LOCUS       IBMc339                 3161 bp    ds-DNA  circular     11-NOV-2020
DEFINITION  pSEVA171
ACCESSION   IBMc339
VERSION     IBMc339.1  
FEATURES             Location/Qualifiers
     terminator      1132..1171
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="fd terminator"
     primer_bind     complement(55..71)
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /label="pSEVA PS2 Rev"
     primer_bind     2914..2931
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /label="pSEVA PS1 Fwd"
     CDS             268..1128
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /label="AmpR"
     terminator      1..95
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="lambda t0 terminator"
     CDS             complement(1437..2387)
                     /ApEinfo_fwdcolor="#993366"
                     /ApEinfo_revcolor="#993366"
                     /label="Rep101"
     promoter        176..267
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="AmpR promoter"
     rep_origin      2435..2657
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /label="pSC101 ori"
     terminator      complement(2924..3010)
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /label="rrnB T1 terminator"
ORIGIN
        1 GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA TCTGGATTTG TTCAGAACGC
       61 TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAGG GGTCCCCAAT AATTACGATT
      121 TACGTATTTa aattagtagc ccgcctaatg agcgggcttt tttttaattc ccctatttgt
      181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      241 cttcaataat attgaaaaag gaagagtatg agcattcagc attttcgtgt ggcgctgatt
      301 ccgttttttg cggcgttttg cctgccggtg tttgcgcatc cggaaaccct ggtgaaagtg
      361 aaagatgcgg aagatcaact gggtgcgcgc gtgggctata ttgaactgga tctgaacagc
      421 ggcaaaattc tggaatcttt tcgtccggaa gaacgttttc cgatgatgag cacctttaaa
      481 gtgctgctgt gcggtgcggt tctgagccgt gtggatgcgg gccaggaaca actgggccgt
      541 cgtattcatt atagccagaa cgatctggtg gaatatagcc cggtgaccga aaaacatctg
      601 accgatggca tgaccgtgcg tgaactgtgc agcgcggcga ttaccatgag cgataacacc
      661 gcggcgaacc tgctgctgac gaccattggc ggtccgaaag aactgaccgc gtttctgcat
      721 aacatgggcg atcatgtgac ccgtctggat cgttgggaac cggaactgaa cgaagcgatt
      781 ccgaacgatg aacgtgatac caccatgccg gcagcaatgg cgaccaccct gcgtaaactg
      841 ctgacgggtg agctgctgac cctggcaagc cgccagcaac tgattgattg gatggaagcg
      901 gataaagtgg cgggtccgct gctgcgtagc gcgctgccgg ctggctggtt tattgcggat
      961 aaaagcggtg cgggcgaacg tggcagccgt ggcattattg cggcgctggg cccggatggt
     1021 aaaccgagcc gtattgtggt gatttatacc accggcagcc aggcgacgat ggatgaacgt
     1081 aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac attggtaaac cgatacaatt
     1141 aaaggctcct tttggagcct ttttttttgg acgacccTTG TCCTTTTCCG CTGCATAACC
     1201 CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA TCCTTTTTCG CACGATATAC
     1261 AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT ATCCAACGGC GTCAGCCGGG
     1321 CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC TTCTTCACTG TCCCTTATTC
     1381 GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC TGGCCGTAGG CCGGCCTCAG
     1441 ATCCTTCCGT ATTTAGCCAG TATGTTCTCT AGTGTGGTTC GTTGTTTTTG CGTGAGCCAT
     1501 GAGAACGAAC CATTGAGATC ATACTTACTT TGCATGTCAC TCAAAAATTT TGCCTCAAAA
     1561 CTGGTGAGCT GAATTTTTGC AGTTAAAGCA TCGTGTAGTG TTTTTCTTAG TCCGTTATGT
     1621 AGGTAGGAAT CTGATGTAAT GGTTGTTGGT ATTTTGTCAC CATTCATTTT TATCTGGTTG
     1681 TTCTCAAGTT CGGTTACGAG ATCCATTTGT CTATCTAGTT CAACTTGGAA AATCAACGTA
     1741 TCAGTCGGGC GGCCTCGCTT ATCAACCACC AATTTCATAT TGCTGTAAGT GTTTAAATCT
     1801 TTACTTATTG GTTTCAAAAC CCATTGGTTA AGCCTTTTAA ACTCATGGTA GTTATTTTCA
     1861 AGCATTAACA TGAACTTAAA TTCATCAAGG CTAATCTCTA TATTTGCCTT GTGAGTTTTC
     1921 TTTTGTGTTA GTTCTTTTAA TAACCACTCA TAAATCCTCA TAGAGTATTT GTTTTCAAAA
     1981 GACTTAACAT GTTCCAGATT ATATTTTATG AATTTTTTTA ACTGGAAAAG ATAAGGCAAT
     2041 ATCTCTTCAC TAAAAACTAA TTCTAATTTT TCGCTTGAGA ACTTGGCATA GTTTGTCCAC
     2101 TGGAAAATCT CAAAGCCTTT AACCAAAGGA TTCCTGATTT CCACAGTTCT CGTCATCAGC
     2161 TCTCTGGTTG CTTTAGCTAA TACACCATAA GCATTTTCCC TACTGATGTT CATCATCTGA
     2221 GCGTATTGGT TATAAGTGAA CGATACCGTC CGTTCTTTCC TTGTAGGGTT TTCAATCGTG
     2281 GGGTTGAGTA GTGCCACACA GCATAAAATT AGCTTGGTTT CATGCTCCGT TAAGTCATAG
     2341 CGACTAATCG CTAGTTCATT TGCTTTGAAA ACAACTAATT CAGACATACA TCTCAATTGG
     2401 TCTAGGTGAT TTTAATCACT ATACCAATTG AGATGGGCTA GTCAATGATA ATTACATGTC
     2461 CTTTTCCTTT GAGTTGTGGG TATCTGTAAA TTCTGCTAGA CCTTTGCTGG AAAACTTGTA
     2521 AATTCTGCTA GACCCTCTGT AAATTCCGCT AGACCTTTGT GTGTTTTTTT TGTTTATATT
     2581 CAAGTGGTTA TAATTTATAG AATAAAGAAA GAATAAAAAA AGATAAAAAG AATAGATCCC
     2641 AGCCCTGTGT ATAACTCACT ACTTTAGTCA GTTCCGCAGT ATTACAAAAG GATGTCGCAA
     2701 ACGCTGTTTG CTCCTCTACA AAACAGACCT TAAAACCCTA AAGGCTTAAG TAGCACCCTC
     2761 GCAAGCTCGG GCAAATCGCT GAATATTCCT TTTGTCTCCG ACCATCAGGC ACCTGAGTCG
     2821 CTGTCTTTTT CGTGACATTC AGTTCGCTGC GCTCACGGCT CTGGCAGTGA ATGGGGGTAA
     2881 ATGGCACTAC AGGCGCGGCG CGCCCAGCTG TCTAGGGCGG CGGATTTGTC CTACTCAGGA
     2941 GAGCGTTCAC CGACAAACAA CAGATAAAAC GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC
     3001 GTTTTATTTG TTTAATTAAA GCGGATAACA ATTTCACACA GGAGGCCGCC TAGGCCGCGG
     3061 CCGCGCGAAT TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG
     3121 CTTGCGGCCG CGTCGTGACT GGGAAAACCC TGGCGACTAG T
//