IBMc090 1 pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2 ds-DNA pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2 Plasmid carrying engineered transposon for IBM Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pTHSSd_1 Origin of replication: pMB1 Selection Markers: Kanamycin / Chloramphenicol 18-NOV-2020 Baojun Wang Trevor Y. H. Ho annotation0 1 modified R1 for BbsI #f8d3a9 #f8d3a9 modified R1 for BbsI range 1 971 992 annotation2 1 CmR #b4abac #b4abac CmR range 1 2826 3485 annotation3 1 overhang by SapI #84b0dc #84b0dc overhang by SapI range 1 2630 2632 annotation14 1 G>T (BbsI) #75c6a9 #75c6a9 G>T (BbsI) range 1 973 973 annotation5 1 A>G (Glycine) #75c6a9 #75c6a9 A>G (Glycine) range 1 968 968 annotation12 1 R2 #85dae9 #85dae9 R2 range 1 993 1014 annotation4 1 modified R1 for SapI #f8d3a9 #f8d3a9 modified R1 for SapI range 1 2606 2627 annotation10 1 BbsI, SapI modified transpsoson #b1ff67 #b1ff67 BbsI, SapI modified transpsoson range 1 966 2632 annotation7 1 ECK120029600 #f58a5e #f58a5e ECK120029600 range 1 1974 2063 annotation11 1 CG>AA (SapI) #75c6a9 #75c6a9 CG>AA (SapI) range 1 2626 2627 annotation15 1 Reporter lacZα #84b0dc #84b0dc Reporter lacZα range 1 2131 2427 annotation6 1 overhang for BbsI #84b0dc #84b0dc overhang for BbsI range 1 966 969 annotation18 1 GCA>TTC (BbsI) #75c6a9 #75c6a9 GCA>TTC (BbsI) range 1 975 977 annotation16 1 ori #75c6a9 #75c6a9 ori range 1 183 771 annotation17 1 Plac #faac61 #faac61 Plac range 1 2472 2501 annotation13 1 ECK120033737 #d59687 #d59687 ECK120033737 range 1 2072 2128 annotation9 1 C>A (SapI) #75c6a9 #75c6a9 C>A (SapI) range 1 2624 2624 annotation19 1 lambda t0 terminator #b4abac #b4abac lambda t0 terminator range 1 3506 3600 annotation8 1 R2 #85dae9 #85dae9 R2 range 1 2584 2605 annotation1 1 KanR #b7e6d7 #b7e6d7 KanR range 1 1143 1958 IBMc090_seq 1 ccaagcctcgagctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcctgcataacgcgaaGATCTGGAGCGtCttcCGAAAAACGCGAAAGCGTTTCACGATAAATGCGAAAACTGTCTGGATCTGAATAATTAATTAAATTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCTTGTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGGTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTGTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGGAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaattgggtaccgggccccccctcgaggtcctccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccaccggtTGACCCATAATGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGTGCtCttCTTCAgatctgaaattctgcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaagtggcactcgagttgatcgggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgatatcgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaat