LOCUS IBMc226 4743 bp ds-DNA circular 09-OCT-2020 DEFINITION pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter ACCESSION IBMc226 VERSION IBMc226.1 FEATURES Location/Qualifiers CDS 3..863 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (BsaI-)" promoter 1994..2277 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind complement(3114..3133) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 2999..3019 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 2946..2998 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(1088..1966) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" primer_bind 943..962 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" RBS 2286..2313 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="tet1" misc_feature 1066..1087 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 2314..2934 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="TetR" rep_origin 3580..4404 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" ORIGIN 1 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 61 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 121 cacgagtggg ttatatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 181 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 241 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 301 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 361 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 421 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 481 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 541 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 601 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 661 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtGGAt 721 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 781 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 841 cctcactgat taagcattgg taatactagc tccggcaaaa aaacgggcaa ggtgtcacca 901 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct gacgtctaag 961 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca 1021 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct 1081 tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 1141 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 1201 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 1261 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 1321 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 1381 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 1441 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 1501 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 1561 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 1621 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 1681 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 1741 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 1801 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 1861 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 1921 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 1981 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 2041 tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 2101 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 2161 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2221 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac 2281 tagagggaag agagtcaatt cagggtggtg aatatgtcca gattagataa aagtaaagtg 2341 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa 2401 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg 2461 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct 2521 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct 2581 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag 2641 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat 2701 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa 2761 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta 2821 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc 2881 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcctgatac 2941 tagagaacgc atgagAAAGC CCCCGGAAGA TCACCTTCCG GGGGCTTTtt tattgcgcta 3001 ctagtagcgg ccgctgcagg agtcactaag ggttagttag ttagattagc agaaagtcaa 3061 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt ggggctcact 3121 caaaggcggt aatcagataa aaaaaatcct tagctttcgc taaggatgat ttctgctaga 3181 gatggaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 3241 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggatc tcgcggtatc 3301 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 3361 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 3421 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 3481 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 3541 ccttaacgtg agttttcgtt ccactgagcg tcagacccct taataagatg atcttcttga 3601 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc 3661 ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga 3721 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact 3781 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg 3841 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc 3901 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat 3961 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag 4021 agcgcacgag ggagccgcca ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 4081 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa 4141 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg 4201 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc 4261 gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt 4321 gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat 4381 agtaagccag tatacactcc gctagcgctg aggtctgcct cgtgaagaag gtgttgctga 4441 ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc cacggttgat 4501 gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg ccacggaacg 4561 gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgatttat 4621 tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat 4681 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgtttacta 4741 gag //