LOCUS IBMc050 4567 bp ds-DNA circular 09-OCT-2020 DEFINITION pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI ACCESSION IBMc050 VERSION IBMc050.1 FEATURES Location/Qualifiers misc_feature 1100..1121 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" misc_feature 3610..3630 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 3437..3598 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" RBS 3415..3427 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" promoter 3353..3406 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" terminator complement(1543..1599) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" terminator complement(2394..2450) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" promoter complement(2288..2385) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" RBS complement(3246..3260) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 3304..3344 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" CDS complement(1628..2287) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 1134..1433 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (rev)" CDS complement(2484..3239) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" promoter complement(3269..3303) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 1445..1534 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" primer_bind complement(3688..3707) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" rep_origin complement(3794..4408) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gGAAGACGCT GGAtgcatct 1141 cgggagatag tttgatcagc ttggcgagca cagggaaaag agttcctatt aaggatttgt 1201 taggcgaaaa agattttgaa atatgggcaa ttaatgaaca gacgatgaag ctggaatcag 1261 ctaaagttag tcgtgtattt tgtaccggca aaaagctagt ctatactcta aaaactcgac 1321 taggtagaac tatcaaggca acagcaaatc atagattttt aactattgat ggttggaaaa 1381 gattagatga gctatcttta aaagagcata ttgctctacc ccgtaaacta gaaTAATACT 1441 AGAGttgaga agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg 1501 gacaagaccg gcggtcttaa gttttttggc tgaaTACTAG AGcctttggt cgAAAAAAAA 1561 AGCCCGCACT GTCAGGTGCG GGCTTTTTTc tgtgtttccT ACTAGAGCAA GCGAGCTCGA 1621 TATCAAATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC 1681 TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA 1741 CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA 1801 TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAAACGAAAA 1861 ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT 1921 CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG 1981 AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA 2041 CCAGCTCACC GTCTTTCATT GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA 2101 GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG 2161 CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT 2221 CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT 2281 TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA 2341 GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCTACTA GAGGGACCAA 2401 AACGAAAAAA GACGCTTTTC AGCGTCTTAT TGTTCGTCTT TGGTACCGAG CTCTAGTAGT 2461 GATCTACACT AGCACTATCA GTGTTATTAA TTTTTAAAGT ATGGGCAATC AATTGCTCCT 2521 GTTAAAATTG CTTTAGAAAT ACTTTGGCAG CGGTTTGTTG TATTGAGTTT CATTTGCGCA 2581 TTGGTTAAAT GGAAAGTGAC AGTACGCTCA CTGCAACCTA ATATTTTTGA AATATCCCAA 2641 GAGCTTTTTC CTTCGCATGC CCACGCTAAA CATTCTTTTT CTCTTTTGGT TAAATCGTTG 2701 TTTGATTTAT TATTTGCTAT ATTTATTTTT CGATAATTAT CAACTAGAGA AGGAACAATT 2761 AATGGTATGT TCATACACGC ATGTAAAAAT AAACTATCTA TATAGTTGTC TTTTTCTGAA 2821 TGTGCAAAAC TAAGCATTCC GAAGCCATTG TTAGCCGTAT GAATAGGGAA ACTAAACCCA 2881 GTGATAAGAC CTGATGTTTT CGCTTCTTTA ATTACATTTG GAGATTTTTT ATTTACAGCA 2941 TTGTTTTCAA ATATATTCCA ATTAATTGGT GAATGATTGG AGTTAGAATA ATCTACTATA 3001 GGATCATATT TTATTAAATT AGCGTCATCA TAATATTGCC TCCATTTTTT AGGGTAATTA 3061 TCTAGGATTG AAATATCAGA TTTAACCATA GAATGAGGAT AAATGATCGC GAGTAAATAA 3121 TATTCACAAT GTACCATTTT AGTCATATCA GATAAGCATT GATTAATATC ATTATTGCTT 3181 CTACAAGCTT TAATTTTATT AATTATTCTG TATGTGTCGT CGGCATTTAT GTTTTTCATC 3241 TAGTATTTCT CCTCTTTAAT CTCTAGTAGC TAGCACAATC CCTAGGACTG AGCTAGCTGT 3301 CAATCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATATACTAG ACACCTGTAG 3361 GATCGTACAG GTTTACGCAA GAAAATGGTT TGTTACTTTC GAATAAtact agagtcacac 3421 aggaaagtac tagATGagct cctctttaca attggcacca gaaatagaaa agttgcctca 3481 gagtgatatt tactgggacc ccatcgtttc tattacggag actggagtcg aagaggtttt 3541 tgatttgact gtgccaggac tacgtaactt tgtcgccaat gacatcattg tacataacTC 3601 AAGAAGAGCt actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg 3661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 3721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 3781 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac 3841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 4021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 4081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 4141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 4201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 4261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 4321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 4501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4561 gacagtt //