LOCUS IBMc337 6557 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-deltaI-SMTR(WT)-S-m Cherry*(193-236)-H6-Ter ACCESSION IBMc337 VERSION IBMc337.1 FEATURES Location/Qualifiers CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" primer_bind complement(4936..4955) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 3314..3640 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="Mtu RecA 1-109" CDS 4607..4738 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" CDS 3647..4423 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="THRB" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 2735..3310 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" terminator 4768..4820 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" misc_feature 4821..4841 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" rep_origin 5402..6226 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS 4739..4756 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 4430..4603 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="MtuRecA 384-441" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 3301 gaagcccGTT GGATGCCTCG CAGAGGGCAC TCGGATCTTC GATCCGGTCA CCGGTACAAC 3361 GCATCGCATC GAGGATGTTG TCGATGGGCG CAAGCCTATT CATGTCGTGG CTGCTGCCAA 3421 GGACGGAACG CTGCATGCGC GGCCCGTGGT GTCCTGGTTC GACCAGGGAA CGCGGGATGT 3481 GATCGGGTTG CGGATCGCCG GTGGCGCCAT CCTGTGGGCG ACACCCGATC ACAAGGTGCT 3541 GACAGAGTAC GGCTGGCGTG CCGCCGGGGA ACTCCGCAAG GGAGACAGGG TGGCGCAACC 3601 GCGACGCTTC GATGGATTCG GTGACAGTGC GCCGATTCCG GCGCGCGAGC TGCAGAAGTC 3661 CATCGGGCAC AAGCCAGAGC CCACAGACGA GGAATGGGAG CTCATCAAAA CTGTCACCGA 3721 AGCCCATGTG GCGACCAACG CCCAAGGCAG CCACTGGAAG CAAAAACGGA AATTCCTGCC 3781 AGAAGACATT GGACAAGCAC CAATAGTCAA TGCCCCAGAA GGTGGAAAGG TTGACTTGGA 3841 AGCCTTCAGC CATTTTACAA AAATCATCAC ACCAGCAATT ACCAGAGTGG TGGATTTTGC 3901 CAAAAAGTTG CCTATGTTTT GTGAGCTGCC ATGTGAAGAC CAGATCATCC TCCTCAAAGG 3961 CTGCTGCATG GAGATCATGT CCCTTCGCGC TGCTGTGCGC TATGACCCAG AAAGTGAGAC 4021 TTTAACCTTG AATGGGGAAA TGGCAGTGAC ACGGGGCCAG CTGAAAAATG GGGGTCTTGG 4081 GGTGGTGTCA GACGCCATCT TTGACCTGGG CATGTCTCTG TCTTCTTTCA ACCTGGATGA 4141 CACTGAAGTA GCCCTCCTTC AGGCCGTCCT GCTGATGTCT TCAGATCGCC CGGGGCTTGC 4201 CTGTGTTGAG AGAATAGAAA AGTACCAAGA TAGTTTCCTG CTGGCCTTTG AACACTATAT 4261 CAATTACCGA AAACACCACG TGACACACTT TTGGCCAAAA CTCCTGATGA AGGTGACAGA 4321 TCTGCGGATG ATAGGAGCCT GCCATGCCAG CCGCTTCCTG CACATGAAGG TGGAATGCCC 4381 CACAGAACTC TTCCCCCCTT TGTTCTTGGA AGTGTTCGAG GATGCGCGCG TGCAGGCGCT 4441 CGCGGATGCC CTGGATGACA AATTCCTGCA CGACATGCTG GCGGAAGAAC TCCGCTATTC 4501 CGTGATCCGA GAAGTGCTGC CAACGCGGCG GGCACGAACG TTCGACCTCG AGGTCGAGGA 4561 ACTGCACACC CTCGTCGCCG AAGGGGTTGT CGTGCACAAC TGTTCAcagc tgcccggcgc 4621 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga 4681 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagca 4741 tcatcaccat caccactaat actagagaac gcatgagAAA GCCCCCGGAA GATCACCTTC 4801 CGGGGGCTTT tttattgcgc tactagtagc ggccgctgca ggagtcacta agggttagtt 4861 agttagatta gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt 4921 ggggttatca ttggggctca ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc 4981 gctaaggatg atttctgcta gagatggaat agactggatg gaggcggata aagttgcagg 5041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 5101 tgagcgtgga tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 5161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 5221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 5401 cttaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa 5461 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa 5521 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta 5581 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg 5641 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc 5701 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg 5761 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta 5821 aaccgaaagg caggaacagg agagcgcacg agggagccgc caggggaaac gcctggtatc 5881 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt 5941 caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag 6001 tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg 6061 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca 6121 catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg 6181 acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtctgc 6241 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga 6301 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga 6361 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca 6421 actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt 6481 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 6541 gtaatacaag gggtgtt //