca caaactaacc 481 cgagtagatt gataaagcca tcgagttgct ggagattgtg tcgcgcggcg aaacggatac 541 cctccctcgg cttgtgccaa ttgttgtcac tgcacgcccg atcaaggacc actgagggca 601 atttaacgat aaatttctcg caatcttctg aataggtcag gtcggcttgg tcatccggat 661 tgagcagcaa tagttcgccc ggcgcaaaat agtgctcctg gccatggcca cgccacaggc 721 aatggccttt gagtattatt tgcagatgat aacaggtctc taatccaggc gagattaccc 781 tcacgctacc gccgtagctg attcgacaca gatcgaggca tccgaagatt ctgtggtgca 841 gcctgcctgc cgggcgcccg cccttgggca ggcgaataga gtgcgtaccg acatactggt 901 taacataatc ggagactgca tagggctcgg cgtggacgaa gatctgactt ttctcgttca 961 ataagcaaaa atccatagtt cacggttctc ttattttaat gtgggctgct tggtgtgatg 1021 tagaaaggcg ccaagtcgat gaaaatgcat ctcgacgtga tgcgtatacg ggttaccccc 1081 attgccacgt tgcgccatcc tttttgcaat cagtgaccac ttttccaagc aaaaataacg 1141 ccaagcagaa cgaagacgtt ctttttaaga agcgagaaca ccagaagttc gtgctgtcgg 1201 ggcatggggc gacgaattgg cggataaagg ggatctgctg gatattacgg cctttttaaa 1261 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct 1321 gatgaatgct catccgtaat tacgtatggc aatgaaagac ggtgagctgg tgatatggga 1381 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg 1441 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg 1501 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc 1561 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt 1621 cttcgccccc gttttcacca tgcatgggaa ttagcttgat ctgaccaacg accggtagcg 1681 gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga acagtaccat 1741 gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc tccatgacaa 1801 atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaag tgcagtgtcc 1861 ggtttgatag ggataagtcc agccttgcaa gaagcggata caggagtgca aaaaatggct 1921 atctctagta aggcctaccc cttaggcttt atgcaacaga aacaataata atggagtcat 1981 gaccatgcct aggaggaaaa aacatatggt gaaaccagta acgttatacg atgtcgcaga 2041 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc 2101 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg 2161 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct 2221 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg 2281 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt 2341 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca 2401 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc 2461 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt 2521 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc 2581 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca 2641 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca 2701 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct 2761 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt 2821 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca 2881 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca 2941 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc 3001 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 3061 acaggtttcc cgactggaaa gcgggcagca tcatcaccac catcattagg atggtggtga 3121 tgataatggc tagccgtaaa ggtgaagaac tgttcaccgg tgttgttccg atcctggttg 3181 aactggatgg tgatgttaac ggccacaaat tctctgttcg tggtgaaggt gaaggtgatg 3241 caaccaacgg taaactgacc ctgaaattca tctgcactac cggtaaactg ccggttccat 3301 ggccgactct ggtgactacc ctgacctatg gtgttcagtg tttttctcgt tacccggatc 3361 acatgaagca gcatgatttc ttcaaatctg caatgccgga aggttatgta caggagcgca 3421 ccatttcttt caaagacgat ggcacctaca aaacccgtgc agaggttaaa tttgaaggtg 3481 atactctggt gaaccgtatt gaactgaaag gcattgattt caaagaggac ggcaacatcc 3541 tgggccacaa actggaatat aacttcaact cccataacgt ttacatcacc gcagacaaac 3601 agaagaacgg tatcaaagct aacttcaaaa ttcgccataa cgttgaagac ggtagcgtac 3661 agctggcgga ccactaccag cagaacactc cgatcggtga tggtccggtt ctgctgccgg 3721 ataaccacta cctgtccacc cagtctaaac tgtccaaaga cccgaacgaa aagcgcgacc 3781 acatggtgct gctggagttc gttactgccg caggtatcac gcacggcatg gatgaactct 3841 acaaataaga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca 3901 agcttgcggc cgcgtcgtga ctgggaaaac cctggcgcaa agcccgccga aaggcgggct 3961 tttctgt //