LOCUS       IBMc101                 3244 bp    ds-DNA  circular     17-NOV-2020
DEFINITION  pSB3T5(empty)
ACCESSION   IBMc101
VERSION     IBMc101.1  
FEATURES             Location/Qualifiers
     CDS             816..2009
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /label="tetR"
     primer_bind     complement(2341..2360)
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /label="VR"
     rep_origin      join(1..387,2807..3244)
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /label="p15A origin of replication"
     promoter        727..815
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /label="tetR promoter"
     primer_bind     2089..2108
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /label="VF2"
ORIGIN
        1 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt
       61 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg
      121 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc
      181 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt
      241 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc
      301 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa
      361 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc
      421 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt
      481 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga
      541 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt
      601 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
      661 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttta
      721 ctagaggaga ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat
      781 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg
      841 tcattctcgg caccgtcacc ctggacgctg taggcatagg cttggttatg ccggtactgc
      901 cgggcctctt gcgggatatc gtccattccg acagtattgc cagtcactat ggcgtgctgc
      961 ttgcgctcta tgcgttgatg caatttcttt gcgcacccgt tctcggagcc ctgtccgacc
     1021 gctttggccg ccgtccagtc ctgctcgctt cgctccttgg agccactatc gactacgcga
     1081 tcatggcgac cacacccgtc ctgtggattc tctacgccgg acgcatcgtg gcgggcatca
     1141 cgggtgccac aggtgcggtt gctggtgcct atatcgccga catcaccgat ggggaagatc
     1201 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     1261 tggccggggg actgttgggt gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     1321 tcaacggcct caacctcctc ctgggctgct tccttatgca ggaatcgcat aagggagagc
     1381 gccgtccgat gcccttgcgt gccttcaatc cagtcagctc cttccggtgg gcgcggggca
     1441 tgactatcgt cgccgcactt atgactgttt tctttatcat gcaactcgta ggacaggttc
     1501 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     1561 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcacgggcc
     1621 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc gggcatggcg gccgacgcgc
     1681 tgggctacgt cttgctggcg ttcgcgacgc gcggctggat ggccttcccc attatgattc
     1741 ttctcgcttc cggcggcatc ggtatgcccg cgttgcaggc catgctgtcc cgccaagtag
     1801 atgacgacca tcagggacag cttcaagggt cgctcgcggc tcttaccagc ctcacttcga
     1861 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     1921 tggcatggat tgtaggtgcc gccctttacc ttgtctgcct ccccgcgttg cgtcgcggtg
     1981 catggagccg ggccacctcg acctaataat actagctccg gcaaaaaaac gggcaaggtg
     2041 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg
     2101 tctaagaaaa ggaatattca gcaatttgcc cgtgccgaag aaaggcccac ccgtgaaggt
     2161 gagccagtga gttgattgct acgtaattag ttagttagcc cttagtgact cgaattcgcg
     2221 gccgcttcta gtagcggccg ctgcaggagt cactaagggt tagttagtta gattagcaga
     2281 aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt tatcattggg
     2341 gctcactcaa aggcggtaat cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     2401 tgctagagat ggaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2461 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2521 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2581 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2641 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2701 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc
     2821 ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt
     2881 gcagggcggt ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct
     2941 tggaggagcg cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt
     3001 caagactaac tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc
     3061 tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg
     3121 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     3181 gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga
     3241 acag
//