LOCUS IBMc379 7997 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-22)-LG-10aa-a cVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(23-154)-S-mCherry* (193-236)-H6-T ACCESSION IBMc379 VERSION IBMc379.1 FEATURES Location/Qualifiers promoter 4801..5084 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 6253..6273 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator 4725..4781 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" RBS 2706..2720 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 6200..6252 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(3959..4618) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" promoter 1..89 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 6171..6188 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" terminator complement(3874..3930) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 6039..6170 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(193-236)" rep_origin 6834..7658 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator 3776..3865 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" CDS 90..1283 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" CDS complement(1508..2386) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 5640..6035 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(23-154)" CDS 3408..3764 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 5610..5639 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="2x(GGGGS) linker" CDS 2727..3302 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*(1-192)" CDS 3378..3407 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="2x(GGGGS) linker" primer_bind 1363..1382 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 5253..5609 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 3306..3371 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86(1-22)" promoter 2414..2697 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" RBS 5235..5243 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="RBS for CDS2" misc_feature 5165..5249 /ApEinfo_fwdcolor="#008000" /ApEinfo_revcolor="#008000" /label="BCD1" RBS 5184..5191 /ApEinfo_fwdcolor="#ffef86" /ApEinfo_revcolor="#ffef86" /label="RBS for CDS 1" promoter complement(4619..4716) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" misc_feature 5090..5164 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="RiboJ" /featureType="insulator" primer_bind complement(6368..6387) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 1486..1507 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" ORIGIN 1 gagattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt 61 taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcattc 121 tcggcaccgt caccctggac gctgtaggca taggcttggt tatgccggta ctgccgggcc 181 tcttgcggga tatcgtccat tccgacagta ttgccagtca ctatggcgtg ctgcttgcgc 241 tctatgcgtt gatgcaattt ctttgcgcac ccgttctcgg agccctgtcc gaccgctttg 301 gccgccgtcc agtcctgctc gcttcgctcc ttggagccac tatcgactac gcgatcatgg 361 cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggcgggc atcacgggtg 421 ccacaggtgc ggttgctggt gcctatatcg ccgacatcac cgatggggaa gatcgggctc 481 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg 541 ggggactgtt gggtgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg 601 gcctcaacct cctcctgggc tgcttcctta tgcaggaatc gcataaggga gagcgccgtc 661 cgatgccctt gcgtgccttc aatccagtca gctccttccg gtgggcgcgg ggcatgacta 721 tcgtcgccgc acttatgact gttttcttta tcatgcaact cgtaggacag gttccggcag 781 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 841 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcacg ggccccgcca 901 ccaaacgttt cggcgagaag caggccatta tcgcgggcat ggcggccgac gcgctgggct 961 acgtcttgct ggcgttcgcg acgcgcggct ggatggcctt ccccattatg attcttctcg 1021 cttccggcgg catcggtatg cccgcgttgc aggccatgct gtcccgccaa gtagatgacg 1081 accatcaggg acagcttcaa gggtcgctcg cggctcttac cagcctcact tcgatcattg 1141 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat 1201 ggattgtagg tgccgccctt taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga 1261 gccgggccac ctcgacctaa taatactagc tccggcaaaa aaacgggcaa ggtgtcacca 1321 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct gacgtctaag 1381 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca 1441 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct 1501 tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 1561 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 1621 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 1681 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 1741 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 1801 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 1861 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 1921 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 1981 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 2041 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 2101 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 2161 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 2221 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 2281 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 2341 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 2401 cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt cactgcgtct 2461 tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc attctgtaac 2521 aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat cacggcagaa 2581 aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca 2641 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac 2701 tagagattaa agaggagaaa tactagatgg tgagcaaggg cgaagaaGAT aacatggcca 2761 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt 2821 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga 2881 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt 2941 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct 3001 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg 3061 tgacccagga ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca 3121 ccaacttccc ctccgacggc cccgtaatgc agaagAAAac catgggctgg gaggcctcct 3181 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc 3241 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg 3301 tgGGAtgcat ctcgggagat agtttgatca gcttggcgag cacagggaaa agagttccta 3361 ttaaggattt gttaggTGGA GGCGGTGGTA GCGGTGGTGG CGGTAGCGAA GTTCAGCTGC 3421 AAGCAAGCGG TGGTGGTCTG GTTCAGGCAG GCGGTAGCCT GCGTCTGAGC TGTACCGCAA 3481 GCGGTCGTAC CGGCACCATT TATAGCATGG CATGGTTTCG TCAGGCACCG GGTAAAGAAC 3541 GTGAATTTCT GGCAACCGTT GGTTGGAGCA GCGGTATTAC CTATTATATG GATAGCGTGA 3601 AAGGTCGCTT TACCATTAGC CGTGATAATG CAAAAAATAG CGCCTACCTG CAAATGAATA 3661 GCCTGAAACC GGAAGATACC GCAGTGTATT ATTGTACAGC AACCCGTGCA TATAGCGTGG 3721 GTTATGATTA TTGGGGTCAG GGCACCCAGG TTACCGTTAG CCATtaaTAC TAGAGttgag 3781 aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc 3841 ggcggtctta agttttttgg ctgaaTACTA GAGcctttgg tcgAAAAAAA AAGCCCGCAC 3901 TGTCAGGTGC GGGCTTTTTT ctgtgtttcc TACTAGAGCA AGCGAGCTCG ATATCAAATT 3961 ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT 4021 GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC 4081 CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA 4141 CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAAACGAAA AACATATTCT 4201 CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT 4261 ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT 4321 CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC 4381 CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA 4441 TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT 4501 CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT 4561 CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT 4621 TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA 4681 TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCTACT AGAGCTCGGT ACCAAAGACG 4741 AACAATAAGA CGCTGAAAAG CGTCTTTTTT CGTTTTGGTC Cgcttaacga tcgttggctg 4801 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggcActt 4861 ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa 4921 agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga 4981 ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga 5041 tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccga gctgtcaccg 5101 gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt 5161 ttaaGGGCCC AAGTTCACTT AAAAAGGAGA TCAACAATGA AAGCAATTTT CGTACTGAAA 5221 CATCTTAATC ATGCACAGGA GACTTTCTAa tgGAAGTTCA GCTGCAAGCA AGCGGTGGTG 5281 GTCTGGTTCA GGCAGGCGGT AGCCTGCGTC TGAGCTGTAC CGCAAGCGGT CGTACCGGCA 5341 CCATTTATAG CATGGCATGG TTTCGTCAGG CACCGGGTAA AGAACGTGAA TTTCTGGCAA 5401 CCGTTGGTTG GAGCAGCGGT ATTACCTATT ATATGGATAG CGTGAAAGGT CGCTTTACCA 5461 TTAGCCGTGA TAATGCAAAA AATAGCGCCT ACCTGCAAAT GAATAGCCTG AAACCGGAAG 5521 ATACCGCAGT GTATTATTGT ACAGCAACCC GTGCATATAG CGTGGGTTAT GATTATTGGG 5581 GTCAGGGCAC CCAGGTTACC GTTAGCCATG GTGGAGGTGG TTCAGGTGGC GGAGGTTCAt 5641 taggcgaaaa agattttgaa atatgggcaa ttaatgaaca gacgatgaag ctggaatcag 5701 ctaaagttag tcgtgtattt tgtaccggca aaaagctagt ctatactcta aaaactcgac 5761 taggtagaac tatcaaggca acagcaaatc atagattttt aactattgat ggttggaaaa 5821 gattagatga gctatcttta aaagagcata ttgctctacc ccgtaaacta gaaagctcct 5881 ctttacaatt ggcaccagaa atagaaaagt tgcctcagag tgatatttac tgggacccca 5941 tcgtttctat tacggagact ggagtcgaag aggtttttga tttgactgtg ccaggactac 6001 gtaactttgt cgccaatgac atcattgtac ataacTCAca gctgcccggc gcctacaacg 6061 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 6121 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag catcatcacc 6181 atcaccacta atactagaga acgcatgagA AAGCCCCCGG AAGATCACCT TCCGGGGGCT 6241 TTtttattgc gctactagta gcggccgctg caggagtcac taagggttag ttagttagat 6301 tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc ttggggttat 6361 cattggggct cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga 6421 tgatttctgc tagagatgga atagactgga tggaggcgga taaagttgca ggaccacttc 6481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 6541 gatctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 6601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 6661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 6721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 6781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa 6841 gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa 6901 ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta 6961 actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc 7021 atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt 7081 gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac 7141 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt 7201 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa 7261 ggcaggaaca ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt 7321 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg 7381 cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc 7441 tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa 7501 cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct 7561 gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc 7621 atcagtgcca acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa 7681 gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg 7741 gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc 7801 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca 7861 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca 7921 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca 7981 aggggtgttt actagag //