IBMc050 1 pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI ds-DNA pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI Plasmid carrying Golden Gate substitution insert for IBM, M86 intein, using Plux2 as C-lobe promoter Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin / Chloramphenicol 09-OCT-2020 Baojun Wang Trevor Y. H. Ho annotation8 1 BBa_B0030 #c7b0e3 #c7b0e3 BBa_B0030 range 1 3246 3260 annotation12 1 ampR (rev) #faac61 #faac61 ampR (rev) range 1 1 861 annotation13 1 luxR #b1ff67 #b1ff67 luxR range 1 2484 3239 annotation2 1 M86C #84b0dc #84b0dc M86C range 1 3437 3598 annotation16 1 ECK120029600 #f58a5e #f58a5e ECK120029600 range 1 1445 1534 annotation15 1 VF2 #75c6a9 #75c6a9 VF2 range 1 982 1001 annotation18 1 rep (pMB1) #ff9ccd #ff9ccd rep (pMB1) range 1 3794 4408 annotation11 1 M86N #84b0dc #84b0dc M86N range 1 1134 1433 annotation1 1 RFC10 Suffix #9eafd2 #9eafd2 RFC10 Suffix range 1 3610 3630 annotation6 1 L3S2P55 #ff0000 #ff0000 L3S2P55 range 1 2394 2450 annotation17 1 VR #b4abac #b4abac VR range 1 3688 3707 annotation9 1 BBa_B0012 #c7b0e3 #c7b0e3 BBa_B0012 range 1 3304 3344 annotation14 1 BBa_J23117 #85dae9 #85dae9 BBa_J23117 range 1 3269 3303 annotation3 1 BBa_B0032 #f58a5e #f58a5e BBa_B0032 range 1 3415 3427 annotation10 1 cat (BsmBI-) #ff9ccd #ff9ccd cat (BsmBI-) range 1 1628 2287 annotation0 1 RFC10 Prefix (full) #d6b295 #d6b295 RFC10 Prefix (full) range 1 1100 1121 annotation4 1 Plux2 #75c6a9 #75c6a9 Plux2 range 1 3353 3406 annotation5 1 ECK120033737 #d59687 #d59687 ECK120033737 range 1 1543 1599 annotation7 1 cat promoter #c6c9d1 #c6c9d1 cat promoter range 1 2288 2385 IBMc050_seq 1 accaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactagaaTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCTACTAGAGGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTTATTGTTCGTCTTTGGTACCGAGCTCTAGTAGTGATCTACACTAGCACTATCAGTGTTATTAATTTTTAAAGTATGGGCAATCAATTGCTCCTGTTAAAATTGCTTTAGAAATACTTTGGCAGCGGTTTGTTGTATTGAGTTTCATTTGCGCATTGGTTAAATGGAAAGTGACAGTACGCTCACTGCAACCTAATATTTTTGAAATATCCCAAGAGCTTTTTCCTTCGCATGCCCACGCTAAACATTCTTTTTCTCTTTTGGTTAAATCGTTGTTTGATTTATTATTTGCTATATTTATTTTTCGATAATTATCAACTAGAGAAGGAACAATTAATGGTATGTTCATACACGCATGTAAAAATAAACTATCTATATAGTTGTCTTTTTCTGAATGTGCAAAACTAAGCATTCCGAAGCCATTGTTAGCCGTATGAATAGGGAAACTAAACCCAGTGATAAGACCTGATGTTTTCGCTTCTTTAATTACATTTGGAGATTTTTTATTTACAGCATTGTTTTCAAATATATTCCAATTAATTGGTGAATGATTGGAGTTAGAATAATCTACTATAGGATCATATTTTATTAAATTAGCGTCATCATAATATTGCCTCCATTTTTTAGGGTAATTATCTAGGATTGAAATATCAGATTTAACCATAGAATGAGGATAAATGATCGCGAGTAAATAATATTCACAATGTACCATTTTAGTCATATCAGATAAGCATTGATTAATATCATTATTGCTTCTACAAGCTTTAATTTTATTAATTATTCTGTATGTGTCGTCGGCATTTATGTTTTTCATCTAGTATTTCTCCTCTTTAATCTCTAGTAGCTAGCACAATCCCTAGGACTGAGCTAGCTGTCAATCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGACACCTGTAGGATCGTACAGGTTTACGCAAGAAAATGGTTTGTTACTTTCGAATAAtactagagtcacacaggaaagtactagATGagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtgatatttactgggaccccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgtaactttgtcgccaatgacatcattgtacataacTCAAGAAGAGCtactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagtt