##gff-version 3
##sequence-region IBMc378 1 7658
IBMc378	.	biological_region	5150	5234	.	+	0	ID=annotation26;Name=BCD1
IBMc378	.	origin_of_replication	6834	7658	.	+	0	ID=annotation24;Name=p15A origin of replication
IBMc378	.	terminator	4710	4766	.	+	0	ID=annotation5;Name=L3S2P55
IBMc378	.	promoter	4786	5069	.	+	0	ID=annotation3;Name=PBAD(SapI-)
IBMc378	.	primer_binding_site	1363	1382	.	+	0	ID=annotation2;Name=VF2
IBMc378	.	promoter	1	89	.	+	0	ID=annotation6;Name=tetR promoter
IBMc378	.	CDS	1508	2386	.	-	0	ID=annotation29;Name=araC
IBMc378	.	biological_region	6253	6273	.	+	0	ID=annotation4;Name=RFC10 Suffix
IBMc378	.	CDS	2727	3302	.	+	0	ID=annotation8;Name=mCherry*(1-192)
IBMc378	.	CDS	5625	6035	.	+	0	ID=annotation15;Name=M86(18-154)
IBMc378	.	CDS	5238	5594	.	+	0	ID=annotation21;Name=acVHH
IBMc378	.	terminator	3761	3850	.	+	0	ID=annotation14;Name=ECK120029600
IBMc378	.	ribosome_entry_site	5169	5176	.	+	0	ID=annotation27;Name=RBS for CDS 1
IBMc378	.	insulator	5075	5149	.	+	0	ID=annotation0;Name=RiboJ
IBMc378	.	promoter	2414	2697	.	+	0	ID=annotation12;Name=PBAD(SapI-)
IBMc378	.	CDS	3944	4603	.	-	0	ID=annotation9;Name=cat (BsmBI-)
IBMc378	.	ribosome_entry_site	5220	5228	.	+	0	ID=annotation23;Name=RBS for CDS2
IBMc378	.	terminator	6200	6252	.	+	0	ID=annotation7;Name=ECK120033736
IBMc378	.	promoter	4604	4701	.	-	0	ID=annotation28;Name=cat promoter
IBMc378	.	primer_binding_site	6368	6387	.	-	0	ID=annotation1;Name=VR
IBMc378	.	CDS	3306	3356	.	+	0	ID=annotation22;Name=M86(1-17)
IBMc378	.	biological_region	1486	1507	.	+	0	ID=annotation16;Name=RFC10 Prefix (full)
IBMc378	.	CDS	3363	3392	.	+	0	ID=annotation20;Name=2x(GGGGS) linker
IBMc378	.	ribosome_entry_site	2706	2720	.	+	0	ID=annotation25;Name=BBa_B0030
IBMc378	.	CDS	6171	6188	.	+	0	ID=annotation10;Name=H6
IBMc378	.	CDS	6039	6170	.	+	0	ID=annotation13;Name=mCherry*(193-236)
IBMc378	.	CDS	90	1283	.	+	0	ID=annotation19;Name=tetR
IBMc378	.	terminator	3859	3915	.	-	0	ID=annotation11;Name=ECK120033737
IBMc378	.	CDS	5595	5624	.	+	0	ID=annotation18;Name=2x(GGGGS) linker
IBMc378	.	CDS	3393	3749	.	+	0	ID=annotation17;Name=acVHH
>IBMc378
gagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtca
ggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttg
gttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttg
cgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcct
gctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccgga
cgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatc
gggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttggg
tgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatg
caggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggg
gcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggt
cattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgcc
ctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccg
acgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccgg
cggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcg
ctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacat
ggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccg
ggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaacc
gaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggccc
acccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgctt
ctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccg
gtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatcc
gggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctg
gcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgcc
aggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttcca
tgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaat
gatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgac
ggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggat
gacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatt
tttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcg
agataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcat
tttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccg
tcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcggg
accaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcg
tcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctac
tgtttctccattactagagattaaagaggagaaatactagatggtgagcaagggcgaagaaGATaacatggccatcatc
aaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagg
gccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcct
gtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttc
cccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgc
aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagAAAac
catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaag
ctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgGGAtgcatctcgggag
atagtttgatcagcttggcgagcacagggaaaagagttcctatTGGAGGCGGTGGTAGCGGTGGTGGCGGTAGCGAAGT
TCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCGTCTGAGCTGTACCGCAAGCGGTCGTACC
GGCACCATTTATAGCATGGCATGGTTTCGTCAGGCACCGGGTAAAGAACGTGAATTTCTGGCAACCGTTGGTTGGAGCA
GCGGTATTACCTATTATATGGATAGCGTGAAAGGTCGCTTTACCATTAGCCGTGATAATGCAAAAAATAGCGCCTACCT
GCAAATGAATAGCCTGAAACCGGAAGATACCGCAGTGTATTATTGTACAGCAACCCGTGCATATAGCGTGGGTTATGAT
TATTGGGGTCAGGGCACCCAGGTTACCGTTAGCCATtaaTACTAGAGttgagaagagaaaagaaaaccgccgatcctgt
ccaccgcattactgcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAA
AAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCC
CCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGAT
GAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAA
GTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCA
ATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGA
AATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACT
ATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGA
ATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGT
TATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATA
TCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGAT
CTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCTACTAGAGCTCGGTACCAAAGACGAACAATAAGACGCTG
AAAAGCGTCTTTTTTCGTTTTGGTCCgcttaacgatcgttggctgagaaaccaattgtccatattgcatcagacattgc
cgtcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcg
ggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacgg
cgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctct
actgtttctccatacccgagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaa
ataattttgtttaaGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAAT
CATGCACAGGAGACTTTCTAatgGAAGTTCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCG
TCTGAGCTGTACCGCAAGCGGTCGTACCGGCACCATTTATAGCATGGCATGGTTTCGTCAGGCACCGGGTAAAGAACGT
GAATTTCTGGCAACCGTTGGTTGGAGCAGCGGTATTACCTATTATATGGATAGCGTGAAAGGTCGCTTTACCATTAGCC
GTGATAATGCAAAAAATAGCGCCTACCTGCAAATGAATAGCCTGAAACCGGAAGATACCGCAGTGTATTATTGTACAGC
AACCCGTGCATATAGCGTGGGTTATGATTATTGGGGTCAGGGCACCCAGGTTACCGTTAGCCATGGTGGAGGTGGTTCA
GGTGGCGGAGGTTCAcctattaaggatttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatga
agctggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtag
aactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagag
catattgctctaccccgtaaactagaaagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtgata
tttactgggaccccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgtaa
ctttgtcgccaatgacatcattgtacataacTCAcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagcatcatcaccatcaccactaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTT
Ttttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcct
ccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaa
atccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccact
tctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcatt
gcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaac
gaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatact
ttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatc
ccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcg
taatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaa
ccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaag
actaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacga
tagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacc
cggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaa
caggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgattt
gagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccct
gttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgacc
gagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagcctttt
ttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgct
gaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgaggga
gccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcg
ttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaa
atctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatac
aaggggtgtttactagag