LOCUS IBMc065 7720 bp ds-DNA circular 17-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD-B30-mCherry*(1-159)-GAG-M86N-cat-Plux2-B32-M86C- S-mCherry*(160-236)-T ACCESSION IBMc065 VERSION IBMc065.1 FEATURES Location/Qualifiers primer_bind complement(6099..6118) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter complement(4375..4472) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" terminator 5931..5983 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(3715..4374) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 5689..5919 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" terminator complement(3630..3686) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5524..5685 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3532..3621 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" RBS 5502..5514 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 3221..3520 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 5440..5493 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" CDS 2735..3211 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator 5391..5431 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" promoter complement(5356..5390) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" RBS complement(5333..5347) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS complement(4571..5326) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" misc_feature 5984..6004 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" rep_origin 6565..7389 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator complement(4481..4537) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgcTGGA tgcatctcgg gagatagttt 3241 gatcagcttg gcgagcacag ggaaaagagt tcctattaag gatttgttag gcgaaaaaga 3301 ttttgaaata tgggcaatta atgaacagac gatgaagctg gaatcagcta aagttagtcg 3361 tgtattttgt accggcaaaa agctagtcta tactctaaaa actcgactag gtagaactat 3421 caaggcaaca gcaaatcata gatttttaac tattgatggt tggaaaagat tagatgagct 3481 atctttaaaa gagcatattg ctctaccccg taaactagaa TAATACTAGA Gttgagaaga 3541 gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg 3601 gtcttaagtt ttttggctga aTACTAGAGc ctttggtcgA AAAAAAAAGC CCGCACTGTC 3661 AGGTGCGGGC TTTTTTctgt gtttccTACT AGAGCAAGCG AGCTCGATAT CAAATTACGC 3721 CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA 3781 GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG 3841 CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT 3901 TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAA ACGAAAAACA TATTCTCAAT 3961 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT 4021 GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT 4081 TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC 4141 TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA 4201 GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG 4261 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT 4321 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC 4381 TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC 4441 ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCTACTAGAG GGACCAAAAC GAAAAAAGAC 4501 GCTTTTCAGC GTCTTATTGT TCGTCTTTGG TACCGAGCTC TAGTAGTGAT CTACACTAGC 4561 ACTATCAGTG TTATTAATTT TTAAAGTATG GGCAATCAAT TGCTCCTGTT AAAATTGCTT 4621 TAGAAATACT TTGGCAGCGG TTTGTTGTAT TGAGTTTCAT TTGCGCATTG GTTAAATGGA 4681 AAGTGACAGT ACGCTCACTG CAACCTAATA TTTTTGAAAT ATCCCAAGAG CTTTTTCCTT 4741 CGCATGCCCA CGCTAAACAT TCTTTTTCTC TTTTGGTTAA ATCGTTGTTT GATTTATTAT 4801 TTGCTATATT TATTTTTCGA TAATTATCAA CTAGAGAAGG AACAATTAAT GGTATGTTCA 4861 TACACGCATG TAAAAATAAA CTATCTATAT AGTTGTCTTT TTCTGAATGT GCAAAACTAA 4921 GCATTCCGAA GCCATTGTTA GCCGTATGAA TAGGGAAACT AAACCCAGTG ATAAGACCTG 4981 ATGTTTTCGC TTCTTTAATT ACATTTGGAG ATTTTTTATT TACAGCATTG TTTTCAAATA 5041 TATTCCAATT AATTGGTGAA TGATTGGAGT TAGAATAATC TACTATAGGA TCATATTTTA 5101 TTAAATTAGC GTCATCATAA TATTGCCTCC ATTTTTTAGG GTAATTATCT AGGATTGAAA 5161 TATCAGATTT AACCATAGAA TGAGGATAAA TGATCGCGAG TAAATAATAT TCACAATGTA 5221 CCATTTTAGT CATATCAGAT AAGCATTGAT TAATATCATT ATTGCTTCTA CAAGCTTTAA 5281 TTTTATTAAT TATTCTGTAT GTGTCGTCGG CATTTATGTT TTTCATCTAG TATTTCTCCT 5341 CTTTAATCTC TAGTAGCTAG CACAATCCCT AGGACTGAGC TAGCTGTCAA TCACACTGGC 5401 TCACCTTCGG GTGGGCCTTT CTGCGTTTAT ATACTAGACA CCTGTAGGAT CGTACAGGTT 5461 TACGCAAGAA AATGGTTTGT TACTTTCGAA TAAtactaga gtcacacagg aaagtactag 5521 ATGagctcct ctttacaatt ggcaccagaa atagaaaagt tgcctcagag tgatatttac 5581 tgggacccca tcgtttctat tacggagact ggagtcgaag aggtttttga tttgactgtg 5641 ccaggactac gtaactttgt cgccaatgac atcattgtac ataacTCAgg cgccctgaag 5701 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag 5761 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 5821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 5881 ggccgccact ccaccggcgg catggacgag ctgtacaagt aatactagag aacgcatgag 5941 AAAGCCCCCG GAAGATCACC TTCCGGGGGC TTTtttattg cgctactagt agcggccgct 6001 gcaggagtca ctaagggtta gttagttaga ttagcagaaa gtcaaaagcc tccgaccgga 6061 ggcttttgac taaaacttcc cttggggtta tcattggggc tcactcaaag gcggtaatca 6121 gataaaaaaa atccttagct ttcgctaagg atgatttctg ctagagatgg aatagactgg 6181 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 6241 attgctgata aatctggagc cggtgagcgt ggatctcgcg gtatcattgc agcactgggg 6301 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 6361 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 6421 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 6481 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 6541 tcgttccact gagcgtcaga ccccttaata agatgatctt cttgagatcg ttttggtctg 6601 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt 6661 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa 6721 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca 6781 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg 6841 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag 6901 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc 6961 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc 7021 cgccagggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga 7081 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gctttgccgc 7141 ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt 7201 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag 7261 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc cttttttctc 7321 ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa gccagtatac 7381 actccgctag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg 7441 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta 7501 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg 7561 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt 7621 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 7681 aaaactgtct gcttacataa acagtaatac aaggggtgtt //