LOCUS IBMc189 7342 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-5aa-acVHH-T-cat-PBAD(Sap I-)-RiboJ-BCD22-acVHH-5aa-ECF20(102-193)-T ACCESSION IBMc189 VERSION IBMc189.1 FEATURES Location/Qualifiers CDS 5251..5265 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="GGGGS linker" CDS 3034..3048 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="GGGGS linker" RBS 4876..4884 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="RBS for 2nd CDS" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" RBS 4825..4832 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" misc_feature 4806..4893 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="BCD22" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" misc_feature 4731..4805 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" CDS 4894..5250 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" rep_origin 6187..7011 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" promoter 4442..4725 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 3049..3405 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" terminator 4366..4422 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" primer_bind complement(5721..5740) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter complement(4260..4357) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" misc_feature 5606..5626 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS complement(3600..4259) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 5553..5605 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" terminator complement(3515..3571) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5266..5541 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" terminator 3417..3506 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catGGAGGTG GCGGTAGCGA AGTTCAGCTG 3061 CAAGCAAGCG GTGGTGGTCT GGTTCAGGCA GGCGGTAGCC TGCGTCTGAG CTGTACCGCA 3121 AGCGGTCGTA CCGGCACCAT TTATAGCATG GCATGGTTTC GTCAGGCACC GGGTAAAGAA 3181 CGTGAATTTC TGGCAACCGT TGGTTGGAGC AGCGGTATTA CCTATTATAT GGATAGCGTG 3241 AAAGGTCGCT TTACCATTAG CCGTGATAAT GCAAAAAATA GCGCCTACCT GCAAATGAAT 3301 AGCCTGAAAC CGGAAGATAC CGCAGTGTAT TATTGTACAG CAACCCGTGC ATATAGCGTG 3361 GGTTATGATT ATTGGGGTCA GGGCACCCAG GTTACCGTTA GCCATtaaTA CTAGAGttga 3421 gaagagaaaa gaaaaccgcc gatcctgtcc accgcattac tgcaaggtag tggacaagac 3481 cggcggtctt aagttttttg gctgaaTACT AGAGcctttg gtcgAAAAAA AAAGCCCGCA 3541 CTGTCAGGTG CGGGCTTTTT Tctgtgtttc cTACTAGAGC AAGCGAGCTC GATATCAAAT 3601 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 3661 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 3721 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 3781 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAAACGAA AAACATATTC 3841 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 3901 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 3961 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 4021 CCGTCTTTCA TTGCCATACG AAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 4081 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 4141 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 4201 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT 4261 TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT 4321 ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCTAC TAGAGCTCGG TACCAAAGAC 4381 GAACAATAAG ACGCTGAAAA GCGTCTTTTT TCGTTTTGGT CCgcttaacg atcgttggct 4441 gagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggcAct 4501 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 4561 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 4621 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 4681 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg agctgtcacc 4741 ggatgtgctt tccggtctga tgagtccgtg aggacgaaac agcctctaca aataattttg 4801 tttaaGGGCC CAAGTTCACT TAAAAAGGAG ATCAACAATG AAAGCAATTT TCGTACTGAA 4861 ACATCTTAAT CATGCCTAGG AAGTTTTCTA ATGGAAGTTC AGCTGCAAGC AAGCGGTGGT 4921 GGTCTGGTTC AGGCAGGCGG TAGCCTGCGT CTGAGCTGTA CCGCAAGCGG TCGTACCGGC 4981 ACCATTTATA GCATGGCATG GTTTCGTCAG GCACCGGGTA AAGAACGTGA ATTTCTGGCA 5041 ACCGTTGGTT GGAGCAGCGG TATTACCTAT TATATGGATA GCGTGAAAGG TCGCTTTACC 5101 ATTAGCCGTG ATAATGCAAA AAATAGCGCC TACCTGCAAA TGAATAGCCT GAAACCGGAA 5161 GATACCGCAG TGTATTATTG TACAGCAACC CGTGCATATA GCGTGGGTTA TGATTATTGG 5221 GGTCAGGGCA CCCAGGTTAC CGTTAGCCAT GGTGGAGGTG GTTCAgcctg tgaagcactg 5281 gatacccgtc cggcaccgga tgaacagctg gaagcaagcg cacagagccg tcgtatggca 5341 caggcactgg atcagctgcc ggatcgtcag cgtgaagcaa ttgttctgca gtattatcaa 5401 gaactgagca ataccgaagc agcagcactg atgcaaatta gcgttgaagc cctggaaagc 5461 ctgctgagcc gtgcacgtcg taatctgcgt agccatctgg ccgaagcacc gggtgcagat 5521 ctgagcggtc gtcgcaaacc gtaatactag agaacgcatg agAAAGCCCC CGGAAGATCA 5581 CCTTCCGGGG GCTTTtttat tgcgctacta gtagcggccg ctgcaggagt cactaagggt 5641 tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt 5701 cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa aaatccttag 5761 ctttcgctaa ggatgatttc tgctagagat ggaatagact ggatggaggc ggataaagtt 5821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 5881 gccggtgagc gtggatctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 5941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 6001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 6061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 6121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 6181 gaccccttaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct 6241 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct 6301 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag 6361 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc 6421 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc 6481 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta 6541 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac 6601 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg gaaacgcctg 6661 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg 6721 cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctct cacttccctg 6781 ttaagtatct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct 6841 cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg 6901 tatcacatat tctgctgacg caccggtgca gccttttttc tcctgccaca tgaagcactt 6961 cactgacacc ctcatcagtg ccaacatagt aagccagtat acactccgct agcgctgagg 7021 tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag 7081 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat 7141 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc 7201 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg 7261 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 7321 aaacagtaat acaaggggtg tt //