LOCUS IBMc386 7738 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-171)-LNG-M86N-cat-Plux2-B3 2-M86C-S-mCherry*(172-236)-H6-T ACCESSION IBMc386 VERSION IBMc386.1 FEATURES Location/Qualifiers RBS 5538..5550 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 3257..3556 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 5476..5529 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 2735..3252 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator 5427..5467 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter complement(5392..5426) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" primer_bind complement(6117..6136) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" RBS complement(5369..5383) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" rep_origin 6583..7407 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(4607..5362) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" misc_feature 6002..6022 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator complement(4517..4573) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" terminator 5949..6001 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(4411..4508) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 5920..5937 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(3751..4410) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 5725..5919 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator complement(3666..3722) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5560..5721 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3568..3657 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcact tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaTGGAtgca tctcgggaga tagtttgatc agcttggcga gcacagggaa 3301 aagagttcct attaaggatt tgttaggcga aaaagatttt gaaatatggg caattaatga 3361 acagacgatg aagctggaat cagctaaagt tagtcgtgta ttttgtaccg gcaaaaagct 3421 agtctatact ctaaaaactc gactaggtag aactatcaag gcaacagcaa atcatagatt 3481 tttaactatt gatggttgga aaagattaga tgagctatct ttaaaagagc atattgctct 3541 accccgtaaa ctagaaTAAT ACTAGAGttg agaagagaaa agaaaaccgc cgatcctgtc 3601 caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaTAC 3661 TAGAGccttt ggtcgAAAAA AAAAGCCCGC ACTGTCAGGT GCGGGCTTTT TTctgtgttt 3721 ccTACTAGAG CAAGCGAGCT CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA 3781 CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC 3841 CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA 3901 AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC 3961 CCAGGGATTG GCTGAAACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG 4021 GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC 4081 GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA 4141 AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG 4201 ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT 4261 TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA 4321 TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC 4381 GGTGGTATAT CCAGTGATTT TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA 4441 TAACTCAAAA AATACGCCCG GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT 4501 TACGTGCCTA CTAGAGGGAC CAAAACGAAA AAAGACGCTT TTCAGCGTCT TATTGTTCGT 4561 CTTTGGTACC GAGCTCTAGT AGTGATCTAC ACTAGCACTA TCAGTGTTAT TAATTTTTAA 4621 AGTATGGGCA ATCAATTGCT CCTGTTAAAA TTGCTTTAGA AATACTTTGG CAGCGGTTTG 4681 TTGTATTGAG TTTCATTTGC GCATTGGTTA AATGGAAAGT GACAGTACGC TCACTGCAAC 4741 CTAATATTTT TGAAATATCC CAAGAGCTTT TTCCTTCGCA TGCCCACGCT AAACATTCTT 4801 TTTCTCTTTT GGTTAAATCG TTGTTTGATT TATTATTTGC TATATTTATT TTTCGATAAT 4861 TATCAACTAG AGAAGGAACA ATTAATGGTA TGTTCATACA CGCATGTAAA AATAAACTAT 4921 CTATATAGTT GTCTTTTTCT GAATGTGCAA AACTAAGCAT TCCGAAGCCA TTGTTAGCCG 4981 TATGAATAGG GAAACTAAAC CCAGTGATAA GACCTGATGT TTTCGCTTCT TTAATTACAT 5041 TTGGAGATTT TTTATTTACA GCATTGTTTT CAAATATATT CCAATTAATT GGTGAATGAT 5101 TGGAGTTAGA ATAATCTACT ATAGGATCAT ATTTTATTAA ATTAGCGTCA TCATAATATT 5161 GCCTCCATTT TTTAGGGTAA TTATCTAGGA TTGAAATATC AGATTTAACC ATAGAATGAG 5221 GATAAATGAT CGCGAGTAAA TAATATTCAC AATGTACCAT TTTAGTCATA TCAGATAAGC 5281 ATTGATTAAT ATCATTATTG CTTCTACAAG CTTTAATTTT ATTAATTATT CTGTATGTGT 5341 CGTCGGCATT TATGTTTTTC ATCTAGTATT TCTCCTCTTT AATCTCTAGT AGCTAGCACA 5401 ATCCCTAGGA CTGAGCTAGC TGTCAATCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC 5461 GTTTATATAC TAGACACCTG TAGGATCGTA CAGGTTTACG CAAGAAAATG GTTTGTTACT 5521 TTCGAATAAt actagagtca cacaggaaag tactagATGa gctcctcttt acaattggca 5581 ccagaaatag aaaagttgcc tcagagtgat atttactggg accccatcgt ttctattacg 5641 gagactggag tcgaagaggt ttttgatttg actgtgccag gactacgtaa ctttgtcgcc 5701 aatgacatca ttgtacataa cTCActgaag gacggcggcc actacgacgc tgaggtcaag 5761 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 5821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 5881 ggccgccact ccaccggcgg catggacgag ctgtacaagc atcatcacca tcaccactaa 5941 tactagagaa cgcatgagAA AGCCCCCGGA AGATCACCTT CCGGGGGCTT Ttttattgcg 6001 ctactagtag cggccgctgc aggagtcact aagggttagt tagttagatt agcagaaagt 6061 caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc 6121 actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct 6181 agagatggaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt 6301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 6601 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 6661 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 6721 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 6781 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 6901 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 6961 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 7021 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7081 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 7141 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 7201 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 7261 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 7321 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 7381 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc 7441 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt 7501 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga 7561 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt 7621 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 7681 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt //