LOCUS IBMc188 7324 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-2aa-acVHH-T-cat-PBAD(Sap I-)-RiboJ-BCD22-acVHH-2aa-ECF20(102-193)-T ACCESSION IBMc188 VERSION IBMc188.1 FEATURES Location/Qualifiers primer_bind complement(5703..5722) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter complement(4251..4348) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 5588..5608 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS complement(3591..4250) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" terminator 5535..5587 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator complement(3506..3562) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5248..5523 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 3408..3497 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 5242..5247 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="GG linker" CDS 3034..3039 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="GG linker" RBS 4867..4875 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="RBS for 2nd CDS" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" RBS 4816..4823 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" misc_feature 4797..4884 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="BCD22" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 4722..4796 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" rep_origin 6169..6993 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 4885..5241 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" promoter 4433..4716 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 3040..3396 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" terminator 4357..4413 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catGGAGGTG AAGTTCAGCT GCAAGCAAGC 3061 GGTGGTGGTC TGGTTCAGGC AGGCGGTAGC CTGCGTCTGA GCTGTACCGC AAGCGGTCGT 3121 ACCGGCACCA TTTATAGCAT GGCATGGTTT CGTCAGGCAC CGGGTAAAGA ACGTGAATTT 3181 CTGGCAACCG TTGGTTGGAG CAGCGGTATT ACCTATTATA TGGATAGCGT GAAAGGTCGC 3241 TTTACCATTA GCCGTGATAA TGCAAAAAAT AGCGCCTACC TGCAAATGAA TAGCCTGAAA 3301 CCGGAAGATA CCGCAGTGTA TTATTGTACA GCAACCCGTG CATATAGCGT GGGTTATGAT 3361 TATTGGGGTC AGGGCACCCA GGTTACCGTT AGCCATtaaT ACTAGAGttg agaagagaaa 3421 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct 3481 taagtttttt ggctgaaTAC TAGAGccttt ggtcgAAAAA AAAAGCCCGC ACTGTCAGGT 3541 GCGGGCTTTT TTctgtgttt ccTACTAGAG CAAGCGAGCT CGATATCAAA TTACGCCCCG 3601 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 3661 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 3721 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 3781 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAAACGA AAAACATATT CTCAATAAAC 3841 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 3901 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 3961 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 4021 ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 4081 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 4141 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 4201 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC 4261 TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA 4321 TGGTGAAAGT TGGAACCTCT TACGTGCCTA CTAGAGCTCG GTACCAAAGA CGAACAATAA 4381 GACGCTGAAA AGCGTCTTTT TTCGTTTTGG TCCgcttaac gatcgttggc tgagaaacca 4441 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggcAc ttctcgctaa 4501 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga 4561 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc 4621 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct 4681 gacgcttttt atcgcaactc tctactgttt ctccataccc gagctgtcac cggatgtgct 4741 ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt gtttaaGGGC 4801 CCAAGTTCAC TTAAAAAGGA GATCAACAAT GAAAGCAATT TTCGTACTGA AACATCTTAA 4861 TCATGCCTAG GAAGTTTTCT AATGGAAGTT CAGCTGCAAG CAAGCGGTGG TGGTCTGGTT 4921 CAGGCAGGCG GTAGCCTGCG TCTGAGCTGT ACCGCAAGCG GTCGTACCGG CACCATTTAT 4981 AGCATGGCAT GGTTTCGTCA GGCACCGGGT AAAGAACGTG AATTTCTGGC AACCGTTGGT 5041 TGGAGCAGCG GTATTACCTA TTATATGGAT AGCGTGAAAG GTCGCTTTAC CATTAGCCGT 5101 GATAATGCAA AAAATAGCGC CTACCTGCAA ATGAATAGCC TGAAACCGGA AGATACCGCA 5161 GTGTATTATT GTACAGCAAC CCGTGCATAT AGCGTGGGTT ATGATTATTG GGGTCAGGGC 5221 ACCCAGGTTA CCGTTAGCCA TGGTTCAgcc tgtgaagcac tggatacccg tccggcaccg 5281 gatgaacagc tggaagcaag cgcacagagc cgtcgtatgg cacaggcact ggatcagctg 5341 ccggatcgtc agcgtgaagc aattgttctg cagtattatc aagaactgag caataccgaa 5401 gcagcagcac tgatgcaaat tagcgttgaa gccctggaaa gcctgctgag ccgtgcacgt 5461 cgtaatctgc gtagccatct ggccgaagca ccgggtgcag atctgagcgg tcgtcgcaaa 5521 ccgtaatact agagaacgca tgagAAAGCC CCCGGAAGAT CACCTTCCGG GGGCTTTttt 5581 attgcgctac tagtagcggc cgctgcagga gtcactaagg gttagttagt tagattagca 5641 gaaagtcaaa agcctccgac cggaggcttt tgactaaaac ttcccttggg gttatcattg 5701 gggctcactc aaaggcggta atcagataaa aaaaatcctt agctttcgct aaggatgatt 5761 tctgctagag atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 5821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggatct 5881 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 5941 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 6001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 6061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 6121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga 6181 tcttcttgag atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc 6241 ttgcagggcg gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg 6301 cttggaggag cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac 6361 ttcaagacta actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg 6421 tctttccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac 6481 ggggggttcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag 6541 gcgtggaatg agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag 6601 gaacaggaga gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt 6661 cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag 6721 cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca 6781 tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc 6841 gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga 6901 cgcaccggtg cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag 6961 tgccaacata gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg 7021 tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 7081 acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 7141 cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 7201 tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga 7261 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg 7321 tgtt //