LOCUS IBMc180 4358 bp ds-DNA circular 09-OCT-2020 DEFINITION pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI ACCESSION IBMc180 VERSION IBMc180.1 FEATURES Location/Qualifiers promoter 2554..2837 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 3006..3362 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" promoter complement(2372..2469) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" rep_origin complement(3585..4199) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" misc_feature 2918..3005 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="BCD22" terminator 2478..2534 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" RBS 2988..2996 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="RBS for 2nd CDS" primer_bind complement(3479..3498) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (rev)" CDS complement(1712..2371) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" misc_feature 3401..3421 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" RBS 2937..2944 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" CDS 1161..1517 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 3363..3392 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2x(GGGGS) linker" misc_feature 1100..1121 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 1529..1618 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 1131..1160 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="2x(GGGGS) linker" misc_feature 2843..2917 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" terminator complement(1627..1683) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gGAAGACGCT GGAGGCGGTG 1141 GTAGCGGTGG TGGCGGTAGC GAAGTTCAGC TGCAAGCAAG CGGTGGTGGT CTGGTTCAGG 1201 CAGGCGGTAG CCTGCGTCTG AGCTGTACCG CAAGCGGTCG TACCGGCACC ATTTATAGCA 1261 TGGCATGGTT TCGTCAGGCA CCGGGTAAAG AACGTGAATT TCTGGCAACC GTTGGTTGGA 1321 GCAGCGGTAT TACCTATTAT ATGGATAGCG TGAAAGGTCG CTTTACCATT AGCCGTGATA 1381 ATGCAAAAAA TAGCGCCTAC CTGCAAATGA ATAGCCTGAA ACCGGAAGAT ACCGCAGTGT 1441 ATTATTGTAC AGCAACCCGT GCATATAGCG TGGGTTATGA TTATTGGGGT CAGGGCACCC 1501 AGGTTACCGT TAGCCATtaa TACTAGAGtt gagaagagaa aagaaaaccg ccgatcctgt 1561 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaTA 1621 CTAGAGcctt tggtcgAAAA AAAAAGCCCG CACTGTCAGG TGCGGGCTTT TTTctgtgtt 1681 tccTACTAGA GCAAGCGAGC TCGATATCAA ATTACGCCCC GCCCTGCCAC TCATCGCAGT 1741 ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA 1801 CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA 1861 AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA 1921 CCCAGGGATT GGCTGAAACG AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA 1981 GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT 2041 CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC 2101 AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT CATTGCCATA CGAAATTCCG 2161 GATGAGCATT CATCAGGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT 2221 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC 2281 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA 2341 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG 2401 ATAACTCAAA AAATACGCCC GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC 2461 TTACGTGCCT ACTAGAGCTC GGTACCAAAG ACGAACAATA AGACGCTGAA AAGCGTCTTT 2521 TTTCGTTTTG GTCCgcttaa cgatcgttgg ctgagaaacc aattgtccat attgcatcag 2581 acattgccgt cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct 2641 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag 2701 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta 2761 tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact 2821 ctctactgtt tctccatacc cgagctgtca ccggatgtgc tttccggtct gatgagtccg 2881 tgaggacgaa acagcctcta caaataattt tgtttaaGGG CCCAAGTTCA CTTAAAAAGG 2941 AGATCAACAA TGAAAGCAAT TTTCGTACTG AAACATCTTA ATCATGCCTA GGAAGTTTTC 3001 TAATGGAAGT TCAGCTGCAA GCAAGCGGTG GTGGTCTGGT TCAGGCAGGC GGTAGCCTGC 3061 GTCTGAGCTG TACCGCAAGC GGTCGTACCG GCACCATTTA TAGCATGGCA TGGTTTCGTC 3121 AGGCACCGGG TAAAGAACGT GAATTTCTGG CAACCGTTGG TTGGAGCAGC GGTATTACCT 3181 ATTATATGGA TAGCGTGAAA GGTCGCTTTA CCATTAGCCG TGATAATGCA AAAAATAGCG 3241 CCTACCTGCA AATGAATAGC CTGAAACCGG AAGATACCGC AGTGTATTAT TGTACAGCAA 3301 CCCGTGCATA TAGCGTGGGT TATGATTATT GGGGTCAGGG CACCCAGGTT ACCGTTAGCC 3361 ATGGTGGAGG TGGTTCAGGT GGCGGAGGTT CAAGAAGAGC tactagtagc ggccgctgca 3421 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 3481 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 3541 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3601 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3781 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3901 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3961 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 4021 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4081 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 4141 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 4201 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 4261 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 4321 aatctaaagt atatatgagt aaacttggtc tgacagtt //