https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
type: ComponentDefinition
persistent identity: IBMc334
display id: IBMc334
version: 1
description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter
molecule: ds-DNA
owned by: trevorho
mutable description: pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-FKBP-T-cat-PBAD(SapI-)-RiboJ-BCD1-FRB-NpuCcage-CFNS-mCherry*(193-236)-H6-Ter Expression plasmid for mCherry split at 192/193 inserted with Npu DnaE, caged intein fused with FKBP/FRB
mutable notes: Creator: Trevor Y. H. Ho Principal Investigator: Baojun Wang BioSafety Level: Level 1 Backbone: pSB3T5 Origin of replication: p15A Selection Markers: Tetracycline/ Chloramphenicol
date: 18-NOV-2020
creator: Trevor Y. H. Ho
creator: Baojun Wang
type: DnaRegion
type: SO:0000988
role: SO:0000637
sequence annotation: tetR promoter
sequence annotation: tetR
sequence annotation: VF2
sequence annotation: RFC10 Prefix (full)
sequence annotation: araC
sequence annotation: PBAD(SapI-)
sequence annotation: BBa_B0030
sequence annotation: mCherry*(1-192)
sequence annotation: NpuN(E52K,E54K,D85K,E89R,E91K)
sequence annotation: Caspase3 cut site
sequence annotation: (NpuC1-30(K2E,R6E,K11E))x2
sequence annotation: FKBP E coli optimized
sequence annotation: ECK120029600
sequence annotation: ECK120033737
sequence annotation: cat (BsmBI-)
sequence annotation: cat promoter
sequence annotation: L3S2P55
sequence annotation: RiboJ
sequence annotation: BCD1
sequence annotation: RBS for CDS 1
sequence annotation: RBS for CDS2
sequence annotation: FRB E coli optimized
sequence annotation: NpuN(51-102)
sequence annotation: NpuC
sequence annotation: mCherry*(193-236)
sequence annotation: H6
sequence annotation: ECK120033736
sequence annotation: RFC10 Suffix
sequence annotation: VR
sequence annotation: p15A origin of replication
sequence: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334_seq/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1
display id: annotation1
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: tetR promoter
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation1/range/1
role: SO:0000167

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 9
end: 97
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation7
display id: annotation7
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: tetR
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation7/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 98
end: 1291
orientation: inline

VF2
type: SequenceAnnotation
persistent identity: annotation22
display id: annotation22
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: VF2
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation22/range/1
role: SO:0005850

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 1371
end: 1390
orientation: inline

RFC10 Prefix (full)
type: SequenceAnnotation
persistent identity: annotation4
display id: annotation4
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: RFC10 Prefix (full)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation4/range/1
role: SO:0001411

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 1494
end: 1515
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation9
display id: annotation9
version: 1
ap einfo fwdcolor: #75c6a9
ap einfo revcolor: #75c6a9
label: araC
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation9/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 1516
end: 2394
orientation: reverseComplement

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation31
display id: annotation31
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation31/range/1
role: SO:0000167

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 2422
end: 2705
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation14
display id: annotation14
version: 1
ap einfo fwdcolor: #c7b0e3
ap einfo revcolor: #c7b0e3
label: BBa_B0030
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation14/range/1
role: SO:0000139

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 2714
end: 2728
orientation: inline

mCherry*(1-192)
type: SequenceAnnotation
persistent identity: annotation28
display id: annotation28
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(1-192)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation28/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 2735
end: 3309
orientation: inline

NpuN(E52K,E54K,D85K,E89R,E91K)
type: SequenceAnnotation
persistent identity: annotation11
display id: annotation11
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: NpuN(E52K,E54K,D85K,E89R,E91K)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation11/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 3323
end: 3628
orientation: inline

Caspase3 cut site
type: SequenceAnnotation
persistent identity: annotation8
display id: annotation8
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: Caspase3 cut site
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation8/range/1
role: SO:0000316

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation8/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 3644
end: 3655
orientation: inline

(NpuC1-30(K2E,R6E,K11E))x2
type: SequenceAnnotation
persistent identity: annotation24
display id: annotation24
version: 1
ap einfo fwdcolor: #d6b295
ap einfo revcolor: #d6b295
label: (NpuC1-30(K2E,R6E,K11E))x2
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation24/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 3680
end: 3862
orientation: inline

FKBP E coli optimized
type: SequenceAnnotation
persistent identity: annotation6
display id: annotation6
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: FKBP E coli optimized
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation6/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 3884
end: 4204
orientation: inline

ECK120029600
type: SequenceAnnotation
persistent identity: annotation3
display id: annotation3
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: ECK120029600
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation3/range/1
role: SO:0000141

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 4216
end: 4305
orientation: inline

ECK120033737
type: SequenceAnnotation
persistent identity: annotation0
display id: annotation0
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033737
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation0/range/1
role: SO:0000141

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation0/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 4314
end: 4370
orientation: reverseComplement

cat (BsmBI-)
type: SequenceAnnotation
persistent identity: annotation29
display id: annotation29
version: 1
ap einfo fwdcolor: #ff9ccd
ap einfo revcolor: #ff9ccd
label: cat (BsmBI-)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation29/range/1
role: SO:0000316

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation29/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 4399
end: 5058
orientation: reverseComplement

cat promoter
type: SequenceAnnotation
persistent identity: annotation26
display id: annotation26
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: cat promoter
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation26/range/1
role: SO:0000167

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation26/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5059
end: 5156
orientation: reverseComplement

L3S2P55
type: SequenceAnnotation
persistent identity: annotation23
display id: annotation23
version: 1
ap einfo fwdcolor: #ff0000
ap einfo revcolor: #ff0000
label: L3S2P55
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation23/range/1
role: SO:0000141

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation23/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5165
end: 5221
orientation: inline

PBAD(SapI-)
type: SequenceAnnotation
persistent identity: annotation20
display id: annotation20
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: PBAD(SapI-)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation20/range/1
role: SO:0000167

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation20/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5241
end: 5524
orientation: inline

RiboJ
type: SequenceAnnotation
persistent identity: annotation17
display id: annotation17
version: 1
ap einfo fwdcolor: #b1ff67
ap einfo revcolor: #b1ff67
label: RiboJ
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
feature type: insulator
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation17/range/1
role: SO:0000627

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation17/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5530
end: 5604
orientation: inline

BCD1
type: SequenceAnnotation
persistent identity: annotation10
display id: annotation10
version: 1
ap einfo fwdcolor: #008000
ap einfo revcolor: #008000
label: BCD1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation10/range/1
role: SO:0001411

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5605
end: 5689
orientation: inline

RBS for CDS 1
type: SequenceAnnotation
persistent identity: annotation15
display id: annotation15
version: 1
ap einfo fwdcolor: #ffef86
ap einfo revcolor: #ffef86
label: RBS for CDS 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation15/range/1
role: SO:0000139

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type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 5624
end: 5631
orientation: inline

RBS for CDS2
type: SequenceAnnotation
persistent identity: annotation12
display id: annotation12
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: RBS for CDS2
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owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation12/range/1
role: SO:0000139

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5675
end: 5683
orientation: inline

FRB E coli optimized
type: SequenceAnnotation
persistent identity: annotation18
display id: annotation18
version: 1
ap einfo fwdcolor: #b7e6d7
ap einfo revcolor: #b7e6d7
label: FRB E coli optimized
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation18/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5693
end: 5971
orientation: inline

NpuN(51-102)
type: SequenceAnnotation
persistent identity: annotation5
display id: annotation5
version: 1
ap einfo fwdcolor: #f58a5e
ap einfo revcolor: #f58a5e
label: NpuN(51-102)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation5/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 5987
end: 6142
orientation: inline

Caspase3 cut site
type: SequenceAnnotation
persistent identity: annotation13
display id: annotation13
version: 1
ap einfo fwdcolor: #85dae9
ap einfo revcolor: #85dae9
label: Caspase3 cut site
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation13/range/1
role: SO:0000316

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation13/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6158
end: 6169
orientation: inline

NpuC
type: SequenceAnnotation
persistent identity: annotation2
display id: annotation2
version: 1
ap einfo fwdcolor: #f8d3a9
ap einfo revcolor: #f8d3a9
label: NpuC
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation2/range/1
role: SO:0000316

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type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6185
end: 6298
orientation: inline

mCherry*(193-236)
type: SequenceAnnotation
persistent identity: annotation30
display id: annotation30
version: 1
ap einfo fwdcolor: #faac61
ap einfo revcolor: #faac61
label: mCherry*(193-236)
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation30/range/1
role: SO:0000316

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation30/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6302
end: 6433
orientation: inline

H6
type: SequenceAnnotation
persistent identity: annotation27
display id: annotation27
version: 1
ap einfo fwdcolor: #c6c9d1
ap einfo revcolor: #c6c9d1
label: H6
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation27/range/1
role: SO:0000316

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation27/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6434
end: 6451
orientation: inline

ECK120033736
type: SequenceAnnotation
persistent identity: annotation25
display id: annotation25
version: 1
ap einfo fwdcolor: #d59687
ap einfo revcolor: #d59687
label: ECK120033736
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation25/range/1
role: SO:0000141

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation25/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6463
end: 6515
orientation: inline

RFC10 Suffix
type: SequenceAnnotation
persistent identity: annotation21
display id: annotation21
version: 1
ap einfo fwdcolor: #9eafd2
ap einfo revcolor: #9eafd2
label: RFC10 Suffix
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation21/range/1
role: SO:0001411

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation21/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6516
end: 6536
orientation: inline

VR
type: SequenceAnnotation
persistent identity: annotation19
display id: annotation19
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: VR
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation19/range/1
role: SO:0005850

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation19/range/1
type: Range
persistent identity: range
display id: range
version: 1
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
start: 6631
end: 6650
orientation: reverseComplement

p15A origin of replication
type: SequenceAnnotation
persistent identity: annotation16
display id: annotation16
version: 1
ap einfo fwdcolor: #b4abac
ap einfo revcolor: #b4abac
label: p15A origin of replication
top level: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/1
owned by: trevorho
location: https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation16/range/1
role: SO:0000296

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334/annotation16/range/1
type: Range
persistent identity: range
display id: range
version: 1
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owned by: trevorho
start: 7097
end: 7921
orientation: inline

https://synbiohub.org/public/Intein_assisted_Bisection_Mapping/IBMc334_seq/1
type: Sequence
persistent identity: IBMc334 seq
display id: IBMc334_seq
version: 1
owned by: trevorho
elements: tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggt…
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