LOCUS IBMc335 8366 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-GAEY-e-NpuNcage-acVHH -T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-NpuCcage-CFNS-mCherry*(193-236) -H6-Ter ACCESSION IBMc335 VERSION IBMc335.1 FEATURES Location/Qualifiers CDS 6416..6547 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" terminator complement(4350..4406) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 6299..6412 /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="NpuC" terminator 4252..4341 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 6272..6283 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="Caspase3 cut site" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 3680..3862 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="(NpuC1-30(K2E,R6E,K11E))x2" CDS 3644..3655 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="Caspase3 cut site" CDS 6101..6256 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="NpuN(51-102)" misc_feature 5641..5725 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 5729..6085 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" RBS 5711..5719 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" CDS 2735..3309 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" CDS 3884..4240 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" RBS 5660..5667 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" rep_origin 7211..8035 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" misc_feature 5566..5640 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" primer_bind complement(6745..6764) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 5277..5560 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 6630..6650 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 5201..5257 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" terminator 6577..6629 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 3323..3628 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="NpuN(E52K,E54K,D85K,E89R,E91K)" promoter complement(5095..5192) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 6548..6565 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(4435..5094) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccGTT GGAGCAGAAT ATTGCCTGAG CTATGAAACC GAAATTCTGA CCGTTGAATA 3361 TGGTCTGCTG CCGATTGGTA AAATTGTGGA AAAACGTATT GAGTGCACCG TGTATAGCGT 3421 GGATAATAAC GGTAACATTT ATACCCAGCC GGTTGCACAG TGGCATGATC GTGGTAAACA 3481 GAAAGTTTTT GAGTACTGTC TGGAAGATGG TAGCCTGATT CGTGCAACCA AAGATCACAA 3541 ATTTATGACC GTGGATGGTC AGATGCTGCC CATTAAAGAA ATTTTTCGTC GTAAACTGGA 3601 TCTGATGCGT GTTGATAATC TGCCGAATGA AAATCTGTAT GGTGATGAAG TGGATGGCTA 3661 TTTTCAGGGT ATTGAAATTG CCACCGAAAA ATATCTGGGT GAGCAGAACG TTTATGATAT 3721 TGGTGTTGAA CGCGATCATA ACTTCGCACT GAAAAATGGT GGTTATTTCC AGGGCATCGA 3781 AATCGCAACA GAGAAATACC TGGGCGAACA GAATGTGTAT GACATCGGCG TGGAACGTGA 3841 TCACAATTTT GCCCTGAAGA ATGGCGGTAG CGGTAGCGCA AGCGAAGTTC AGCTGCAAGC 3901 AAGCGGTGGT GGTCTGGTTC AGGCAGGCGG TAGCCTGCGT CTGAGCTGTA CCGCAAGCGG 3961 TCGTACCGGC ACCATTTATA GCATGGCATG GTTTCGTCAG GCACCGGGTA AAGAACGTGA 4021 ATTTCTGGCA ACCGTTGGTT GGAGCAGCGG TATTACCTAT TATATGGATA GCGTGAAAGG 4081 TCGCTTTACC ATTAGCCGTG ATAATGCAAA AAATAGCGCC TACCTGCAAA TGAATAGCCT 4141 GAAACCGGAA GATACCGCAG TGTATTATTG TACAGCAACC CGTGCATATA GCGTGGGTTA 4201 TGATTATTGG GGTCAGGGCA CCCAGGTTAC CGTTAGCCAT taaTACTAGA Gttgagaaga 4261 gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac aagaccggcg 4321 gtcttaagtt ttttggctga aTACTAGAGc ctttggtcgA AAAAAAAAGC CCGCACTGTC 4381 AGGTGCGGGC TTTTTTctgt gtttccTACT AGAGCAAGCG AGCTCGATAT CAAATTACGC 4441 CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA 4501 GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG 4561 CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT 4621 TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAA ACGAAAAACA TATTCTCAAT 4681 AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT 4741 GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT 4801 TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC 4861 TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA 4921 GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG 4981 CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT 5041 ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC 5101 TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC 5161 ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCTACTAGAG CTCGGTACCA AAGACGAACA 5221 ATAAGACGCT GAAAAGCGTC TTTTTTCGTT TTGGTCCgct taacgatcgt tggctgagaa 5281 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gcActtctcg 5341 ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc 5401 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat 5461 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct 5521 acctgacgct ttttatcgca actctctact gtttctccat acccgagctg tcaccggatg 5581 tgctttccgg tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa 5641 GGGCCCAAGT TCACTTAAAA AGGAGATCAA CAATGAAAGC AATTTTCGTA CTGAAACATC 5701 TTAATCATGC ACAGGAGACT TTCTAatgGA AGTTCAGCTG CAAGCAAGCG GTGGTGGTCT 5761 GGTTCAGGCA GGCGGTAGCC TGCGTCTGAG CTGTACCGCA AGCGGTCGTA CCGGCACCAT 5821 TTATAGCATG GCATGGTTTC GTCAGGCACC GGGTAAAGAA CGTGAATTTC TGGCAACCGT 5881 TGGTTGGAGC AGCGGTATTA CCTATTATAT GGATAGCGTG AAAGGTCGCT TTACCATTAG 5941 CCGTGATAAT GCAAAAAATA GCGCCTACCT GCAAATGAAT AGCCTGAAAC CGGAAGATAC 6001 CGCAGTGTAT TATTGTACAG CAACCCGTGC ATATAGCGTG GGTTATGATT ATTGGGGTCA 6061 GGGCACCCAG GTTACCGTTA GCCATGGTAA TGGTAATGGT GGTGAACAAG AAGTGTTTGA 6121 GTATTGTCTG GAAGATGGTA GCCTGATTCG TGCAACCAAA GATCACAAAT TTATGACCGT 6181 TGATGGTCAG ATGCTGCCGA TTGATGAAAT TTTTGAACGT GAACTGGATC TGATGCGTGT 6241 TGATAATCTG CCGAATGAAA ATCTGTATGG TGATGAAGTG GATGGCTATT TTCAGGGTAT 6301 CAAAATTGCG ACCCGTAAAT ATCTGGGTAA ACAGAACGTT TATGATATTG GTGTGGAACG 6361 CGATCATAAC TTTGCACTGA AAAATGGTTT TATCGCCAGC AACTGCTTCA ATTCAcagct 6421 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 6481 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 6541 gtacaagcat catcaccatc accactaata ctagagaacg catgagAAAG CCCCCGGAAG 6601 ATCACCTTCC GGGGGCTTTt ttattgcgct actagtagcg gccgctgcag gagtcactaa 6661 gggttagtta gttagattag cagaaagtca aaagcctccg accggaggct tttgactaaa 6721 acttcccttg gggttatcat tggggctcac tcaaaggcgg taatcagata aaaaaaatcc 6781 ttagctttcg ctaaggatga tttctgctag agatggaata gactggatgg aggcggataa 6841 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 6901 tggagccggt gagcgtggat ctcgcggtat cattgcagca ctggggccag atggtaagcc 6961 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 7021 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 7081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 7141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 7201 gtcagacccc ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg 7261 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa 7321 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt 7381 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg 7441 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata 7501 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg 7561 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg 7621 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc aggggaaacg 7681 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt 7741 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc 7801 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc 7861 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat 7921 cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc 7981 acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct 8041 gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca ggcctgaatc gccccatcat 8101 ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg gaccagttgg 8161 tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga tgcgtgatct 8221 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc 8281 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt 8341 acataaacag taatacaagg ggtgtt //