IBMc330 1 pSEVA171-PSrpR-B30-mScarlet-I-Ter ds-DNA pSEVA171-PSrpR-B30-mScarlet-I-Ter SrpR reporter plasmid Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSEVA171 Origin of replication: pSC101 Selection Markers: Ampicillin 18-NOV-2020 Trevor Y. H. Ho Baojun Wang annotation5 1 BBa_B0030 #c7b0e3 #c7b0e3 BBa_B0030 range 1 394 408 annotation10 1 pSEVA PS2 Rev #85dae9 #85dae9 pSEVA PS2 Rev range 1 1262 1278 annotation6 1 L3S2P11 #ff0000 #ff0000 L3S2P11 range 1 1117 1173 annotation7 1 Rep101 #993366 #993366 Rep101 range 1 2644 3594 annotation1 1 fd terminator #c6c9d1 #c6c9d1 fd terminator range 1 2339 2378 annotation0 1 AmpR #b7e6d7 #b7e6d7 AmpR range 1 1475 2335 annotation8 1 rrnB T1 terminator #c6c9d1 #c6c9d1 rrnB T1 terminator range 1 140 226 annotation12 1 lambda t0 terminator #c6c9d1 #c6c9d1 lambda t0 terminator range 1 1208 1302 annotation9 1 PSrpR #b7e6d7 #b7e6d7 PSrpR range 1 319 385 annotation4 1 AmpR promoter #c6c9d1 #c6c9d1 AmpR promoter range 1 1383 1474 annotation2 1 pSEVA PS1 Fwd #85dae9 #85dae9 pSEVA PS1 Fwd range 1 130 147 annotation11 1 2ndVal-mScarlet-I #ff9ccd #ff9ccd 2ndVal-mScarlet-I range 1 415 1113 annotation3 1 pSC101 ori #ffef86 #ffef86 pSC101 ori range 1 3642 3864 IBMc330_seq 1 ATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAAGCGGATAACAATTTCACACAGGAGGCCGCCTAGGCCGCGGCCGCGCGAATTCcttctagagGAAGACagtacgagaggattcgttaccaattgacagctagctcagtcctaggtATATACATACATGCTTGTTTGTTTGTAAACTACTAGAGATTAAAGAGGAGAAATACTAGatggtgagtaaaggagaagctgtgattaaagagttcatgcgcttcaaagttcacatggagggttctatgaacggtcacgagttcgagatcgaaggcgaaggcgagggccgtccgtatgaaggcacccagaccgccaaactgaaagtgactaaaggcggcccgctgcctttttcctgggacatcctgagcccgcaatttatgtacggttctagggcgttcatcaaacacccagcggatatcccggactattataagcagtcttttccggaaggtttcaagtgggaacgcgtaatgaattttgaagatggtggtgccgtgaccgtcactcaggacacctccctggaggatggcaccctgatctataaagttaaactgcgtggtactaattttccacctgatggcccggtgatgcagaaaaagacgatgggttgggaggcgtctaccgaacgcttgtatccggaagatggtgtgctgaaaggcgacattaaaatggccctgcgcctgaaagatggcggccgctatctggctgacttcaaaaccacgtacaaagccaagaaacctgtgcagatgcctggcgcgtacaatgtggaccgcaaactggacatcacctctcataatgaagattatacggtggtagagcaatatgagcgctccgagggtcgtcattctaccggtggcatggatgaactatacaaataataaCTCGGTACCAAATTCCAGAAAAGAGACGCTTTCGAGCGTCTTTTTTCGTTTTGGTCCgtacagctgcGTCTTCtactagtaCTGCAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTaaattagtagcccgcctaatgagcgggcttttttttaattcccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagcattcagcattttcgtgtggcgctgattccgttttttgcggcgttttgcctgccggtgtttgcgcatccggaaaccctggtgaaagtgaaagatgcggaagatcaactgggtgcgcgcgtgggctatattgaactggatctgaacagcggcaaaattctggaatcttttcgtccggaagaacgttttccgatgatgagcacctttaaagtgctgctgtgcggtgcggttctgagccgtgtggatgcgggccaggaacaactgggccgtcgtattcattatagccagaacgatctggtggaatatagcccggtgaccgaaaaacatctgaccgatggcatgaccgtgcgtgaactgtgcagcgcggcgattaccatgagcgataacaccgcggcgaacctgctgctgacgaccattggcggtccgaaagaactgaccgcgtttctgcataacatgggcgatcatgtgacccgtctggatcgttgggaaccggaactgaacgaagcgattccgaacgatgaacgtgataccaccatgccggcagcaatggcgaccaccctgcgtaaactgctgacgggtgagctgctgaccctggcaagccgccagcaactgattgattggatggaagcggataaagtggcgggtccgctgctgcgtagcgcgctgccggctggctggtttattgcggataaaagcggtgcgggcgaacgtggcagccgtggcattattgcggcgctgggcccggatggtaaaccgagccgtattgtggtgatttataccaccggcagccaggcgacgatggatgaacgtaaccgtcagattgcggaaattggcgcgagcctgattaaacattggtaaaccgatacaattaaaggctccttttggagcctttttttttggacgacccTTGTCCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTAGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAAT