LOCUS       IBMc331                 2597 bp    ds-DNA  circular     18-NOV-2020
DEFINITION  pSB1C3(BsmBI-,BsaI+)-BsaI-SrpR(23-192)-BsaI
ACCESSION   IBMc331
VERSION     IBMc331.1  
FEATURES             Location/Qualifiers
     rep_origin      complement(1698..2312)
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /label="rep (pMB1)"
     promoter        complement(661..758)
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="cat promoter"
     misc_feature    890..911
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /label="RFC10 Prefix (full)"
     misc_feature    1438..1458
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /label="RFC10 Suffix"
     primer_bind     772..791
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /label="VF2"
     terminator      complement(2483..2577)
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="lambda t0 terminator"
     CDS             919..1430
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /label="SrpR"
     CDS             complement(1..660)
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /label="cat (BsmBI-)"
     primer_bind     complement(1592..1611)
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /label="VR"
ORIGIN
        1 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
       61 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
      121 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
      181 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaAACga aaaacatatt
      241 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
      301 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
      361 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
      421 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
      481 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
      541 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
      601 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
      661 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
      721 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      781 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      841 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      901 cgcttctaga gGGTCTCAAA GTTTTTGTTG CACAGGGTGT TAGTGATGCA ACCCTGGATC
      961 AGATTGCACG TAAAGCCGGT GTTACCCGTG GTGCAGTTTA TTGGCATTTT AATGGTAAAC
     1021 TGGAAGTTCT GCAAGCAGTT CTGGCAAGCC GTCAGCATCC GCTGGAACTG GATTTTACAC
     1081 CGGATCTGGG TATTGAACGT AGCTGGGAAG CAGTTGTTGT TGCAATGCTG GATGCAGTTC
     1141 ATAGTCCGCA GAGCAAACAG TTTAGCGAAA TTCTGATTTA TCAGGGTCTG GATGAAAGCG
     1201 GTCTGATTCA TAATCGTATG GTTCAGGCAA GCGATCGTTT TCTGCAATAT ATTCATCAGG
     1261 TTCTGCGTCA TGCAGTTACC CAGGGTGAAC TGCCGATTAA TCTGGATCTG CAAACCAGCA
     1321 TTGGTGTTTT TAAAGGTCTG ATTACCGGTC TGCTGTATGA AGGTCTGCGT AGCAAAGATC
     1381 AGCAGGCACA GATTATCAAA GTTGCACTGG GTAGCTTTTG GGCACTGCTG TGAGACCtac
     1441 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     1501 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     1561 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     1741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2461 gtctcgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     2521 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     2581 gcgagctcga tatcaaa
//