##gff-version 3
##sequence-region IBMc380 1 7658
IBMc380	.	biological_region	1486	1507	.	+	0	ID=annotation6;Name=RFC10 Prefix (full)
IBMc380	.	CDS	2727	3302	.	+	0	ID=annotation29;Name=mCherry*(1-192)
IBMc380	.	CDS	3306	3422	.	+	0	ID=annotation15;Name=M86(1-39)
IBMc380	.	CDS	5661	5690	.	+	0	ID=annotation8;Name=2x(GGGGS) linker
IBMc380	.	terminator	3827	3916	.	+	0	ID=annotation7;Name=ECK120029600
IBMc380	.	primer_binding_site	1363	1382	.	+	0	ID=annotation23;Name=VF2
IBMc380	.	terminator	3925	3981	.	-	0	ID=annotation4;Name=ECK120033737
IBMc380	.	CDS	5691	6035	.	+	0	ID=annotation5;Name=M86(40-154)
IBMc380	.	promoter	1	89	.	+	0	ID=annotation26;Name=tetR promoter
IBMc380	.	primer_binding_site	6368	6387	.	-	0	ID=annotation17;Name=VR
IBMc380	.	CDS	1508	2386	.	-	0	ID=annotation19;Name=araC
IBMc380	.	promoter	2414	2697	.	+	0	ID=annotation2;Name=PBAD(SapI-)
IBMc380	.	CDS	5304	5660	.	+	0	ID=annotation11;Name=acVHH
IBMc380	.	origin_of_replication	6834	7658	.	+	0	ID=annotation21;Name=p15A origin of replication
IBMc380	.	CDS	3429	3458	.	+	0	ID=annotation12;Name=2x(GGGGS) linker
IBMc380	.	terminator	4776	4832	.	+	0	ID=annotation25;Name=L3S2P55
IBMc380	.	ribosome_entry_site	5235	5242	.	+	0	ID=annotation16;Name=RBS for CDS 1
IBMc380	.	CDS	3459	3815	.	+	0	ID=annotation10;Name=acVHH
IBMc380	.	ribosome_entry_site	2706	2720	.	+	0	ID=annotation14;Name=BBa_B0030
IBMc380	.	CDS	90	1283	.	+	0	ID=annotation9;Name=tetR
IBMc380	.	insulator	5141	5215	.	+	0	ID=annotation20;Name=RiboJ
IBMc380	.	CDS	6171	6188	.	+	0	ID=annotation0;Name=H6
IBMc380	.	biological_region	5216	5300	.	+	0	ID=annotation18;Name=BCD1
IBMc380	.	terminator	6200	6252	.	+	0	ID=annotation27;Name=ECK120033736
IBMc380	.	CDS	4010	4669	.	-	0	ID=annotation1;Name=cat (BsmBI-)
IBMc380	.	promoter	4670	4767	.	-	0	ID=annotation28;Name=cat promoter
IBMc380	.	promoter	4852	5135	.	+	0	ID=annotation22;Name=PBAD(SapI-)
IBMc380	.	CDS	6039	6170	.	+	0	ID=annotation3;Name=mCherry*(193-236)
IBMc380	.	biological_region	6253	6273	.	+	0	ID=annotation24;Name=RFC10 Suffix
IBMc380	.	ribosome_entry_site	5286	5294	.	+	0	ID=annotation13;Name=RBS for CDS2
>IBMc380
gagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtca
ggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggcataggcttg
gttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgtgctgcttg
cgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgtccagtcct
gctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccgga
cgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatggggaagatc
gggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttggg
tgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgcttccttatg
caggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtgggcgcggg
gcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcgctctgggt
cattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgcc
ctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcatggcggccg
acgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctcgcttccgg
cggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttcaagggtcg
ctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacat
ggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgcatggagccg
ggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaacc
gaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggccc
acccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgctt
ctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccg
gtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatcc
gggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctg
gcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgcc
aggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttcca
tgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaat
gatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgac
ggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggat
gacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatt
tttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcg
agataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcat
tttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccg
tcactgcgtcttttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcggg
accaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcg
tcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctac
tgtttctccattactagagattaaagaggagaaatactagatggtgagcaagggcgaagaaGATaacatggccatcatc
aaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagg
gccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcct
gtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttc
cccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgc
aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagAAAac
catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaag
ctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgGGAtgcatctcgggag
atagtttgatcagcttggcgagcacagggaaaagagttcctattaaggatttgttaggcgaaaaagattttgaaatatg
ggcaattaatgaacagacgatgaagctggaTGGAGGCGGTGGTAGCGGTGGTGGCGGTAGCGAAGTTCAGCTGCAAGCA
AGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCGTCTGAGCTGTACCGCAAGCGGTCGTACCGGCACCATTTATA
GCATGGCATGGTTTCGTCAGGCACCGGGTAAAGAACGTGAATTTCTGGCAACCGTTGGTTGGAGCAGCGGTATTACCTA
TTATATGGATAGCGTGAAAGGTCGCTTTACCATTAGCCGTGATAATGCAAAAAATAGCGCCTACCTGCAAATGAATAGC
CTGAAACCGGAAGATACCGCAGTGTATTATTGTACAGCAACCCGTGCATATAGCGTGGGTTATGATTATTGGGGTCAGG
GCACCCAGGTTACCGTTAGCCATtaaTACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattact
gcaaggtagtggacaagaccggcggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCA
CTGTCAGGTGCGGGCTTTTTTctgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACT
CATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGC
CAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTG
GCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAG
GGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTA
TTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACC
AGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGAT
AAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTG
AGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTT
TTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTAT
GGTGAAAGTTGGAACCTCTTACGTGCCTACTAGAGCTCGGTACCAAAGACGAACAATAAGACGCTGAAAAGCGTCTTTT
TTCGTTTTGGTCCgcttaacgatcgttggctgagaaaccaattgtccatattgcatcagacattgccgtcactgcgtct
tttactggcActtctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccat
gacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgc
tatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
acccgagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgttta
aGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCACAGGAGA
CTTTCTAatgGAAGTTCAGCTGCAAGCAAGCGGTGGTGGTCTGGTTCAGGCAGGCGGTAGCCTGCGTCTGAGCTGTACC
GCAAGCGGTCGTACCGGCACCATTTATAGCATGGCATGGTTTCGTCAGGCACCGGGTAAAGAACGTGAATTTCTGGCAA
CCGTTGGTTGGAGCAGCGGTATTACCTATTATATGGATAGCGTGAAAGGTCGCTTTACCATTAGCCGTGATAATGCAAA
AAATAGCGCCTACCTGCAAATGAATAGCCTGAAACCGGAAGATACCGCAGTGTATTATTGTACAGCAACCCGTGCATAT
AGCGTGGGTTATGATTATTGGGGTCAGGGCACCCAGGTTACCGTTAGCCATGGTGGAGGTGGTTCAGGTGGCGGAGGTT
CActggaatcagctaaagttagtcgtgtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtag
aactatcaaggcaacagcaaatcatagatttttaactattgatggttggaaaagattagatgagctatctttaaaagag
catattgctctaccccgtaaactagaaagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtgata
tttactgggaccccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgtaa
ctttgtcgccaatgacatcattgtacataacTCAcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagcatcatcaccatcaccactaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTT
Ttttattgcgctactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcct
ccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaa
atccttagctttcgctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccact
tctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcatt
gcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaac
gaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatact
ttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatc
ccttaacgtgagttttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcg
taatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaa
ccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaag
actaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacga
tagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacc
cggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaa
caggagagcgcacgagggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgattt
gagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccct
gttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgacc
gagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagcctttt
ttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgct
gaggtctgcctcgtgaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgaggga
gccacggttgatgagagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcg
ttgtcgggaagatgcgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaa
atctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatac
aaggggtgtttactagag