EcoRI
1
EcoRI
NheI
1
NheI
gfp_cassette
1
gfp_cassette
ATGComponent
1
CUT2Component
1
CUT3Component
1
CDS3Component
1
gfp_cassetteSequenceAnnotation3
1
gfp_cassetteSequenceAnnotation3Range
1
724
729
gfp_cassetteSequenceAnnotation
1
gfp_cassetteSequenceAnnotationRange
1
1
3
gfp_cassetteSequenceAnnotation2
1
gfp_cassetteSequenceAnnotation2Range
1
10
723
gfp_cassetteSequenceAnnotation1
1
gfp_cassetteSequenceAnnotation1Range
1
4
9
SequenceConstraint
1
SequenceConstraint1
1
SequenceConstraint2
1
ATG
1
ATG
msfGFP
1
msfGFP
ATGSequence
1
atg
EcoRISequence
1
gaattc
msfGFPSequence
1
cgtaaaggtgaagaactgttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataa
gfp_cassetteSequence
1
atggctagccgtaaaggtgaagaactgttcaccggtgttgttccgatcctggttgaactggatggtgatgttaacggccacaaattctctgttcgtggtgaaggtgaaggtgatgcaaccaacggtaaactgaccctgaaattcatctgcactaccggtaaactgccggttccatggccgactctggtgactaccctgacctatggtgttcagtgtttttctcgttacccggatcacatgaagcagcatgatttcttcaaatctgcaatgccggaaggttatgtacaggagcgcaccatttctttcaaagacgatggcacctacaaaacccgtgcagaggttaaatttgaaggtgatactctggtgaaccgtattgaactgaaaggcattgatttcaaagaggacggcaacatcctgggccacaaactggaatataacttcaactcccataacgtttacatcaccgcagacaaacagaagaacggtatcaaagctaacttcaaaattcgccataacgttgaagacggtagcgtacagctggcggaccactaccagcagaacactccgatcggtgatggtccggttctgctgccggataaccactacctgtccacccagtctaaactgtccaaagacccgaacgaaaagcgcgaccacatggtgctgctggagttcgttactgccgcaggtatcacgcacggcatggatgaactctacaaataagaattc
NheISequence
1
gctagc