LOCUS IBMc120 4265 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB4A3-PECF20-B30-mScarlet-I-B15 ACCESSION IBMc120 VERSION IBMc120.1 FEATURES Location/Qualifiers CDS complement(2871..3821) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="repA" misc_feature 1540..1561 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 1562..1621 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="PECF20" terminator 2361..2489 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0015" rep_origin join(complement(1..441),complement(2670..4265)) /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="pSC101 origin" CDS 1651..2349 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2ndVal-mScarlet-I" primer_bind complement(2644..2663) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 2490..2510 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" RBS 1630..1644 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" primer_bind 1422..1441 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" ORIGIN 1 gtgacattca gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca 61 ggcgcctttt atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg 121 gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc 181 agcagttcct gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca 241 ttcagactgg ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta 301 ctgtccctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg 361 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag 421 ctcgtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 721 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 1021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 1081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 1141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 1201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 1261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 1321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 1381 gaaaaataaa caaatagggg ttccgcgcac atttcccatg gtgccacctg acgtctaaga 1441 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag 1501 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga 1561 gGCGCGGATA AAAATTTCAT TTGCCCGCGA CGGATTCCCC GCCCATCTAT CGTTGAACCC 1621 ATACTAGAGA TTAAAGAGGA GAAATACTAG atggtgagta aaggagaagc tgtgattaaa 1681 gagttcatgc gcttcaaagt tcacatggag ggttctatga acggtcacga gttcgagatc 1741 gaaggcgaag gcgagggccg tccgtatgaa ggcacccaga ccgccaaact gaaagtgact 1801 aaaggcggcc cgctgccttt ttcctgggac atcctgagcc cgcaatttat gtacggttct 1861 agggcgttca tcaaacaccc agcggatatc ccggactatt ataagcagtc ttttccggaa 1921 ggtttcaagt gggaacgcgt aatgaatttt gaagatggtg gtgccgtgac cgtcactcag 1981 gacacctccc tggaggatgg caccctgatc tataaagtta aactgcgtgg tactaatttt 2041 ccacctgatg gcccggtgat gcagaaaaag acgatgggtt gggaggcgtc taccgaacgc 2101 ttgtatccgg aagatggtgt gctgaaaggc gacattaaaa tggccctgcg cctgaaagat 2161 ggcggccgct atctggctga cttcaaaacc acgtacaaag ccaagaaacc tgtgcagatg 2221 cctggcgcgt acaatgtgga ccgcaaactg gacatcacct ctcataatga agattatacg 2281 gtggtagagc aatatgagcg ctccgagggt cgtcattcta ccggtggcat ggatgaacta 2341 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 2401 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 2461 accttcgggt gggcctttct gcgtttatat actagtagcg gccgctgcag tccggcaaaa 2521 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt 2581 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 2641 tcagctcact caaaggcggt aatctcgagg ttacattgtc gatctgttca tggtgaacag 2701 ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt 2761 catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt gttctacttt 2821 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt 2881 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac 2941 gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc aaaactggtg 3001 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag 3061 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca 3121 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc 3181 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt 3241 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt 3301 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt 3361 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta 3421 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct 3481 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa 3541 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg 3601 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat 3661 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg 3721 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta 3781 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg 3841 tgattttaat cactatacca attgagatgg gctagtcaat gataattaca tgtccttttc 3901 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct 3961 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg 4021 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct 4081 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg 4141 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc 4201 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct 4261 ttttc //