LOCUS IBMc259 4024 bp ds-DNA circular 09-OCT-2020 DEFINITION pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter ACCESSION IBMc259 VERSION IBMc259.1 FEATURES Location/Qualifiers CDS 3095..3790 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="mScarlet-I" promoter 2918..2993 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="pTet*" rep_origin 2240..2462 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="pSC101 ori" CDS complement(1242..2192) /ApEinfo_fwdcolor="#993366" /ApEinfo_revcolor="#993366" /label="Rep101" CDS 186..977 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="SmR" terminator 3880..3974 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" RBS 3077..3088 /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="BBa_B0064" primer_bind 2719..2736 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" misc_feature 2877..2898 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" terminator 3794..3854 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="L3S2P21" misc_feature 2994..3068 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RiboJ10" /featureType="insulator" primer_bind complement(3934..3950) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" terminator complement(2729..2815) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="rrnB T1 terminator" misc_feature 3855..3875 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" ORIGIN 1 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg 61 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt 121 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa 181 acatcATGAG GGAAGCGGTG ATCGCCGAAG TATCGACTCA ACTATCAGAG GTAGTTGGCG 241 TCATCGAGCG CCATCTCGAA CCGACGTTGC TGGCCGTACA TTTGTACGGC TCCGCAGTGG 301 ATGGCGGCCT GAAGCCACAC AGTGATATTG ATTTGCTGGT TACGGTGACC GTAAGGCTTG 361 ATGAAACAAC GCGGCGAGCT TTGATCAACG ACCTTTTGGA AACTTCGGCT TCCCCTGGAG 421 AGAGCGAGAT TCTCCGCGCT GTAGAAGTCA CCATTGTTGT GCACGACGAC ATCATTCCGT 481 GGCGTTATCC AGCTAAGCGC GAACTGCAAT TTGGAGAATG GCAGCGCAAT GACATTCTTG 541 CAGGTATCTT CGAGCCAGCC ACGATCGACA TTGATCTGGC TATCTTGCTG ACAAAAGCAA 601 GAGAACATAG CGTTGCCTTG GTAGGTCCAG CGGCGGAGGA ACTCTTTGAT CCGGTTCCTG 661 AACAGGATCT ATTTGAGGCG CTAAATGAAA CCTTAACGCT ATGGAACTCG CCGCCCGACT 721 GGGCTGGCGA TGAGCGAAAT GTAGTGCTTA CGTTGTCCCG CATTTGGTAC AGCGCAGTAA 781 CCGGCAAAAT CGCGCCGAAG GATGTCGCTG CCGACTGGGC AATGGAGCGC CTGCCGGCCC 841 AGTATCAGCC CGTCATACTT GAAGCTAGAC AGGCTTATCT TGGACAAGAA GAAGATCGCT 901 TGGCCTCGCG CGCAGATCAG TTGGAAGAAT TTGTCCACTA CGTGAAAGGC GAGATCACCA 961 AGGTAGTCGG CAAATAAGAC aaTTGTCCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT 1021 TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG 1081 GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT 1141 AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT 1201 CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GTAGGCCGGC CTCAGATCCT TCCGTATTTA 1261 GCCAGTATGT TCTCTAGTGT GGTTCGTTGT TTTTGCGTGA GCCATGAGAA CGAACCATTG 1321 AGATCATACT TACTTTGCAT GTCACTCAAA AATTTTGCCT CAAAACTGGT GAGCTGAATT 1381 TTTGCAGTTA AAGCATCGTG TAGTGTTTTT CTTAGTCCGT TATGTAGGTA GGAATCTGAT 1441 GTAATGGTTG TTGGTATTTT GTCACCATTC ATTTTTATCT GGTTGTTCTC AAGTTCGGTT 1501 ACGAGATCCA TTTGTCTATC TAGTTCAACT TGGAAAATCA ACGTATCAGT CGGGCGGCCT 1561 CGCTTATCAA CCACCAATTT CATATTGCTG TAAGTGTTTA AATCTTTACT TATTGGTTTC 1621 AAAACCCATT GGTTAAGCCT TTTAAACTCA TGGTAGTTAT TTTCAAGCAT TAACATGAAC 1681 TTAAATTCAT CAAGGCTAAT CTCTATATTT GCCTTGTGAG TTTTCTTTTG TGTTAGTTCT 1741 TTTAATAACC ACTCATAAAT CCTCATAGAG TATTTGTTTT CAAAAGACTT AACATGTTCC 1801 AGATTATATT TTATGAATTT TTTTAACTGG AAAAGATAAG GCAATATCTC TTCACTAAAA 1861 ACTAATTCTA ATTTTTCGCT TGAGAACTTG GCATAGTTTG TCCACTGGAA AATCTCAAAG 1921 CCTTTAACCA AAGGATTCCT GATTTCCACA GTTCTCGTCA TCAGCTCTCT GGTTGCTTTA 1981 GCTAATACAC CATAAGCATT TTCCCTACTG ATGTTCATCA TCTGAGCGTA TTGGTTATAA 2041 GTGAACGATA CCGTCCGTTC TTTCCTTGTA GGGTTTTCAA TCGTGGGGTT GAGTAGTGCC 2101 ACACAGCATA AAATTAGCTT GGTTTCATGC TCCGTTAAGT CATAGCGACT AATCGCTAGT 2161 TCATTTGCTT TGAAAACAAC TAATTCAGAC ATACATCTCA ATTGGTCTAG GTGATTTTAA 2221 TCACTATACC AATTGAGATG GGCTAGTCAA TGATAATTAC ATGTCCTTTT CCTTTGAGTT 2281 GTGGGTATCT GTAAATTCTG CTAGACCTTT GCTGGAAAAC TTGTAAATTC TGCTAGACCC 2341 TCTGTAAATT CCGCTAGACC TTTGTGTGTT TTTTTTGTTT ATATTCAAGT GGTTATAATT 2401 TATAGAATAA AGAAAGAATA AAAAAAGATA AAAAGAATAG ATCCCAGCCC TGTGTATAAC 2461 TCACTACTTT AGTCAGTTCC GCAGTATTAC AAAAGGATGT CGCAAACGCT GTTTGCTCCT 2521 CTACAAAACA GACCTTAAAA CCCTAAAGGC TTAAGTAGCA CCCTCGCAAG CTCGGGCAAA 2581 TCGCTGAATA TTCCTTTTGT CTCCGACCAT CAGGCACCTG AGTCGCTGTC TTTTTCGTGA 2641 CATTCAGTTC GCTGCGCTCA CGGCTCTGGC AGTGAATGGG GGTAAATGGC ACTACAGGCG 2701 CGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT CAGGAGAGCG TTCACCGACA 2761 AACAACAGAT AAAACGAAAG GCCCAGTCTT TCGACTGAGC CTTTCGTTTT ATTTGATGCC 2821 TTTAATTAAA GCGGATAACA ATTTCACACA GGAGGCCGCC TAGGCCGCGG CCGCGCGaat 2881 tcgcggccgc ttctagaggc ttaacgatcg ttggctgttt tcagcaggac gcactgacct 2941 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacagcgctc 3001 aacgggtgtg cttcccgttc tgatgagtcc gtgaggacga aagcgcctct acaaataatt 3061 ttgtttaata ctagagaaag aggggaaata ctagatggtg agtaaaggag aagctgtgat 3121 taaagagttc atgcgcttca aagttcacat ggagggttct atgaacggtc acgagttcga 3181 gatcgaaggc gaaggcgagg gccgtccgta tgaaggcacc cagaccgcca aactgaaagt 3241 gactaaaggc ggcccgctgc ctttttcctg ggacatcctg agcccgcaat ttatgtacgg 3301 ttctagggcg ttcatcaaac acccagcgga tatcccggac tattataagc agtcttttcc 3361 ggaaggtttc aagtgggaac gcgtaatgaa ttttgaagat ggtggtgccg tgaccgtcac 3421 tcaggacacc tccctggagg atggcaccct gatctataaa gttaaactgc gtggtactaa 3481 ttttccacct gatggcccgg tgatgcagaa aaagacgatg ggttgggagg cgtctaccga 3541 acgcttgtat ccggaagatg gtgtgctgaa aggcgacatt aaaatggccc tgcgcctgaa 3601 agatggcggc cgctatctgg ctgacttcaa aaccacgtac aaagccaaga aacctgtgca 3661 gatgcctggc gcgtacaatg tggaccgcaa actggacatc acctctcata atgaagatta 3721 tacggtggta gagcaatatg agcgctccga gggtcgtcat tctaccggtg gcatggatga 3781 actatacaaa taactcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt 3841 tttcgttttg gtcctactag tagcggccgc tgcagCTTGG ACTCCTGTTG ATAGATCCAG 3901 TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC CGGGCGTTTT 3961 TTATTGGTGA GAATCCAGGG GTCCCCAATA ATTACGATTT ACGTATTTAA ATgaaccttg 4021 accg //