LOCUS IBMc325 6506 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-37R3-2-S-mCherry*(1 93-236)-H6-Ter ACCESSION IBMc325 VERSION IBMc325.1 FEATURES Location/Qualifiers primer_bind complement(4885..4904) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 4556..4687 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" CDS 3314..4552 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Mtu RecA 37R3-2" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" terminator 4717..4769 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 4770..4790 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" rep_origin 5351..6175 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 4688..4705 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 2735..3310 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccGTT GGATGTCTGG CAGAAGGCAC CCGTATTTTT GATCCGGTTA CCGGTACAAC 3361 CCATCGTATT GAAGATGTTG TTGATGGTCG TAAACCGATT CATGTTGTTG CAGCAGCAAA 3421 AGATGGCACC CTGCTGGCAC GTCCGGTTGT TAGCTGGTTT GATCAGGGCA CCCGTGATGT 3481 TATTGGTCTG CGTATTGCCG GTGGTGCAAT TGTTTGGGCA ACACCGGATC ATAAAGTTCT 3541 GACCGAATAT GGTTGGCGTG CAGCCGGTGA ACTGCGTAAA GGTGATCGTG TTGCAGGTCC 3601 TGGTGGTAGC GGTAATAGCC TGGCACTGAG CCTGACCGCA GATCAGATGG TTAGCGCACT 3661 GCTGGATGCA GAACCGCCTA TTCTGTATAG CGAATATGAT CCGACCAGTC CGTTTAGCGA 3721 AGCAAGCATG ATGGGTCTGC TGACCAATCT GGCAGATCGT GAACTGGTTC ATATGATTAA 3781 TTGGGCAAAA CGTGTTCCGG GTTTTGTTGA TCTGACCCTG CATGATCAGG CACATCTGCT 3841 GGAACGTGCA TGGCTGGAAA TTCTGATGAT TGGTCTGGTT TGGCGTAGCA TGGAACATCC 3901 GGGTAAACTG CTGTTTGCAC CGAATCTGCT GCTGGATCGT AATCAGGGTA AATGTGTTGA 3961 AGGTATGGTG GAAATCTTTG ATATGCTGCT GGCAACCAGC AGCCGTTTTC GTATGATGAA 4021 TCTGCAAGGT GAAGAATTTG TTTGCCTGAA AAGCATTATT CTGCTGAACA GCGGTGTTTA 4081 TACCTTTCTG AGCAGCACCC TGAAAAGTCT GGAAGAAAAA GATCATATCC ATCGTGCCCT 4141 GGATAAAATC ACCGATACAC TGATTCATCT GATGGCAAAA GCCGGTCTGA CCTTACAGCA 4201 GCAGCATCAG CGTCTGGCAC AACTGCTGCT GATTCTGAGC CATATTCGTC ATATGAGCAA 4261 TAAAGGTATG GAACACCTGT ACAGCATGAA GTATAAAAAC GTTGTGCCGC TGTATGATCT 4321 GCTGTTAGAA ATGCTGGACG CACATCGTCT GCATGCCGGT GGCAGCGGTG CAAGCCGTGT 4381 TCAGGCATTT GCAGATGCAC TGGATGATAA ATTTCTGCAC GATATGCTGG CCGAAGGTCT 4441 GCGTTATAGC GTTATTCGTG AAGTTCTGCC GACACGTCGT GCACGTACCT TTGATCTGGA 4501 AGTTGAAGAA CTGCATACCC TGGTTGCAGA AGGTGTTGTT GTTCATAATT GCTCAcagct 4561 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 4621 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 4681 gtacaagcat catcaccatc accactaata ctagagaacg catgagAAAG CCCCCGGAAG 4741 ATCACCTTCC GGGGGCTTTt ttattgcgct actagtagcg gccgctgcag gagtcactaa 4801 gggttagtta gttagattag cagaaagtca aaagcctccg accggaggct tttgactaaa 4861 acttcccttg gggttatcat tggggctcac tcaaaggcgg taatcagata aaaaaaatcc 4921 ttagctttcg ctaaggatga tttctgctag agatggaata gactggatgg aggcggataa 4981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 5041 tggagccggt gagcgtggat ctcgcggtat cattgcagca ctggggccag atggtaagcc 5101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 5161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 5221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 5281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 5341 gtcagacccc ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg 5401 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa 5461 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt 5521 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg 5581 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata 5641 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg 5701 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg 5761 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc aggggaaacg 5821 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt 5881 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc 5941 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc 6001 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat 6061 cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc 6121 acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct 6181 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat 6241 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg 6301 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct 6361 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc 6421 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt 6481 acataaacag taatacaagg ggtgtt //