LOCUS IBMc190 7372 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-10aa-acVHH-T-cat-PBAD(Sa pI-)-RiboJ-BCD22-acVHH-10aa-ECF20(102-193)-T ACCESSION IBMc190 VERSION IBMc190.1 FEATURES Location/Qualifiers misc_feature 4746..4820 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" rep_origin 6217..7041 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 4909..5265 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" promoter 4457..4740 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3064..3420 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" terminator 4381..4437 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" primer_bind complement(5751..5770) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter complement(4275..4372) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" misc_feature 5636..5656 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS complement(3615..4274) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" terminator 5583..5635 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" terminator complement(3530..3586) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5296..5571 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 3432..3521 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 5266..5295 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2x(GGGGS) linker" CDS 3034..3063 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="2x(GGGGS) linker" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" RBS 4891..4899 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="RBS for 2nd CDS" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" RBS 4840..4847 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" misc_feature 4821..4908 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="BCD22" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catGGAGGCG GTGGTAGCGG TGGTGGCGGT 3061 AGCGAAGTTC AGCTGCAAGC AAGCGGTGGT GGTCTGGTTC AGGCAGGCGG TAGCCTGCGT 3121 CTGAGCTGTA CCGCAAGCGG TCGTACCGGC ACCATTTATA GCATGGCATG GTTTCGTCAG 3181 GCACCGGGTA AAGAACGTGA ATTTCTGGCA ACCGTTGGTT GGAGCAGCGG TATTACCTAT 3241 TATATGGATA GCGTGAAAGG TCGCTTTACC ATTAGCCGTG ATAATGCAAA AAATAGCGCC 3301 TACCTGCAAA TGAATAGCCT GAAACCGGAA GATACCGCAG TGTATTATTG TACAGCAACC 3361 CGTGCATATA GCGTGGGTTA TGATTATTGG GGTCAGGGCA CCCAGGTTAC CGTTAGCCAT 3421 taaTACTAGA Gttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa 3481 ggtagtggac aagaccggcg gtcttaagtt ttttggctga aTACTAGAGc ctttggtcgA 3541 AAAAAAAAGC CCGCACTGTC AGGTGCGGGC TTTTTTctgt gtttccTACT AGAGCAAGCG 3601 AGCTCGATAT CAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA 3661 AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC 3721 ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG 3781 TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAA 3841 ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC 3901 GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG 3961 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC 4021 CATATCACCA GCTCACCGTC TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG 4081 CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT 4141 AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA 4201 AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG 4261 ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG 4321 CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCTACTAGAG 4381 CTCGGTACCA AAGACGAACA ATAAGACGCT GAAAAGCGTC TTTTTTCGTT TTGGTCCgct 4441 taacgatcgt tggctgagaa accaattgtc catattgcat cagacattgc cgtcactgcg 4501 tcttttactg gcActtctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 4561 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 4621 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 4681 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 4741 acccgagctg tcaccggatg tgctttccgg tctgatgagt ccgtgaggac gaaacagcct 4801 ctacaaataa ttttgtttaa GGGCCCAAGT TCACTTAAAA AGGAGATCAA CAATGAAAGC 4861 AATTTTCGTA CTGAAACATC TTAATCATGC CTAGGAAGTT TTCTAATGGA AGTTCAGCTG 4921 CAAGCAAGCG GTGGTGGTCT GGTTCAGGCA GGCGGTAGCC TGCGTCTGAG CTGTACCGCA 4981 AGCGGTCGTA CCGGCACCAT TTATAGCATG GCATGGTTTC GTCAGGCACC GGGTAAAGAA 5041 CGTGAATTTC TGGCAACCGT TGGTTGGAGC AGCGGTATTA CCTATTATAT GGATAGCGTG 5101 AAAGGTCGCT TTACCATTAG CCGTGATAAT GCAAAAAATA GCGCCTACCT GCAAATGAAT 5161 AGCCTGAAAC CGGAAGATAC CGCAGTGTAT TATTGTACAG CAACCCGTGC ATATAGCGTG 5221 GGTTATGATT ATTGGGGTCA GGGCACCCAG GTTACCGTTA GCCATGGTGG AGGTGGTTCA 5281 GGTGGCGGAG GTTCAgcctg tgaagcactg gatacccgtc cggcaccgga tgaacagctg 5341 gaagcaagcg cacagagccg tcgtatggca caggcactgg atcagctgcc ggatcgtcag 5401 cgtgaagcaa ttgttctgca gtattatcaa gaactgagca ataccgaagc agcagcactg 5461 atgcaaatta gcgttgaagc cctggaaagc ctgctgagcc gtgcacgtcg taatctgcgt 5521 agccatctgg ccgaagcacc gggtgcagat ctgagcggtc gtcgcaaacc gtaatactag 5581 agaacgcatg agAAAGCCCC CGGAAGATCA CCTTCCGGGG GCTTTtttat tgcgctacta 5641 gtagcggccg ctgcaggagt cactaagggt tagttagtta gattagcaga aagtcaaaag 5701 cctccgaccg gaggcttttg actaaaactt cccttggggt tatcattggg gctcactcaa 5761 aggcggtaat cagataaaaa aaatccttag ctttcgctaa ggatgatttc tgctagagat 5821 ggaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 5881 gctggctggt ttattgctga taaatctgga gccggtgagc gtggatctcg cggtatcatt 5941 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 6001 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 6061 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 6121 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 6181 taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc ttcttgagat 6241 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt 6301 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg 6361 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac 6421 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt 6481 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg 6541 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag 6601 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc 6661 gcacgaggga gccgccaggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 6721 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa 6781 cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa 6841 tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag 6901 tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca 6961 gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt 7021 aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg ttgctgactc 7081 ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag 7141 agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc 7201 tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca 7261 acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 7321 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg tt //