all_or_nothing_Pm
type: ComponentDefinition
persistent identity: all or nothing Pm
display id: all_or_nothing_Pm
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/1
sequence annotation: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint2/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint3/1
sequence constraint: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint4/1
sequence: https://synbiohub.org/public/Digitalizer/all_or_nothing_PmSequence/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1
type: Component
persistent identity: TerComponent
display id: TerComponent
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
definition: T500
access: public

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1
type: Component
persistent identity: CDS2Component
display id: CDS2Component
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
definition: gfp_cassette
access: public

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1
type: Component
persistent identity: MCSComponent
display id: MCSComponent
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
definition: MCS
access: public

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1
type: Component
persistent identity: CDSComponent
display id: CDSComponent
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
definition: LacI_cassette
access: public

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1
type: Component
persistent identity: RBSComponent
display id: RBSComponent
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
definition: RBS_cassette
access: public

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1
type: Component
persistent identity: Pro2Component
display id: Pro2Component
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
definition: XylSPm
access: public

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation
display id: all_or_nothing_PmSequenceAnnotation
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/all_or_nothing_PmSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation/all_or_nothing_PmSequenceAnnotationRange/1
type: Range
persistent identity: all or nothing PmSequenceAnnotationRange
display id: all_or_nothing_PmSequenceAnnotationRange
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
start: 1
end: 1987
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation1
display id: all_or_nothing_PmSequenceAnnotation1
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/all_or_nothing_PmSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation1/all_or_nothing_PmSequenceAnnotation1Range/1
type: Range
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
start: 1988
end: 1997
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation2
display id: all_or_nothing_PmSequenceAnnotation2
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/all_or_nothing_PmSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation2/all_or_nothing_PmSequenceAnnotation2Range/1
type: Range
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
start: 1998
end: 3125
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation3
display id: all_or_nothing_PmSequenceAnnotation3
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/all_or_nothing_PmSequenceAnnotation3Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation3/all_or_nothing_PmSequenceAnnotation3Range/1
type: Range
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
start: 3126
end: 3854
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation4
display id: all_or_nothing_PmSequenceAnnotation4
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/all_or_nothing_PmSequenceAnnotation4Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation4/all_or_nothing_PmSequenceAnnotation4Range/1
type: Range
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
start: 3855
end: 3937
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/1
type: SequenceAnnotation
persistent identity: all or nothing PmSequenceAnnotation5
display id: all_or_nothing_PmSequenceAnnotation5
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/all_or_nothing_PmSequenceAnnotation5Range/1
component: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/all_or_nothing_PmSequenceAnnotation5/all_or_nothing_PmSequenceAnnotation5Range/1
type: Range
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
start: 3938
end: 3967
orientation: inline

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/Pro2Component/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
top level: all_or_nothing_Pm
owned by: Angel
owned by: myers
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/RBSComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint2/1
type: SequenceConstraint
persistent identity: SequenceConstraint2
display id: SequenceConstraint2
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDSComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint3/1
type: SequenceConstraint
persistent identity: SequenceConstraint3
display id: SequenceConstraint3
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/CDS2Component/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/SequenceConstraint4/1
type: SequenceConstraint
persistent identity: SequenceConstraint4
display id: SequenceConstraint4
version: 1
top level: all_or_nothing_Pm
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/MCSComponent/1
object: https://synbiohub.org/public/Digitalizer/all_or_nothing_Pm/TerComponent/1

LacI_cassette
type: ComponentDefinition
persistent identity: LacI cassette
display id: LacI_cassette
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/1
sequence constraint: https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint1/1
sequence: https://synbiohub.org/public/Digitalizer/LacI_cassetteSequence/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1
type: Component
persistent identity: CouplingComponent
display id: CouplingComponent
version: 1
top level: LacI_cassette
owned by: Angel
owned by: myers
definition: Coupling
access: public

https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1
type: Component
persistent identity: CDS1Component
display id: CDS1Component
version: 1
top level: LacI_cassette
owned by: Angel
owned by: myers
definition: LacI
access: public

https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1
type: Component
persistent identity: SpacerComponent
display id: SpacerComponent
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
definition: Spacer
access: public

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: LacI cassetteSequenceAnnotation
display id: LacI_cassetteSequenceAnnotation
version: 1
top level: LacI_cassette
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/LacI_cassetteSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation/LacI_cassetteSequenceAnnotationRange/1
type: Range
persistent identity: LacI cassetteSequenceAnnotationRange
display id: LacI_cassetteSequenceAnnotationRange
version: 1
top level: LacI_cassette
owned by: Angel
owned by: myers
start: 1
end: 11
orientation: inline

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: LacI cassetteSequenceAnnotation1
display id: LacI_cassetteSequenceAnnotation1
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
location: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/LacI_cassetteSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation1/LacI_cassetteSequenceAnnotation1Range/1
type: Range
persistent identity: LacI cassetteSequenceAnnotation1Range
display id: LacI_cassetteSequenceAnnotation1Range
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
start: 12
end: 1091
orientation: inline

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: LacI cassetteSequenceAnnotation2
display id: LacI_cassetteSequenceAnnotation2
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
location: https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/LacI_cassetteSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/LacI_cassetteSequenceAnnotation2/LacI_cassetteSequenceAnnotation2Range/1
type: Range
persistent identity: LacI cassetteSequenceAnnotation2Range
display id: LacI_cassetteSequenceAnnotation2Range
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
start: 1092
end: 1128
orientation: inline

https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/LacI_cassette/SpacerComponent/1
object: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1

https://synbiohub.org/public/Digitalizer/LacI_cassette/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
top level: LacI_cassette
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/LacI_cassette/CDS1Component/1
object: https://synbiohub.org/public/Digitalizer/LacI_cassette/CouplingComponent/1

MCS
type: ComponentDefinition
persistent identity: MCS
display id: MCS
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000804
component: https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1
component: https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/1
sequence constraint: https://synbiohub.org/public/Digitalizer/MCS/SequenceConstraint/1
sequence: https://synbiohub.org/public/Digitalizer/MCSSequence/1

https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1
type: Component
persistent identity: PBSComponent
display id: PBSComponent
version: 1
top level: MCS
owned by: myers
owned by: Angel
definition: PBS_F24
access: public

https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1
type: Component
persistent identity: Restriction sitesComponent
display id: Restriction_sitesComponent
version: 1
top level: MCS
owned by: Angel
owned by: myers
definition: Restriction_sites
access: public

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: MCSSequenceAnnotation
display id: MCSSequenceAnnotation
version: 1
top level: MCS
owned by: myers
owned by: Angel
location: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/MCSSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation/MCSSequenceAnnotationRange/1
type: Range
persistent identity: MCSSequenceAnnotationRange
display id: MCSSequenceAnnotationRange
version: 1
top level: MCS
owned by: myers
owned by: Angel
start: 1
end: 59
orientation: inline

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: MCSSequenceAnnotation1
display id: MCSSequenceAnnotation1
version: 1
top level: MCS
owned by: myers
owned by: Angel
location: https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/MCSSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1

https://synbiohub.org/public/Digitalizer/MCS/MCSSequenceAnnotation1/MCSSequenceAnnotation1Range/1
type: Range
persistent identity: MCSSequenceAnnotation1Range
display id: MCSSequenceAnnotation1Range
version: 1
top level: MCS
owned by: myers
owned by: Angel
start: 60
end: 83
orientation: inline

https://synbiohub.org/public/Digitalizer/MCS/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
top level: MCS
owned by: Angel
owned by: myers
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/MCS/Restriction_sitesComponent/1
object: https://synbiohub.org/public/Digitalizer/MCS/PBSComponent/1

RBS_cassette
type: ComponentDefinition
persistent identity: RBS cassette
display id: RBS_cassette
version: 1
description: AvrII restriction site (cctagg), overlapped with RBS (aggagga)
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000110
sequence: https://synbiohub.org/public/Digitalizer/RBS_cassetteSequence/1

T500
type: ComponentDefinition
persistent identity: T500
display id: T500
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000141
sequence: https://synbiohub.org/public/Digitalizer/T500Sequence/1

XylSPm
type: ComponentDefinition
persistent identity: XylSPm
display id: XylSPm
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000167
sequence: https://synbiohub.org/public/Digitalizer/XylSPmSequence/1

gfp_cassette
type: ComponentDefinition
persistent identity: gfp cassette
display id: gfp_cassette
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000316
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/1
sequence annotation: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/1
sequence constraint: https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint2/1
sequence constraint: https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint/1
sequence constraint: https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint1/1
sequence: https://synbiohub.org/public/Digitalizer/gfp_cassetteSequence/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1
type: Component
persistent identity: CUT3Component
display id: CUT3Component
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
definition: EcoRI
access: public

https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1
type: Component
persistent identity: CUT2Component
display id: CUT2Component
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
definition: NheI
access: public

https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1
type: Component
persistent identity: CDS3Component
display id: CDS3Component
version: 1
top level: gfp_cassette
owned by: Angel
owned by: myers
definition: msfGFP
access: public

https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1
type: Component
persistent identity: ATGComponent
display id: ATGComponent
version: 1
top level: gfp_cassette
owned by: Angel
owned by: myers
definition: ATG
access: public

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation
display id: gfp_cassetteSequenceAnnotation
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/gfp_cassetteSequenceAnnotationRange/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation/gfp_cassetteSequenceAnnotationRange/1
type: Range
persistent identity: gfp cassetteSequenceAnnotationRange
display id: gfp_cassetteSequenceAnnotationRange
version: 1
top level: gfp_cassette
owned by: Angel
owned by: myers
start: 1
end: 3
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation1
display id: gfp_cassetteSequenceAnnotation1
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/gfp_cassetteSequenceAnnotation1Range/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation1/gfp_cassetteSequenceAnnotation1Range/1
type: Range
persistent identity: gfp cassetteSequenceAnnotation1Range
display id: gfp_cassetteSequenceAnnotation1Range
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
start: 4
end: 9
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation2
display id: gfp_cassetteSequenceAnnotation2
version: 1
top level: gfp_cassette
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/gfp_cassetteSequenceAnnotation2Range/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation2/gfp_cassetteSequenceAnnotation2Range/1
type: Range
persistent identity: gfp cassetteSequenceAnnotation2Range
display id: gfp_cassetteSequenceAnnotation2Range
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
start: 10
end: 723
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/1
type: SequenceAnnotation
persistent identity: gfp cassetteSequenceAnnotation3
display id: gfp_cassetteSequenceAnnotation3
version: 1
top level: gfp_cassette
owned by: Angel
owned by: myers
location: https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/gfp_cassetteSequenceAnnotation3Range/1
component: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/gfp_cassetteSequenceAnnotation3/gfp_cassetteSequenceAnnotation3Range/1
type: Range
persistent identity: gfp cassetteSequenceAnnotation3Range
display id: gfp_cassetteSequenceAnnotation3Range
version: 1
top level: gfp_cassette
owned by: Angel
owned by: myers
start: 724
end: 729
orientation: inline

https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint2/1
type: SequenceConstraint
persistent identity: SequenceConstraint2
display id: SequenceConstraint2
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1
object: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT3Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint/1
type: SequenceConstraint
persistent identity: SequenceConstraint
display id: SequenceConstraint
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/gfp_cassette/ATGComponent/1
object: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1

https://synbiohub.org/public/Digitalizer/gfp_cassette/SequenceConstraint1/1
type: SequenceConstraint
persistent identity: SequenceConstraint1
display id: SequenceConstraint1
version: 1
top level: gfp_cassette
owned by: myers
owned by: Angel
restriction: precedes
subject: https://synbiohub.org/public/Digitalizer/gfp_cassette/CUT2Component/1
object: https://synbiohub.org/public/Digitalizer/gfp_cassette/CDS3Component/1

Coupling
type: ComponentDefinition
persistent identity: Coupling
display id: Coupling
version: 1
description: Translational coupler
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/CouplingSequence/1

LacI
type: ComponentDefinition
persistent identity: LacI
display id: LacI
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/Digitalizer/LacISequence/1

PBS_F24
type: ComponentDefinition
persistent identity: PBS F24
display id: PBS_F24
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0005850
sequence: https://synbiohub.org/public/Digitalizer/PBS_F24Sequence/1

Restriction_sites
type: ComponentDefinition
persistent identity: Restriction sites
display id: Restriction_sites
version: 1
description: SacI - KpnI - SmaI - BamHI - XbaI - SalI - PstI - SphI - HindIII - NotI
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/Restriction_sitesSequence/1

Spacer
type: ComponentDefinition
persistent identity: Spacer
display id: Spacer
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/SpacerSequence/1

ATG
type: ComponentDefinition
persistent identity: ATG
display id: ATG
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0000804
sequence: https://synbiohub.org/public/Digitalizer/ATGSequence/1

EcoRI
type: ComponentDefinition
persistent identity: EcoRI
display id: EcoRI
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0001692
sequence: https://synbiohub.org/public/Digitalizer/EcoRISequence/1

NheI
type: ComponentDefinition
persistent identity: NheI
display id: NheI
version: 1
owned by: myers
owned by: Angel
type: DnaRegion
role: SO:0001692
sequence: https://synbiohub.org/public/Digitalizer/NheISequence/1

msfGFP
type: ComponentDefinition
persistent identity: msfGFP
display id: msfGFP
version: 1
owned by: Angel
owned by: myers
type: DnaRegion
role: SO:0000316
sequence: https://synbiohub.org/public/Digitalizer/msfGFPSequence/1

https://synbiohub.org/public/Digitalizer/all_or_nothing_PmSequence/1
type: Sequence
persistent identity: all or nothing PmSequence
display id: all_or_nothing_PmSequence
version: 1
owned by: Angel
owned by: myers
elements: acgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctcctatagttttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcactgggatcgttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgcttctcgaacaaattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgagattctcctcaatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgcccagcatttcgagcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttcgtcacaaactaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctccctcggcttgtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaatcttctgaataggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggccatggccacgccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctcacgctaccgccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgcccttgggcaggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaagatctgacttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgtaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaattagcttgatctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcagaacagtaccatgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctccccaactaatgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatgcctaggaggaaaa…
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/LacI_cassetteSequence/1
type: Sequence
persistent identity: LacI cassetteSequence
display id: LacI_cassetteSequence
version: 1
owned by: myers
owned by: Angel
elements: aaaaacatatggtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagcatcatcaccaccatcattaggatggtggtgatgata
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/MCSSequence/1
type: Sequence
persistent identity: MCSSequence
display id: MCSSequence
version: 1
owned by: myers
owned by: Angel
elements: gagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgcgtcgtgactgggaaaaccctggcg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/RBS_cassetteSequence/1
type: Sequence
persistent identity: RBS cassetteSequence
display id: RBS_cassetteSequence
version: 1
owned by: myers
owned by: Angel
elements: cctaggagga
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/T500Sequence/1
type: Sequence
persistent identity: T500Sequence
display id: T500Sequence
version: 1
owned by: myers
owned by: Angel
elements: caaagcccgccgaaaggcgggcttttctgt
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/XylSPmSequence/1
type: Sequence
persistent identity: XylSPmSequence
display id: XylSPmSequence
version: 1
owned by: myers
owned by: Angel
elements: acgttcgtaatcaagccacttcctttttgcattgacgcagggtgtcggaaggcaactcgccgaacgcgctcctatagttttcagcgaagcgtcccaaatgtaagaagccgtagtctagggctatctcagttatactacgcacattggcactgggatcgttcaagcaggcgcggatgctttcgagcttgcggttgcggatgtagttcttcggcgtggtgccggcgtgcttctcgaacaaattgtagagcgagcgtggactcatcatcgccagctccgctaaccgctcaaggctgatattccgtttgagattctcctcaatgaattgaacgactcgctcgaaagacgggttacctttgctgaaaatttcacggctgacattgctgcccagcatttcgagcagcttggaagcgatgatccccgcatagtgctcttggacccgaggcatcgactttgtatgttccgcttcgtcacaaactaacccgagtagattgataaagccatcgagttgctggagattgtgtcgcgcggcgaaacggataccctccctcggcttgtgccaattgttgtcactgcacgcccgatcaaggaccactgagggcaatttaacgataaatttctcgcaatcttctgaataggtcaggtcggcttggtcatccggattgagcagcaatagttcgcccggcgcaaaatagtgctcctggccatggccacgccacaggcaatggcctttgagtattatttgcagatgataacaggtctctaatccaggcgagattaccctcacgctaccgccgtagctgattcgacacagatcgaggcatccgaagattctgtggtgcagcctgcctgccgggcgcccgcccttgggcaggcgaatagagtgcgtaccgacatactggttaacataatcggagactgcatagggctcggcgtggacgaagatctgacttttctcgttcaataagcaaaaatccatagttcacggttctcttattttaatgtgggctgcttggtgtgatgtagaaaggcgccaagtcgatgaaaatgcatctcgacgtgatgcgtatacgggttacccccattgccacgttgcgccatcctttttgcaatcagtgaccacttttccaagcaaaaataacgccaagcagaacgaagacgttctttttaagaagcgagaacaccagaagttcgtgctgtcggggcatggggcgacgaattggcggataaaggggatctgctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgtaattacgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgcatgggaattagcttgatctgaccaacgaccggtagcggagctatccaacggcggtataccaggaaaacacacagcaggtacatcagaacagtaccatgactgaagaacaaatagttttttcctgatccataaagcagaacggcctgctccatgacaaatctggctccccaactaatgccccatgcagccagcataaccagcataaagtgcagtgtccggtttgatagggataagtccagccttgcaagaagcggatacaggagtgcaaaaaatggctatctctagtaaggcctaccccttaggctttatgcaacagaaacaataataatggagtcatgaccatg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/gfp_cassetteSequence/1
type: Sequence
persistent identity: gfp cassetteSequence
display id: gfp_cassetteSequence
version: 1
owned by: Angel
owned by: myers
elements: atggctagccgtaaaggtgaagaactgttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataagaattc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/CouplingSequence/1
type: Sequence
persistent identity: CouplingSequence
display id: CouplingSequence
version: 1
owned by: Angel
owned by: myers
elements: catcatcaccaccatcattaggatggtggtgatgata
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/LacISequence/1
type: Sequence
persistent identity: LacISequence
display id: LacISequence
version: 1
owned by: Angel
owned by: myers
elements: gtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/PBS_F24Sequence/1
type: Sequence
persistent identity: PBS F24Sequence
display id: PBS_F24Sequence
version: 1
owned by: myers
owned by: Angel
elements: gtcgtgactgggaaaaccctggcg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/Restriction_sitesSequence/1
type: Sequence
persistent identity: Restriction sitesSequence
display id: Restriction_sitesSequence
version: 1
owned by: myers
owned by: Angel
elements: gagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttgcggccgc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/SpacerSequence/1
type: Sequence
persistent identity: SpacerSequence
display id: SpacerSequence
version: 1
owned by: Angel
owned by: myers
elements: aaaaacatatg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/ATGSequence/1
type: Sequence
persistent identity: ATGSequence
display id: ATGSequence
version: 1
owned by: Angel
owned by: myers
elements: atg
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/EcoRISequence/1
type: Sequence
persistent identity: EcoRISequence
display id: EcoRISequence
version: 1
owned by: Angel
owned by: myers
elements: gaattc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/NheISequence/1
type: Sequence
persistent identity: NheISequence
display id: NheISequence
version: 1
owned by: myers
owned by: Angel
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/Digitalizer/msfGFPSequence/1
type: Sequence
persistent identity: msfGFPSequence
display id: msfGFPSequence
version: 1
owned by: Angel
owned by: myers
elements: cgtaaaggtgaagaactgttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataa
encoding: naseq.html