IBMc318 1 pSB1A3-BbsI-G-ER-LBD-S-SapI ds-DNA pSB1A3-BbsI-G-ER-LBD-S-SapI Plasmid carrying Golden Gate substitution insert for ER-LBD domain-insertion mapping Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB1A3 Origin of replication: pMB1 Selection Markers: Ampicillin 18-NOV-2020 Trevor Y. H. Ho Baojun Wang annotation3 1 ampR (rev) #faac61 #faac61 ampR (rev) range 1 59 919 annotation2 1 VF2 #75c6a9 #75c6a9 VF2 range 1 1040 1059 annotation1 1 ER-LBD #ffef86 #ffef86 ER-LBD range 1 1192 1935 annotation6 1 rep (pMB1) #ff9ccd #ff9ccd rep (pMB1) range 1 2131 2745 annotation5 1 RFC10 Suffix #9eafd2 #9eafd2 RFC10 Suffix range 1 1947 1967 annotation4 1 RFC10 Prefix (full) #d6b295 #d6b295 RFC10 Prefix (full) range 1 1158 1179 annotation0 1 VR #b4abac #b4abac VR range 1 2025 2044 IBMc318_seq 1 taaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatataacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattcgcggccgcttctagagGAAGACGCTGGAAATAGCCTGGCACTGAGCCTGACCGCAGATCAGATGGTTAGCGCACTGCTGGATGCAGAACCGCCTATTCTGTATAGCGAATATGATCCGACACGTCCGTTTAGCGAAGCAAGCATGATGGGTCTGCTGACCAATCTGGCAGATCGTGAACTGGTTCATATGATTAATTGGGCAAAACGTGTTCCGGGTTTTGTTGATCTGACCCTGCATGATCAGGTTCATCTGCTGGAATGTGCATGGCTGGAAATTCTGATGATTGGTCTGGTTTGGCGTAGCATGGAACATCCGGGTAAACTGCTGTTTGCACCGAATCTGCTGCTGGATCGTAATCAGGGTAAATGTGTTGAAGGTATGGTGGAAATCTTTGATATGCTGCTGGCAACCAGCAGCCGTTTTCGTATGATGAATCTGCAAGGTGAAGAATTTGTTTGCCTGAAAAGCATTATTCTGCTGAACAGCGGTGTTTATACCTTTCTGAGCAGCACCCTGAAAAGTCTGGAAGAAAAAGATCATATCCATCGTGTGCTGGATAAAATCACCGATACACTGATTCATCTGATGGCAAAAGCCGGTCTGACCTTACAGCAGCAGCATCAGCGTCTGGCACAACTGCTGCTGATTCTGAGCCATATTCGTCATATGAGCAATAAAGGTATGGAACACCTGTACAGCATGAAATGCAAAAATGTTGTTCCGCTGTATGACCTGCTGTTAGAAATGCTGGACGCACATCGTCTGCATGCATCAAGAAGAGCtactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaat