LOCUS IBMc090 3600 bp ds-DNA circular 18-NOV-2020 DEFINITION pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2 ACCESSION IBMc090 VERSION IBMc090.1 FEATURES Location/Qualifiers LTR 971..992 /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="modified R1 for BbsI" CDS 1143..1958 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="KanR" CDS 2826..3485 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="CmR" misc_feature 2630..2632 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="overhang by SapI" LTR complement(2606..2627) /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="modified R1 for SapI" misc_feature 968..968 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="A>G (Glycine)" misc_feature 966..969 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="overhang for BbsI" terminator 1974..2063 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" LTR complement(2584..2605) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="R2" misc_feature complement(2624..2624) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="C>A (SapI)" misc_feature 966..2632 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="BbsI, SapI modified transpsoson" misc_feature complement(2626..2627) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="CG>AA (SapI)" LTR 993..1014 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="R2" terminator complement(2072..2128) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" misc_feature 973..973 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="G>T (BbsI)" CDS complement(2131..2427) /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="Reporter lacZα" rep_origin 183..771 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="ori" promoter complement(2472..2501) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="Plac" misc_feature 975..977 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="GCA>TTC (BbsI)" terminator 3506..3600 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="lambda t0 terminator" ORIGIN 1 ccaagcctcg agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 61 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcctg cataacgcga 961 aGATCTGGAG CGtCttcCGA AAAACGCGAA AGCGTTTCAC GATAAATGCG AAAACTGTCT 1021 GGATCTGAAT AATTAATTAA ATTGTGTCTC AAAATCTCTG ATGTTACATT GCACAAGATA 1081 AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG 1141 TTATGAGCCA TATTCAACGG GAAACGTCTT GTTCGAGGCC GCGATTAAAT TCCAACATGG 1201 ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA 1261 TCTATCGGTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA 1321 GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC 1381 CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG 1441 CGATCCCCGG GAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA 1501 TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC 1561 CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT 1621 TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA 1681 AAGAAATGCA TAAGCTGTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT 1741 CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG 1801 TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT 1861 CTCCTTCATT ACAGGAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA 1921 AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTACTA GAGttgagaa 1981 gagaaaagaa aaccgccgat cctgtccacc gcattactgc aaggtagtgg acaagaccgg 2041 cggtcttaag ttttttggct gaaTACTAGA Gcctttggtc gAAAAAAAAA GCCCGCACTG 2101 TCAGGTGCGG GCTTTTTTct gtgtttccTA CTAgccgcta cagggcgcgt cccattcgcc 2161 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 2221 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 2281 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga 2341 attgggtacc gggccccccc tcgaggtcct ccagcttttg ttccctttag tgagggttaa 2401 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 2461 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 2521 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccaccgg tTGACCCATA 2581 ATGTTTTCGC ATTTATCGTG AAACGCTTTC GCGTTTTTCG TGCtCttCTT CAgatctgaa 2641 attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 2701 gacgtcaagt ggcactcgag ttgatcgggc acgtaagagg ttccaacttt caccataatg 2761 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag 2821 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta 2881 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc 2941 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct 3001 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag 3061 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa 3121 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca 3181 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta 3241 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa 3301 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc 3361 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct 3421 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg 3481 cgtaatttga tatcgagctc gcttggactc ctgttgatag atccagtaat gacctcagaa 3541 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat //