LOCUS IBMc387 7738 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-173)-DGG-M86N-cat-Plux2-B3 2-M86C-S-mCherry*(174-236)-H6-T ACCESSION IBMc387 VERSION IBMc387.1 FEATURES Location/Qualifiers rep_origin 6583..7407 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(4613..5368) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" misc_feature 6002..6022 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator complement(4523..4579) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" terminator 5949..6001 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" promoter complement(4417..4514) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 5920..5937 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS complement(3757..4416) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 5731..5919 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator complement(3672..3728) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" CDS 5566..5727 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3574..3663 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" RBS 5544..5556 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 3263..3562 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" promoter 5482..5535 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" CDS 2735..3258 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator 5433..5473 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter complement(5398..5432) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" primer_bind complement(6117..6136) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS complement(5375..5389) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcact tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggTG GAtgcatctc gggagatagt ttgatcagct tggcgagcac 3301 agggaaaaga gttcctatta aggatttgtt aggcgaaaaa gattttgaaa tatgggcaat 3361 taatgaacag acgatgaagc tggaatcagc taaagttagt cgtgtatttt gtaccggcaa 3421 aaagctagtc tatactctaa aaactcgact aggtagaact atcaaggcaa cagcaaatca 3481 tagattttta actattgatg gttggaaaag attagatgag ctatctttaa aagagcatat 3541 tgctctaccc cgtaaactag aaTAATACTA GAGttgagaa gagaaaagaa aaccgccgat 3601 cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct 3661 gaaTACTAGA Gcctttggtc gAAAAAAAAA GCCCGCACTG TCAGGTGCGG GCTTTTTTct 3721 gtgtttccTA CTAGAGCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC 3781 GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG 3841 ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT 3901 GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA 3961 ACTCACCCAG GGATTGGCTG AAACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA 4021 GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA 4081 ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT 4141 GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA 4201 TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG 4261 CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA 4321 GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT 4381 ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA 4441 TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA 4501 ACCTCTTACG TGCCTACTAG AGGGACCAAA ACGAAAAAAG ACGCTTTTCA GCGTCTTATT 4561 GTTCGTCTTT GGTACCGAGC TCTAGTAGTG ATCTACACTA GCACTATCAG TGTTATTAAT 4621 TTTTAAAGTA TGGGCAATCA ATTGCTCCTG TTAAAATTGC TTTAGAAATA CTTTGGCAGC 4681 GGTTTGTTGT ATTGAGTTTC ATTTGCGCAT TGGTTAAATG GAAAGTGACA GTACGCTCAC 4741 TGCAACCTAA TATTTTTGAA ATATCCCAAG AGCTTTTTCC TTCGCATGCC CACGCTAAAC 4801 ATTCTTTTTC TCTTTTGGTT AAATCGTTGT TTGATTTATT ATTTGCTATA TTTATTTTTC 4861 GATAATTATC AACTAGAGAA GGAACAATTA ATGGTATGTT CATACACGCA TGTAAAAATA 4921 AACTATCTAT ATAGTTGTCT TTTTCTGAAT GTGCAAAACT AAGCATTCCG AAGCCATTGT 4981 TAGCCGTATG AATAGGGAAA CTAAACCCAG TGATAAGACC TGATGTTTTC GCTTCTTTAA 5041 TTACATTTGG AGATTTTTTA TTTACAGCAT TGTTTTCAAA TATATTCCAA TTAATTGGTG 5101 AATGATTGGA GTTAGAATAA TCTACTATAG GATCATATTT TATTAAATTA GCGTCATCAT 5161 AATATTGCCT CCATTTTTTA GGGTAATTAT CTAGGATTGA AATATCAGAT TTAACCATAG 5221 AATGAGGATA AATGATCGCG AGTAAATAAT ATTCACAATG TACCATTTTA GTCATATCAG 5281 ATAAGCATTG ATTAATATCA TTATTGCTTC TACAAGCTTT AATTTTATTA ATTATTCTGT 5341 ATGTGTCGTC GGCATTTATG TTTTTCATCT AGTATTTCTC CTCTTTAATC TCTAGTAGCT 5401 AGCACAATCC CTAGGACTGA GCTAGCTGTC AATCACACTG GCTCACCTTC GGGTGGGCCT 5461 TTCTGCGTTT ATATACTAGA CACCTGTAGG ATCGTACAGG TTTACGCAAG AAAATGGTTT 5521 GTTACTTTCG AATAAtacta gagtcacaca ggaaagtact agATGagctc ctctttacaa 5581 ttggcaccag aaatagaaaa gttgcctcag agtgatattt actgggaccc catcgtttct 5641 attacggaga ctggagtcga agaggttttt gatttgactg tgccaggact acgtaacttt 5701 gtcgccaatg acatcattgt acataacTCA gacggcggcc actacgacgc tgaggtcaag 5761 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 5821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 5881 ggccgccact ccaccggcgg catggacgag ctgtacaagc atcatcacca tcaccactaa 5941 tactagagaa cgcatgagAA AGCCCCCGGA AGATCACCTT CCGGGGGCTT Ttttattgcg 6001 ctactagtag cggccgctgc aggagtcact aagggttagt tagttagatt agcagaaagt 6061 caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc 6121 actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct 6181 agagatggaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt 6301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 6601 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 6661 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 6721 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 6781 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 6901 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 6961 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 7021 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7081 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 7141 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 7201 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 7261 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 7321 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 7381 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc 7441 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt 7501 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga 7561 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt 7621 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 7681 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt //