IBMc259 1 pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter ds-DNA pSEVA471-PTet*-RiboJ10-B64-mScarlet-I-Ter TetR reporter plasmid Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA471 Origin of replication: pSC101 Selection Markers: Spectinomycin 09-OCT-2020 Trevor Y. H. Ho Baojun Wang annotation7 1 pSEVA PS1 Fwd #85dae9 #85dae9 pSEVA PS1 Fwd range 1 2719 2736 annotation11 1 pSEVA PS2 Rev #85dae9 #85dae9 pSEVA PS2 Rev range 1 3934 3950 annotation10 1 RiboJ10 #9eafd2 #9eafd2 RiboJ10 insulator range 1 2994 3068 annotation8 1 RFC10 Prefix (full) #d6b295 #d6b295 RFC10 Prefix (full) range 1 2877 2898 annotation0 1 mScarlet-I #ff9ccd #ff9ccd mScarlet-I range 1 3095 3790 annotation2 1 pSC101 ori #ffef86 #ffef86 pSC101 ori range 1 2240 2462 annotation4 1 SmR #b7e6d7 #b7e6d7 SmR range 1 186 977 annotation13 1 RFC10 Suffix #9eafd2 #9eafd2 RFC10 Suffix range 1 3855 3875 annotation12 1 rrnB T1 terminator #c6c9d1 #c6c9d1 rrnB T1 terminator range 1 2729 2815 annotation6 1 BBa_B0064 #f8d3a9 #f8d3a9 BBa_B0064 range 1 3077 3088 annotation9 1 L3S2P21 #84b0dc #84b0dc L3S2P21 range 1 3794 3854 annotation1 1 pTet* #f58a5e #f58a5e pTet* range 1 2918 2993 annotation5 1 lambda t0 terminator #c6c9d1 #c6c9d1 lambda t0 terminator range 1 3880 3974 annotation3 1 Rep101 #993366 #993366 Rep101 range 1 1242 2192 IBMc259_seq 1 aacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACaaTTGTCCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAAGCGGATAACAATTTCACACAGGAGGCCGCCTAGGCCGCGGCCGCGCGaattcgcggccgcttctagaggcttaacgatcgttggctgttttcagcaggacgcactgacctccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacagcgctcaacgggtgtgcttcccgttctgatgagtccgtgaggacgaaagcgcctctacaaataattttgtttaatactagagaaagaggggaaatactagatggtgagtaaaggagaagctgtgattaaagagttcatgcgcttcaaagttcacatggagggttctatgaacggtcacgagttcgagatcgaaggcgaaggcgagggccgtccgtatgaaggcacccagaccgccaaactgaaagtgactaaaggcggcccgctgcctttttcctgggacatcctgagcccgcaatttatgtacggttctagggcgttcatcaaacacccagcggatatcccggactattataagcagtcttttccggaaggtttcaagtgggaacgcgtaatgaattttgaagatggtggtgccgtgaccgtcactcaggacacctccctggaggatggcaccctgatctataaagttaaactgcgtggtactaattttccacctgatggcccggtgatgcagaaaaagacgatgggttgggaggcgtctaccgaacgcttgtatccggaagatggtgtgctgaaaggcgacattaaaatggccctgcgcctgaaagatggcggccgctatctggctgacttcaaaaccacgtacaaagccaagaaacctgtgcagatgcctggcgcgtacaatgtggaccgcaaactggacatcacctctcataatgaagattatacggtggtagagcaatatgagcgctccgagggtcgtcattctaccggtggcatggatgaactatacaaataactcggtaccaaattccagaaaagaggcctcccgaaaggggggccttttttcgttttggtcctactagtagcggccgctgcagCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATgaaccttgaccg