LOCUS IBMc233 4773 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI ACCESSION IBMc233 VERSION IBMc233.1 FEATURES Location/Qualifiers promoter 2451..2504 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="PlacIQ" terminator 2386..2446 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" misc_feature 3538..3612 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" primer_bind complement(3894..3913) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" terminator 3400..3452 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S3P21" CDS 1134..1433 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" CDS 2533..3135 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="PhlF" primer_bind 982..1001 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" promoter complement(2288..2385) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(1..861) /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="ampR (rev)" RBS 2505..2532 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="phl2" CDS complement(1628..2287) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" RBS 3621..3633 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" misc_feature 3816..3836 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" promoter 3472..3537 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="PPhlF" terminator 3145..3399 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="IOT" terminator 1445..1534 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" terminator complement(1543..1599) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" misc_feature 1100..1121 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 3643..3804 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" rep_origin complement(4000..4614) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="rep (pMB1)" ORIGIN 1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 121 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 301 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 721 gttcgatata acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 841 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 961 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 1021 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 1081 tcgctaagga tgatttctgg aattcgcggc cgcttctaga gGAAGACGCT GGAtgcatct 1141 cgggagatag tttgatcagc ttggcgagca cagggaaaag agttcctatt aaggatttgt 1201 taggcgaaaa agattttgaa atatgggcaa ttaatgaaca gacgatgaag ctggaatcag 1261 ctaaagttag tcgtgtattt tgtaccggca aaaagctagt ctatactcta aaaactcgac 1321 taggtagaac tatcaaggca acagcaaatc atagattttt aactattgat ggttggaaaa 1381 gattagatga gctatcttta aaagagcata ttgctctacc ccgtaaacta gaaTAATACT 1441 AGAGttgaga agagaaaaga aaaccgccga tcctgtccac cgcattactg caaggtagtg 1501 gacaagaccg gcggtcttaa gttttttggc tgaaTACTAG AGcctttggt cgAAAAAAAA 1561 AGCCCGCACT GTCAGGTGCG GGCTTTTTTc tgtgtttccT ACTAGAGCAA GCGAGCTCGA 1621 TATCAAATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC 1681 TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA 1741 CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA 1801 TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAAACGAAAA 1861 ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT 1921 CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG 1981 AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA 2041 CCAGCTCACC GTCTTTCATT GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA 2101 GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG 2161 CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT 2221 CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT 2281 TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA 2341 GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCctcgg taccaaattc 2401 cagaaaagag gcctcccgaa aggggggcct tttttcgttt tggtccaatg gcggcgcgcc 2461 atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc 2521 atgggggtga atatggcacg taccccgagc cgtagcagca ttggtagcct gcgtagtccg 2581 catacccata aagcaattct gaccagcacc attgaaatcc tgaaagaatg tggttatagc 2641 ggtctgagca ttgaaagcgt ggcacgtcgc gccggtgcag gcaaaccgac catttatcgt 2701 tggtggacca acaaagcagc actgattgcc gaagtgtatg aaaatgaaat cgaacaggta 2761 cgtaaatttc cggatttggg tagctttaaa gccgatctgg attttctgct gcataatctg 2821 tggaaagttt ggcgtgaaac catttgtggt gaagcatttc gttgtgttat tgcagaagca 2881 cagttggacc ctgtaaccct gacccaactg aaagatcagt ttatggaacg tcgtcgtgag 2941 ataccgaaaa aactggttga agatgccatt agcaatggtg aactgccgaa agatatcaat 3001 cgtgaactgc tgctggatat gatttttggt ttttgttggt atcgcctgct gaccgaacag 3061 ttgaccgttg aacaggatat tgaagaattt accttcctgc tgattaatgg tgtttgtccg 3121 ggtacacagt gttgataagg atcctaattg gtaacgaatc agacaattga cggctcgagg 3181 gagtagcata gggtttgcag aatccctgct tcgtccattt gacaggcaca ttatgcatcg 3241 atgataagct gtcaaacatg agcagatcct ctacgccgga cgcatcgtgg ccggcatcac 3301 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg 3361 ggctcgccac ttcgggctca tgagcaaata ttttatctgc caattattga aggcctccct 3421 aacggggggc ctttttttgt ttctggtctc ccgcttaacg atcgttggct gcgacgtacg 3481 gtggaatctg attcgttacc aattgacatg atacgaaacg taccgtatcg ttaaggtagc 3541 tgtcaccgga tgtgctttcc ggtctgatga gtccgtgagg acgaaacagc ctctacaaat 3601 aattttgttt aatactagag tcacacagga aagtactagA TGagctcctc tttacaattg 3661 gcaccagaaa tagaaaagtt gcctcagagt gatatttact gggaccccat cgtttctatt 3721 acggagactg gagtcgaaga ggtttttgat ttgactgtgc caggactacg taactttgtc 3781 gccaatgaca tcattgtaca taacTCAAGA AGAGCtacta gtagcggccg ctgcaggctt 3841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 3961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca 4021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 4261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 4321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 4441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 4561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 4621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 4681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 4741 aaagtatata tgagtaaact tggtctgaca gtt //