##gff-version 3
##sequence-region IBMc386 9 7407
IBMc386	.	terminator	5427	5467	.	+	0	ID=annotation5;Name=BBa_B0012
IBMc386	.	terminator	3568	3657	.	+	0	ID=annotation26;Name=ECK120029600
IBMc386	.	CDS	2735	3252	.	+	0	ID=annotation4;Name=mCherry*
IBMc386	.	promoter	5476	5529	.	+	0	ID=annotation2;Name=Plux2
IBMc386	.	promoter	2422	2705	.	+	0	ID=annotation3;Name=PBAD(SapI-)
IBMc386	.	promoter	5392	5426	.	-	0	ID=annotation8;Name=BBa_J23117
IBMc386	.	CDS	3751	4410	.	-	0	ID=annotation21;Name=cat (BsmBI-)
IBMc386	.	primer_binding_site	1371	1390	.	+	0	ID=annotation22;Name=VF2
IBMc386	.	CDS	5920	5937	.	+	0	ID=annotation20;Name=H6
IBMc386	.	CDS	5560	5721	.	+	0	ID=annotation25;Name=M86C
IBMc386	.	primer_binding_site	6117	6136	.	-	0	ID=annotation9;Name=VR
IBMc386	.	promoter	4411	4508	.	-	0	ID=annotation18;Name=cat promoter
IBMc386	.	biological_region	1494	1515	.	+	0	ID=annotation6;Name=RFC10 Prefix (full)
IBMc386	.	CDS	98	1291	.	+	0	ID=annotation10;Name=tetR
IBMc386	.	CDS	3257	3556	.	+	0	ID=annotation1;Name=M86N
IBMc386	.	ribosome_entry_site	2714	2728	.	+	0	ID=annotation7;Name=BBa_B0030
IBMc386	.	CDS	4607	5362	.	-	0	ID=annotation14;Name=luxR
IBMc386	.	origin_of_replication	6583	7407	.	+	0	ID=annotation13;Name=p15A origin of replication
IBMc386	.	terminator	3666	3722	.	-	0	ID=annotation24;Name=ECK120033737
IBMc386	.	ribosome_entry_site	5538	5550	.	+	0	ID=annotation0;Name=BBa_B0032
IBMc386	.	promoter	9	97	.	+	0	ID=annotation11;Name=tetR promoter
IBMc386	.	CDS	1516	2394	.	-	0	ID=annotation19;Name=araC
IBMc386	.	biological_region	6002	6022	.	+	0	ID=annotation15;Name=RFC10 Suffix
IBMc386	.	ribosome_entry_site	5369	5383	.	-	0	ID=annotation12;Name=BBa_B0030
IBMc386	.	terminator	4517	4573	.	-	0	ID=annotation16;Name=L3S2P55
IBMc386	.	CDS	5725	5919	.	+	0	ID=annotation23;Name=mCherry*
IBMc386	.	terminator	5949	6001	.	+	0	ID=annotation17;Name=ECK120033736
>IBMc386
tactagaggagattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaa
cgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcattctcggcaccgtcaccctggacgctgtaggca
taggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagtattgccagtcactatggcgt
gctgcttgcgctctatgcgttgatgcaatttctttgcgcacccgttctcggagccctgtccgaccgctttggccgccgt
ccagtcctgctcgcttcgctccttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctct
acgccggacgcatcgtggcgggcatcacgggtgccacaggtgcggttgctggtgcctatatcgccgacatcaccgatgg
ggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggga
ctgttgggtgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctcctcctgggctgct
tccttatgcaggaatcgcataagggagagcgccgtccgatgcccttgcgtgccttcaatccagtcagctccttccggtg
ggcgcggggcatgactatcgtcgccgcacttatgactgttttctttatcatgcaactcgtaggacaggttccggcagcg
ctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatct
tgcacgccctcgctcaagccttcgtcacgggccccgccaccaaacgtttcggcgagaagcaggccattatcgcgggcat
ggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgcggctggatggccttccccattatgattcttctc
gcttccggcggcatcggtatgcccgcgttgcaggccatgctgtcccgccaagtagatgacgaccatcagggacagcttc
aagggtcgctcgcggctcttaccagcctcacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggc
gagcacatggaacgggttggcatggattgtaggtgccgccctttaccttgtctgcctccccgcgttgcgtcgcggtgca
tggagccgggccacctcgacctaataatactagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttct
ttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaag
aaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgc
ggccgcttctagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggc
tggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgat
aggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccct
aactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgc
tgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaat
cgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccg
gcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaa
atattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagc
ctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgt
ccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataa
aaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcag
gggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcaga
cattgccgtcactgcgtcttttactggcacttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaac
aaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattattt
gcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaa
ctctctactgtttctccattactagagattaaagaggagaaatactagatggtgagcaagggcgaagaaGATaacatgg
ccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcga
gggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgg
gacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagc
tgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactc
ctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcag
aagAAAaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcaga
ggctgaagctgaaTGGAtgcatctcgggagatagtttgatcagcttggcgagcacagggaaaagagttcctattaagga
tttgttaggcgaaaaagattttgaaatatgggcaattaatgaacagacgatgaagctggaatcagctaaagttagtcgt
gtattttgtaccggcaaaaagctagtctatactctaaaaactcgactaggtagaactatcaaggcaacagcaaatcata
gatttttaactattgatggttggaaaagattagatgagctatctttaaaagagcatattgctctaccccgtaaactaga
aTAATACTAGAGttgagaagagaaaagaaaaccgccgatcctgtccaccgcattactgcaaggtagtggacaagaccgg
cggtcttaagttttttggctgaaTACTAGAGcctttggtcgAAAAAAAAAGCCCGCACTGTCAGGTGCGGGCTTTTTTc
tgtgtttccTACTAGAGCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTC
ATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCG
CCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG
TGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACC
GTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAAC
GTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCA
TACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTT
TACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCA
AAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAG
CTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTAC
GTGCCTACTAGAGGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTTATTGTTCGTCTTTGGTACCGAGCTCTAGTAG
TGATCTACACTAGCACTATCAGTGTTATTAATTTTTAAAGTATGGGCAATCAATTGCTCCTGTTAAAATTGCTTTAGAA
ATACTTTGGCAGCGGTTTGTTGTATTGAGTTTCATTTGCGCATTGGTTAAATGGAAAGTGACAGTACGCTCACTGCAAC
CTAATATTTTTGAAATATCCCAAGAGCTTTTTCCTTCGCATGCCCACGCTAAACATTCTTTTTCTCTTTTGGTTAAATC
GTTGTTTGATTTATTATTTGCTATATTTATTTTTCGATAATTATCAACTAGAGAAGGAACAATTAATGGTATGTTCATA
CACGCATGTAAAAATAAACTATCTATATAGTTGTCTTTTTCTGAATGTGCAAAACTAAGCATTCCGAAGCCATTGTTAG
CCGTATGAATAGGGAAACTAAACCCAGTGATAAGACCTGATGTTTTCGCTTCTTTAATTACATTTGGAGATTTTTTATT
TACAGCATTGTTTTCAAATATATTCCAATTAATTGGTGAATGATTGGAGTTAGAATAATCTACTATAGGATCATATTTT
ATTAAATTAGCGTCATCATAATATTGCCTCCATTTTTTAGGGTAATTATCTAGGATTGAAATATCAGATTTAACCATAG
AATGAGGATAAATGATCGCGAGTAAATAATATTCACAATGTACCATTTTAGTCATATCAGATAAGCATTGATTAATATC
ATTATTGCTTCTACAAGCTTTAATTTTATTAATTATTCTGTATGTGTCGTCGGCATTTATGTTTTTCATCTAGTATTTC
TCCTCTTTAATCTCTAGTAGCTAGCACAATCCCTAGGACTGAGCTAGCTGTCAATCACACTGGCTCACCTTCGGGTGGG
CCTTTCTGCGTTTATATACTAGACACCTGTAGGATCGTACAGGTTTACGCAAGAAAATGGTTTGTTACTTTCGAATAAt
actagagtcacacaggaaagtactagATGagctcctctttacaattggcaccagaaatagaaaagttgcctcagagtga
tatttactgggaccccatcgtttctattacggagactggagtcgaagaggtttttgatttgactgtgccaggactacgt
aactttgtcgccaatgacatcattgtacataacTCActgaaggacggcggccactacgacgctgaggtcaagaccacct
acaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgagga
ctacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagcatcat
caccatcaccactaatactagagaacgcatgagAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTtttattgcgctac
tagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggctt
ttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcg
ctaaggatgatttctgctagagatggaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggccc
ttccggctggctggtttattgctgataaatctggagccggtgagcgtggatctcgcggtatcattgcagcactggggcc
agatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatc
gctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaa
aacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagtt
ttcgttccactgagcgtcagaccccttaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctct
gaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggc
ttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaa
atcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataa
ggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtc
aggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacg
agggagccgccaggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttc
gtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcc
tggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtc
agtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacat
gaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgaggtctgcctcgt
gaagaaggtgttgctgactcataccaggcctgaatcgccccatcatccagccagaaagtgagggagccacggttgatga
gagctttgttgtaggtggaccagttggtgattttgaacttttgctttgccacggaacggtctgcgttgtcgggaagatg
cgtgatctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttac
attgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgtt