LOCUS IBMc131 5261 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*-H6-Ter ACCESSION IBMc131 VERSION IBMc131.1 FEATURES Location/Qualifiers CDS 3443..3460 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind complement(3640..3659) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2738..3442 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" terminator 3472..3524 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" misc_feature 3525..3545 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" rep_origin 4106..4930 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 3361 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 3421 cggcatggac gagctgtaca agcatcatca ccatcaccac taatactaga gaacgcatga 3481 gAAAGCCCCC GGAAGATCAC CTTCCGGGGG CTTTtttatt gcgctactag tagcggccgc 3541 tgcaggagtc actaagggtt agttagttag attagcagaa agtcaaaagc ctccgaccgg 3601 aggcttttga ctaaaacttc ccttggggtt atcattgggg ctcactcaaa ggcggtaatc 3661 agataaaaaa aatccttagc tttcgctaag gatgatttct gctagagatg gaatagactg 3721 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 3781 tattgctgat aaatctggag ccggtgagcg tggatctcgc ggtatcattg cagcactggg 3841 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 3901 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 3961 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 4021 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 4081 ttcgttccac tgagcgtcag accccttaat aagatgatct tcttgagatc gttttggtct 4141 gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt 4201 tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa 4261 acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc 4321 aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac 4381 gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca 4441 gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc 4501 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag 4561 ccgccagggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg 4621 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg 4681 cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg 4741 ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga 4801 ggaagcggaa tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct 4861 cctgccacat gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata 4921 cactccgcta gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct 4981 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt 5041 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg 5101 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccacg 5161 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa 5221 taaaactgtc tgcttacata aacagtaata caaggggtgt t //