LOCUS       IBMc252                 3099 bp    ds-DNA  circular     17-NOV-2020
DEFINITION  pSEVA471(BsbI+)
ACCESSION   IBMc252
VERSION     IBMc252.1  
FEATURES             Location/Qualifiers
     primer_bind     complement(3009..3025)
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /label="PS2"
     rep_origin      2240..2462
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /label="pSC101 ori"
     terminator      complement(2729..2815)
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="rrnB T1 terminator"
     misc_feature    2930..2950
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /label="RFC10 Suffix"
     CDS             complement(1242..2192)
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
                     /label="Rep101"
     terminator      2955..3049
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="lambda t0 terminator"
     primer_bind     complement(3009..3025)
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /label="pSEVA PS2 Rev"
     CDS             186..977
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /label="SmR"
     misc_feature    2877..2898
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /label="RFC10 Prefix (full)"
     primer_bind     2719..2736
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /label="pSEVA PS1 Fwd"
ORIGIN
        1 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
       61 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
      121 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      181 acatcATGAG GGAAGCGGTG ATCGCCGAAG TATCGACTCA ACTATCAGAG GTAGTTGGCG
      241 TCATCGAGCG CCATCTCGAA CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG
      301 ATGGCGGCCT GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG
      361 ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT TCCCCTGGAG
      421 AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT GCACGACGAC ATCATTCCGT
      481 GGCGTTATCC AGCTAAGCGC GAACTGCAAT TTGGAGAATG GCAGCGCAAT GACATTCTTG
      541 CAGGTATCTT CGAGCCAGCC ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA
      601 GAGAACATAG CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG
      661 AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG CCGCCCGACT
      721 GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG CATTTGGTAC AGCGCAGTAA
      781 CCGGCAAAAT CGCGCCGAAG GATGTCGCTG CCGACTGGGC AATGGAGCGC CTGCCGGCCC
      841 AGTATCAGCC CGTCATACTT GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT
      901 TGGCCTCGCG CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA
      961 AGGTAGTCGG CAAATAAGAC aaTTGTCCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT
     1021 TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG
     1081 GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT
     1141 AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT
     1201 CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GTAGGCCGGC CTCAGATCCT TCCGTATTTA
     1261 GCCAGTATGT TCTCTAGTGT GGTTCGTTGT TTTTGCGTGA GCCATGAGAA CGAACCATTG
     1321 AGATCATACT TACTTTGCAT GTCACTCAAA AATTTTGCCT CAAAACTGGT GAGCTGAATT
     1381 TTTGCAGTTA AAGCATCGTG TAGTGTTTTT CTTAGTCCGT TATGTAGGTA GGAATCTGAT
     1441 GTAATGGTTG TTGGTATTTT GTCACCATTC ATTTTTATCT GGTTGTTCTC AAGTTCGGTT
     1501 ACGAGATCCA TTTGTCTATC TAGTTCAACT TGGAAAATCA ACGTATCAGT CGGGCGGCCT
     1561 CGCTTATCAA CCACCAATTT CATATTGCTG TAAGTGTTTA AATCTTTACT TATTGGTTTC
     1621 AAAACCCATT GGTTAAGCCT TTTAAACTCA TGGTAGTTAT TTTCAAGCAT TAACATGAAC
     1681 TTAAATTCAT CAAGGCTAAT CTCTATATTT GCCTTGTGAG TTTTCTTTTG TGTTAGTTCT
     1741 TTTAATAACC ACTCATAAAT CCTCATAGAG TATTTGTTTT CAAAAGACTT AACATGTTCC
     1801 AGATTATATT TTATGAATTT TTTTAACTGG AAAAGATAAG GCAATATCTC TTCACTAAAA
     1861 ACTAATTCTA ATTTTTCGCT TGAGAACTTG GCATAGTTTG TCCACTGGAA AATCTCAAAG
     1921 CCTTTAACCA AAGGATTCCT GATTTCCACA GTTCTCGTCA TCAGCTCTCT GGTTGCTTTA
     1981 GCTAATACAC CATAAGCATT TTCCCTACTG ATGTTCATCA TCTGAGCGTA TTGGTTATAA
     2041 GTGAACGATA CCGTCCGTTC TTTCCTTGTA GGGTTTTCAA TCGTGGGGTT GAGTAGTGCC
     2101 ACACAGCATA AAATTAGCTT GGTTTCATGC TCCGTTAAGT CATAGCGACT AATCGCTAGT
     2161 TCATTTGCTT TGAAAACAAC TAATTCAGAC ATACATCTCA ATTGGTCTAG GTGATTTTAA
     2221 TCACTATACC AATTGAGATG GGCTAGTCAA TGATAATTAC ATGTCCTTTT CCTTTGAGTT
     2281 GTGGGTATCT GTAAATTCTG CTAGACCTTT GCTGGAAAAC TTGTAAATTC TGCTAGACCC
     2341 TCTGTAAATT CCGCTAGACC TTTGTGTGTT TTTTTTGTTT ATATTCAAGT GGTTATAATT
     2401 TATAGAATAA AGAAAGAATA AAAAAAGATA AAAAGAATAG ATCCCAGCCC TGTGTATAAC
     2461 TCACTACTTT AGTCAGTTCC GCAGTATTAC AAAAGGATGT CGCAAACGCT GTTTGCTCCT
     2521 CTACAAAACA GACCTTAAAA CCCTAAAGGC TTAAGTAGCA CCCTCGCAAG CTCGGGCAAA
     2581 TCGCTGAATA TTCCTTTTGT CTCCGACCAT CAGGCACCTG AGTCGCTGTC TTTTTCGTGA
     2641 CATTCAGTTC GCTGCGCTCA CGGCTCTGGC AGTGAATGGG GGTAAATGGC ACTACAGGCG
     2701 CGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA
     2761 AACAACAGAT AAAACGAAAG GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC
     2821 TTTAATTAAA GCGGATAACA ATTTCACACA GGAGGCCGCC TAGGCCGCGG CCGCGCGAAT
     2881 TCGcggccgc ttctagaggt acgcGTCTTC ggttagGAAG ACgcagctgt actagtagcg
     2941 gccgctgcag CTTGGACTCC TGTTGATAGA TCCAGTAATG ACCTCAGAAC TCCATCTGGA
     3001 TTTGTTCAGA ACGCTCGGTT GCCGCCGGGC GTTTTTTATT GGTGAGAATC CAGGGGTCCC
     3061 CAATAATTAC GATTTACGTA TTTAAATgaa ccttgaccg
//