LOCUS IBMc191 7402 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-15aa-acVHH-T-cat-PBAD(Sa pI-)-RiboJ-BCD22-acVHH-15aa-ECF20(102-193)-T ACCESSION IBMc191 VERSION IBMc191.1 FEATURES Location/Qualifiers terminator 5613..5665 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" terminator complement(3545..3601) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 5326..5601 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" terminator 3447..3536 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 5281..5325 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="3x(GGGGS) linker" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 3034..3078 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="3x(GGGGS) linker" RBS 4906..4914 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="RBS for 2nd CDS" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" RBS 4855..4862 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" misc_feature 4836..4923 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="BCD22" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 4761..4835 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" rep_origin 6247..7071 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 4924..5280 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" promoter 4472..4755 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 3079..3435 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" terminator 4396..4452 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" primer_bind complement(5781..5800) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter complement(4290..4387) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" misc_feature 5666..5686 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS complement(3630..4289) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catGGAGGTG GCGGTAGCGG AGGCGGTGGT 3061 AGCGGTGGTG GCGGTAGCGA AGTTCAGCTG CAAGCAAGCG GTGGTGGTCT GGTTCAGGCA 3121 GGCGGTAGCC TGCGTCTGAG CTGTACCGCA AGCGGTCGTA CCGGCACCAT TTATAGCATG 3181 GCATGGTTTC GTCAGGCACC GGGTAAAGAA CGTGAATTTC TGGCAACCGT TGGTTGGAGC 3241 AGCGGTATTA CCTATTATAT GGATAGCGTG AAAGGTCGCT TTACCATTAG CCGTGATAAT 3301 GCAAAAAATA GCGCCTACCT GCAAATGAAT AGCCTGAAAC CGGAAGATAC CGCAGTGTAT 3361 TATTGTACAG CAACCCGTGC ATATAGCGTG GGTTATGATT ATTGGGGTCA GGGCACCCAG 3421 GTTACCGTTA GCCATtaaTA CTAGAGttga gaagagaaaa gaaaaccgcc gatcctgtcc 3481 accgcattac tgcaaggtag tggacaagac cggcggtctt aagttttttg gctgaaTACT 3541 AGAGcctttg gtcgAAAAAA AAAGCCCGCA CTGTCAGGTG CGGGCTTTTT Tctgtgtttc 3601 cTACTAGAGC AAGCGAGCTC GATATCAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC 3661 TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAAACGGC ATGATGAACC 3721 TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA 3781 ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC 3841 CAGGGATTGG CTGAAACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG 3901 TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG 3961 TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA 4021 GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG AAATTCCGGA 4081 TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT 4141 TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT 4201 TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG 4261 GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT 4321 AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT 4381 ACGTGCCTAC TAGAGCTCGG TACCAAAGAC GAACAATAAG ACGCTGAAAA GCGTCTTTTT 4441 TCGTTTTGGT CCgcttaacg atcgttggct gagaaaccaa ttgtccatat tgcatcagac 4501 attgccgtca ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta 4561 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg 4621 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg 4681 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct 4741 ctactgtttc tccatacccg agctgtcacc ggatgtgctt tccggtctga tgagtccgtg 4801 aggacgaaac agcctctaca aataattttg tttaaGGGCC CAAGTTCACT TAAAAAGGAG 4861 ATCAACAATG AAAGCAATTT TCGTACTGAA ACATCTTAAT CATGCCTAGG AAGTTTTCTA 4921 ATGGAAGTTC AGCTGCAAGC AAGCGGTGGT GGTCTGGTTC AGGCAGGCGG TAGCCTGCGT 4981 CTGAGCTGTA CCGCAAGCGG TCGTACCGGC ACCATTTATA GCATGGCATG GTTTCGTCAG 5041 GCACCGGGTA AAGAACGTGA ATTTCTGGCA ACCGTTGGTT GGAGCAGCGG TATTACCTAT 5101 TATATGGATA GCGTGAAAGG TCGCTTTACC ATTAGCCGTG ATAATGCAAA AAATAGCGCC 5161 TACCTGCAAA TGAATAGCCT GAAACCGGAA GATACCGCAG TGTATTATTG TACAGCAACC 5221 CGTGCATATA GCGTGGGTTA TGATTATTGG GGTCAGGGCA CCCAGGTTAC CGTTAGCCAT 5281 GGTGGAGGTG GTTCAGGTGG CGGAGGTTCT GGTGGAGGTG GTTCAgcctg tgaagcactg 5341 gatacccgtc cggcaccgga tgaacagctg gaagcaagcg cacagagccg tcgtatggca 5401 caggcactgg atcagctgcc ggatcgtcag cgtgaagcaa ttgttctgca gtattatcaa 5461 gaactgagca ataccgaagc agcagcactg atgcaaatta gcgttgaagc cctggaaagc 5521 ctgctgagcc gtgcacgtcg taatctgcgt agccatctgg ccgaagcacc gggtgcagat 5581 ctgagcggtc gtcgcaaacc gtaatactag agaacgcatg agAAAGCCCC CGGAAGATCA 5641 CCTTCCGGGG GCTTTtttat tgcgctacta gtagcggccg ctgcaggagt cactaagggt 5701 tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt 5761 cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa aaatccttag 5821 ctttcgctaa ggatgatttc tgctagagat ggaatagact ggatggaggc ggataaagtt 5881 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 5941 gccggtgagc gtggatctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 6001 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 6061 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 6121 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 6181 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 6241 gaccccttaa taagatgatc ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct 6301 gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg ttctctgagc taccaactct 6361 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgtcct ttcagtttag 6421 ccttaaccgg cgcatgactt caagactaac tcctctaaat caattaccag tggctgctgc 6481 cagtggtgct tttgcatgtc tttccgggtt ggactcaaga cgatagttac cggataaggc 6541 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta 6601 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac 6661 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg gaaacgcctg 6721 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg 6781 cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc gcggccctct cacttccctg 6841 ttaagtatct tcctggcatc ttccaggaaa tctccgcccc gttcgtaagc catttccgct 6901 cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg 6961 tatcacatat tctgctgacg caccggtgca gccttttttc tcctgccaca tgaagcactt 7021 cactgacacc ctcatcagtg ccaacatagt aagccagtat acactccgct agcgctgagg 7081 tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag 7141 ccagaaagtg agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat 7201 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc 7261 cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc aaaatctctg 7321 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat 7381 aaacagtaat acaaggggtg tt //