LOCUS IBMc052 5243 bp ds-DNA circular 17-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-T ACCESSION IBMc052 VERSION IBMc052.1 FEATURES Location/Qualifiers RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 3454..3506 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" rep_origin 4088..4912 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 2735..3442 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" misc_feature 3507..3527 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind complement(3622..3641) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 3361 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 3421 cggcatggac gagctgtaca agtaatacta gagaacgcat gagAAAGCCC CCGGAAGATC 3481 ACCTTCCGGG GGCTTTttta ttgcgctact agtagcggcc gctgcaggag tcactaaggg 3541 ttagttagtt agattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact 3601 tcccttgggg ttatcattgg ggctcactca aaggcggtaa tcagataaaa aaaatcctta 3661 gctttcgcta aggatgattt ctgctagaga tggaatagac tggatggagg cggataaagt 3721 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 3781 agccggtgag cgtggatctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 3841 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 3901 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 3961 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 4021 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 4081 agacccctta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc 4141 tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc 4201 tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta 4261 gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg 4321 ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg 4381 cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct 4441 acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca 4501 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg ggaaacgcct 4561 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat 4621 gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggccctc tcacttccct 4681 gttaagtatc ttcctggcat cttccaggaa atctccgccc cgttcgtaag ccatttccgc 4741 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aatatatcct 4801 gtatcacata ttctgctgac gcaccggtgc agcctttttt ctcctgccac atgaagcact 4861 tcactgacac cctcatcagt gccaacatag taagccagta tacactccgc tagcgctgag 4921 gtctgcctcg tgaagaaggt gttgctgact cataccaggc ctgaatcgcc ccatcatcca 4981 gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac cagttggtga 5041 ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc gtgatctgat 5101 ccttcaactc agcaaaagtt cgatttattc aacaaagcca cgttgtgtct caaaatctct 5161 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca 5221 taaacagtaa tacaaggggt gtt //