LOCUS IBMc307 5729 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86-S-mCherry*(193- 236)-H6-T ACCESSION IBMc307 VERSION IBMc307.1 FEATURES Location/Qualifiers primer_bind complement(4108..4127) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 3314..3775 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86" CDS 2735..3310 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 3911..3928 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 3940..3992 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" rep_origin 4574..5398 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS 3779..3910 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccgtg GGAtgcatct cgggagatag tttgatcagc ttggcgagca cagggaaaag 3361 agttcctatt aaggatttgt taggcgaaaa agattttgaa atatgggcaa ttaatgaaca 3421 gacgatgaag ctggaatcag ctaaagttag tcgtgtattt tgtaccggca aaaagctagt 3481 ctatactcta aaaactcgac taggtagaac tatcaaggca acagcaaatc atagattttt 3541 aactattgat ggttggaaaa gattagatga gctatcttta aaagagcata ttgctctacc 3601 ccgtaaacta gaaagctcct ctttacaatt ggcaccagaa atagaaaagt tgcctcagag 3661 tgatatttac tgggacccca tcgtttctat tacggagact ggagtcgaag aggtttttga 3721 tttgactgtg ccaggactac gtaactttgt cgccaatgac atcattgtac ataacTCAca 3781 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta 3841 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga 3901 gctgtacaag catcatcacc atcaccacta atactagaga acgcatgagA AAGCCCCCGG 3961 AAGATCACCT TCCGGGGGCT TTtttattgc gctactagta gcggccgctg caggagtcac 4021 taagggttag ttagttagat tagcagaaag tcaaaagcct ccgaccggag gcttttgact 4081 aaaacttccc ttggggttat cattggggct cactcaaagg cggtaatcag ataaaaaaaa 4141 tccttagctt tcgctaagga tgatttctgc tagagatgga atagactgga tggaggcgga 4201 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 4261 atctggagcc ggtgagcgtg gatctcgcgg tatcattgca gcactggggc cagatggtaa 4321 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 4381 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 4441 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 4501 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 4561 agcgtcagac cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc 4621 ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac 4681 caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc 4741 agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg 4801 ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg 4861 ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa 4921 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga 4981 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggaa 5041 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt 5101 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac 5161 ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat 5221 ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata 5281 tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga 5341 agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc 5401 gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat 5461 catccagcca gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt 5521 tggtgatttt gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga 5581 tctgatcctt caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa 5641 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg 5701 cttacataaa cagtaataca aggggtgtt //