##gff-version 3
##sequence-region IBMc335 9 8035
IBMc335	.	CDS	98	1291	.	+	0	ID=annotation25;Name=tetR
IBMc335	.	CDS	2735	3309	.	+	0	ID=annotation14;Name=mCherry*(1-192)
IBMc335	.	biological_region	5641	5725	.	+	0	ID=annotation10;Name=BCD1
IBMc335	.	terminator	4350	4406	.	-	0	ID=annotation1;Name=ECK120033737
IBMc335	.	CDS	6101	6256	.	+	0	ID=annotation9;Name=NpuN(51-102)
IBMc335	.	terminator	5201	5257	.	+	0	ID=annotation24;Name=L3S2P55
IBMc335	.	ribosome_entry_site	2714	2728	.	+	0	ID=annotation31;Name=BBa_B0030
IBMc335	.	promoter	9	97	.	+	0	ID=annotation11;Name=tetR promoter
IBMc335	.	primer_binding_site	1371	1390	.	+	0	ID=annotation6;Name=VF2
IBMc335	.	terminator	4252	4341	.	+	0	ID=annotation4;Name=ECK120029600
IBMc335	.	CDS	3680	3862	.	+	0	ID=annotation7;Name=(NpuC1-30(K2E,R6E,K11E))x2
IBMc335	.	CDS	4435	5094	.	-	0	ID=annotation30;Name=cat (BsmBI-)
IBMc335	.	promoter	5095	5192	.	-	0	ID=annotation28;Name=cat promoter
IBMc335	.	CDS	5729	6085	.	+	0	ID=annotation12;Name=acVHH
IBMc335	.	CDS	6299	6412	.	+	0	ID=annotation3;Name=NpuC
IBMc335	.	promoter	5277	5560	.	+	0	ID=annotation22;Name=PBAD(SapI-)
IBMc335	.	CDS	3644	3655	.	+	0	ID=annotation8;Name=Caspase3 cut site
IBMc335	.	promoter	2422	2705	.	+	0	ID=annotation17;Name=PBAD(SapI-)
IBMc335	.	primer_binding_site	6745	6764	.	-	0	ID=annotation20;Name=VR
IBMc335	.	insulator	5566	5640	.	+	0	ID=annotation19;Name=RiboJ
IBMc335	.	CDS	6548	6565	.	+	0	ID=annotation29;Name=H6
IBMc335	.	CDS	6272	6283	.	+	0	ID=annotation5;Name=Caspase3 cut site
IBMc335	.	CDS	3884	4240	.	+	0	ID=annotation15;Name=acVHH
IBMc335	.	terminator	6577	6629	.	+	0	ID=annotation26;Name=ECK120033736
IBMc335	.	ribosome_entry_site	5660	5667	.	+	0	ID=annotation16;Name=RBS for CDS 1
IBMc335	.	origin_of_replication	7211	8035	.	+	0	ID=annotation18;Name=p15A origin of replication
IBMc335	.	biological_region	6630	6650	.	+	0	ID=annotation23;Name=RFC10 Suffix
IBMc335	.	ribosome_entry_site	5711	5719	.	+	0	ID=annotation13;Name=RBS for CDS2
IBMc335	.	CDS	6416	6547	.	+	0	ID=annotation0;Name=mCherry*(193-236)
IBMc335	.	biological_region	1494	1515	.	+	0	ID=annotation21;Name=RFC10 Prefix (full)
IBMc335	.	CDS	1516	2394	.	-	0	ID=annotation2;Name=araC
IBMc335	.	CDS	3323	3628	.	+	0	ID=annotation27;Name=NpuN(E52K,E54K,D85K,E89R,E91K)
>IBMc335
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggc
tggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgat
aggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccct
aactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgc
tgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaat
cgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccg
gcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaa
atattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagc
ctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt
ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataa
aaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcag
gggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcaga
cattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaac
aaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaa
ctctctactgtttctccattactagagattaaagaggagaaatactagatggtgagcaagggcgaagaaGATaacatgg
ccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcga
gggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgg
gacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagc
tgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactc
ctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcag
aagAAAaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcaga
ggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccGTTGGAGCAGA
ATATTGCCTGAGCTATGAAACCGAAATTCTGACCGTTGAATATGGTCTGCTGCCGATTGGTAAAATTGTGGAAAAACGT
ATTGAGTGCACCGTGTATAGCGTGGATAATAACGGTAACATTTATACCCAGCCGGTTGCACAGTGGCATGATCGTGGTA
AACAGAAAGTTTTTGAGTACTGTCTGGAAGATGGTAGCCTGATTCGTGCAACCAAAGATCACAAATTTATGACCGTGGA
TGGTCAGATGCTGCCCATTAAAGAAATTTTTCGTCGTAAACTGGATCTGATGCGTGTTGATAATCTGCCGAATGAAAAT
CTGTATGGTGATGAAGTGGATGGCTATTTTCAGGGTATTGAAATTGCCACCGAAAAATATCTGGGTGAGCAGAACGTTT
ATGATATTGGTGTTGAACGCGATCATAACTTCGCACTGAAAAATGGTGGTTATTTCCAGGGCATCGAAATCGCAACAGA
GAAATACCTGGGCGAACAGAATGTGTATGACATCGGCGTGGAACGTGATCACAATTTTGCCCTGAAGAATGGCGGTAGC
GGTAGCGCAAGCGAAGTTCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCGTCTGAGCTGTA
CCGCAAGCGGTCGTACCGGCACCATTTATAGCATGGCATGGTTTCGTCAGGCACCGGGTAAAGAACGTGAATTTCTGGC
AACCGTTGGTTGGAGCAGCGGTATTACCTATTATATGGATAGCGTGAAAGGTCGCTTTACCATTAGCCGTGATAATGCA
AAAAATAGCGCCTACCTGCAAATGAATAGCCTGAAACCGGAAGATACCGCAGTGTATTATTGTACAGCAACCCGTGCAT
ATAGCGTGGGTTATGATTATTGGGGTCAGGGCACCCAGGTTACCGTTAGCCATtaaTACTAGAGttgagaagagaaaag
aaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACT
AGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCT
CGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCC
ATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTG
AAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAA
CGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATAT
GTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTG
TAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCA
GGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATC
CAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGAT
ATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAA
ATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCTACTAGAGCTCGGTACCAAAGA
CGAACAATAAGACGCTGAAAAGCGTCTTTTTTCGTTTTGGTCCgcttaacgatcgttggctgagaaaccaattgtccat
attgcatcagacattgccgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaag
cattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccac
attgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgct
ttttatcgcaactctctactgtttctccatacccgagctgtcaccggatgtgctttccggtctgatgagtccgtgagga
cgaaacagcctctacaaataattttgtttaaGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCG
TACTGAAACATCTTAATCATGCACAGGAGACTTTCTAatgGAAGTTCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAG
GCAGGCGGTAGCCTGCGTCTGAGCTGTACCGCAAGCGGTCGTACCGGCACCATTTATAGCATGGCATGGTTTCGTCAGG
CACCGGGTAAAGAACGTGAATTTCTGGCAACCGTTGGTTGGAGCAGCGGTATTACCTATTATATGGATAGCGTGAAAGG
TCGCTTTACCATTAGCCGTGATAATGCAAAAAATAGCGCCTACCTGCAAATGAATAGCCTGAAACCGGAAGATACCGCA
GTGTATTATTGTACAGCAACCCGTGCATATAGCGTGGGTTATGATTATTGGGGTCAGGGCACCCAGGTTACCGTTAGCC
ATGGTAATGGTAATGGTGGTGAACAAGAAGTGTTTGAGTATTGTCTGGAAGATGGTAGCCTGATTCGTGCAACCAAAGA
TCACAAATTTATGACCGTTGATGGTCAGATGCTGCCGATTGATGAAATTTTTGAACGTGAACTGGATCTGATGCGTGTT
GATAATCTGCCGAATGAAAATCTGTATGGTGATGAAGTGGATGGCTATTTTCAGGGTATCAAAATTGCGACCCGTAAAT
ATCTGGGTAAACAGAACGTTTATGATATTGGTGTGGAACGCGATCATAACTTTGCACTGAAAAATGGTTTTATCGCCAG
CAACTGCTTCAATTCAcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactac
accatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagcatcatcacc
atcaccactaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctactagt
agcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttga
ctaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaa
ggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttcc
ggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggccagat
ggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctg
agataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaact
tcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcg
ttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaa
acgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttgg
aggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatca
attaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcg
cagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggc
gtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgaggg
agccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtga
tgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggc
atcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtg
agcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaag
cacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgtgaag
aaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatgagagc
tttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatgcgtg
atctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattg
cacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt