LOCUS       IBMc249                 2824 bp    ds-DNA  circular     17-NOV-2020
DEFINITION  pSB3A5*(BsaI-)(empty)
ACCESSION   IBMc249
VERSION     IBMc249.1  
FEATURES             Location/Qualifiers
     CDS             174..1034
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /label="ampR (BsaI-)"
     rep_origin      1832..2656
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /label="p15A origin of replication"
     primer_bind     complement(1366..1385)
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /label="VR"
     primer_bind     1114..1133
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /label="VF2"
ORIGIN
        1 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagcca
       61 cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac
      121 aataaaactg tctgcttaca taaacagtaa tacaaggggt gtttactaga ggtatgagta
      181 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      241 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      301 gttatatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      361 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      421 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      481 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      541 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      601 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      661 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
      721 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
      781 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      841 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtGGA tctcgcggta
      901 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      961 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1021 ttaagcattg gtaatactag ctccggcaaa aaaacgggca aggtgtcacc accctgccct
     1081 ttttctttaa aaccgaaaag attacttcgc gtttgccacc tgacgtctaa gaaaaggaat
     1141 attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga
     1201 ttgctacgta attagttagt tagcccttag tgactcgaat tcgcggccgc ttctagtagc
     1261 ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc aaaagcctcc
     1321 gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca ctcaaaggcg
     1381 gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta gagatggaat
     1441 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1501 ctggtttatt gctgataaat ctggagccgg tgagcgtgga tctcgcggta tcattgcagc
     1561 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1621 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1681 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1741 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1801 tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt gagatcgttt
     1861 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
     1921 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc
     1981 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
     2041 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
     2101 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
     2161 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     2221 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
     2281 agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     2341 gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct
     2401 ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc
     2461 gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt
     2521 gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt
     2581 ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc
     2641 agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc
     2701 aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt
     2761 tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
     2821 tgtc
//