LOCUS IBMc372 6334 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-101)-G-M86(1-91)-HIG-ER-LBD-S -M86(92-154)-S-ECF20(102-193)-T ACCESSION IBMc372 VERSION IBMc372.1 FEATURES Location/Qualifiers CDS 4258..4533 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 102-193" CDS 3037..3309 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(1-91)" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" primer_bind complement(4713..4732) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 4066..4254 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(92-154)" RBS 2714..2724 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="BBa_B0033" CDS 2731..3033 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ECF20 1-101" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 4545..4597 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 3319..4062 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="ER-LBD" misc_feature 4598..4618 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" rep_origin 5179..6003 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcAct tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagtcacaca ggactactag atgaatgaaa ccgatcctga tctggaactg 2761 ctgaaacgta ttggtaataa tgatgcacag gccgttaaag aaatggttac ccgtaaactg 2821 cctcgtctgc tggcactggc aagtcgcctg ctgggtgatg cagatgaagc acgtgatatt 2881 gcacaagaaa gttttctgcg catttggaaa caggcagcaa gctggcgtag cgaacaggca 2941 cgttttgata cctggctgca tcgtgttgca ctgaatctgt gttatgatcg tctgcgtcgt 3001 cgtaaagaac atgtgccggt tgatagcgaa catGGAtgca tctcgggaga tagtttgatc 3061 agcttggcga gcacagggaa aagagttcct attaaggatt tgttaggcga aaaagatttt 3121 gaaatatggg caattaatga acagacgatg aagctggaat cagctaaagt tagtcgtgta 3181 ttttgtaccg gcaaaaagct agtctatact ctaaaaactc gactaggtag aactatcaag 3241 gcaacagcaa atcatagatt tttaactatt gatggttgga aaagattaga tgagctatct 3301 ttaaaagagc atatTGGAAA TAGCCTGGCA CTGAGCCTGA CCGCAGATCA GATGGTTAGC 3361 GCACTGCTGG ATGCAGAACC GCCTATTCTG TATAGCGAAT ATGATCCGAC ACGTCCGTTT 3421 AGCGAAGCAA GCATGATGGG TCTGCTGACC AATCTGGCAG ATCGTGAACT GGTTCATATG 3481 ATTAATTGGG CAAAACGTGT TCCGGGTTTT GTTGATCTGA CCCTGCATGA TCAGGTTCAT 3541 CTGCTGGAAT GTGCATGGCT GGAAATTCTG ATGATTGGTC TGGTTTGGCG TAGCATGGAA 3601 CATCCGGGTA AACTGCTGTT TGCACCGAAT CTGCTGCTGG ATCGTAATCA GGGTAAATGT 3661 GTTGAAGGTA TGGTGGAAAT CTTTGATATG CTGCTGGCAA CCAGCAGCCG TTTTCGTATG 3721 ATGAATCTGC AAGGTGAAGA ATTTGTTTGC CTGAAAAGCA TTATTCTGCT GAACAGCGGT 3781 GTTTATACCT TTCTGAGCAG CACCCTGAAA AGTCTGGAAG AAAAAGATCA TATCCATCGT 3841 GTGCTGGATA AAATCACCGA TACACTGATT CATCTGATGG CAAAAGCCGG TCTGACCTTA 3901 CAGCAGCAGC ATCAGCGTCT GGCACAACTG CTGCTGATTC TGAGCCATAT TCGTCATATG 3961 AGCAATAAAG GTATGGAACA CCTGTACAGC ATGAAATGCA AAAATGTTGT TCCGCTGTAT 4021 GACCTGCTGT TAGAAATGCT GGACGCACAT CGTCTGCATG CATCAcatat tgctctaccc 4081 cgtaaactag aaagctcctc tttacaattg gcaccagaaa tagaaaagtt gcctcagagt 4141 gatatttact gggaccccat cgtttctatt acggagactg gagtcgaaga ggtttttgat 4201 ttgactgtgc caggactacg taactttgtc gccaatgaca tcattgtaca taacTCAgcc 4261 tgtgaagcac tggatacccg tccggcaccg gatgaacagc tggaagcaag cgcacagagc 4321 cgtcgtatgg cacaggcact ggatcagctg ccggatcgtc agcgtgaagc aattgttctg 4381 cagtattatc aagaactgag caataccgaa gcagcagcac tgatgcaaat tagcgttgaa 4441 gccctggaaa gcctgctgag ccgtgcacgt cgtaatctgc gtagccatct ggccgaagca 4501 ccgggtgcag atctgagcgg tcgtcgcaaa ccgtaatact agagaacgca tgagAAAGCC 4561 CCCGGAAGAT CACCTTCCGG GGGCTTTttt attgcgctac tagtagcggc cgctgcagga 4621 gtcactaagg gttagttagt tagattagca gaaagtcaaa agcctccgac cggaggcttt 4681 tgactaaaac ttcccttggg gttatcattg gggctcactc aaaggcggta atcagataaa 4741 aaaaatcctt agctttcgct aaggatgatt tctgctagag atggaataga ctggatggag 4801 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 4861 gataaatctg gagccggtga gcgtggatct cgcggtatca ttgcagcact ggggccagat 4921 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 4981 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 5041 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 5101 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 5161 cactgagcgt cagacccctt aataagatga tcttcttgag atcgttttgg tctgcgcgta 5221 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga 5281 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc 5341 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc 5401 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt 5461 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga 5521 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca 5581 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag 5641 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca 5701 gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct 5761 ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa 5821 gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg 5881 gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca 5941 catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg 6001 ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac tcataccagg cctgaatcgc 6061 cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga 6121 ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg 6181 cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc 6241 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 6301 gtctgcttac ataaacagta atacaagggg tgtt //