LOCUS IBMc389 7738 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-176)-HYG-M86N-cat-Plux2-B3 2-M86C-S-mCherry*(177-236)-H6-T ACCESSION IBMc389 VERSION IBMc389.1 FEATURES Location/Qualifiers terminator 5442..5482 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0012" RBS 2714..2728 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter complement(5407..5441) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="BBa_J23117" misc_feature 1494..1515 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind complement(6117..6136) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 98..1291 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="tetR" RBS complement(5384..5398) /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" promoter 9..97 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="tetR promoter" rep_origin 6583..7407 /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" CDS complement(4622..5377) /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="luxR" misc_feature 6002..6022 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator complement(4532..4588) /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="L3S2P55" terminator 5949..6001 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" promoter complement(4426..4523) /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" CDS 5920..5937 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="H6" CDS complement(1516..2394) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS complement(3766..4425) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat (BsmBI-)" CDS 5740..5919 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" terminator complement(3681..3737) /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033737" primer_bind 1371..1390 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS 5575..5736 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86C" terminator 3583..3672 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" RBS 5553..5565 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="BBa_B0032" CDS 3272..3571 /ApEinfo_fwdcolor="#84b0dc" /ApEinfo_revcolor="#84b0dc" /label="M86N" promoter 5491..5544 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="Plux2" CDS 2735..3267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="mCherry*" promoter 2422..2705 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" ORIGIN 1 tactagagga gattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt 61 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat 121 cgtcattctc ggcaccgtca ccctggacgc tgtaggcata ggcttggtta tgccggtact 181 gccgggcctc ttgcgggata tcgtccattc cgacagtatt gccagtcact atggcgtgct 241 gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc gttctcggag ccctgtccga 301 ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt ggagccacta tcgactacgc 361 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggcgggcat 421 cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc gacatcaccg atggggaaga 481 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc 541 cgtggccggg ggactgttgg gtgccatctc cttgcatgca ccattccttg cggcggcggt 601 gctcaacggc ctcaacctcc tcctgggctg cttccttatg caggaatcgc ataagggaga 661 gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc tccttccggt gggcgcgggg 721 catgactatc gtcgccgcac ttatgactgt tttctttatc atgcaactcg taggacaggt 781 tccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 841 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcacggg 901 ccccgccacc aaacgtttcg gcgagaagca ggccattatc gcgggcatgg cggccgacgc 961 gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg atggccttcc ccattatgat 1021 tcttctcgct tccggcggca tcggtatgcc cgcgttgcag gccatgctgt cccgccaagt 1081 agatgacgac catcagggac agcttcaagg gtcgctcgcg gctcttacca gcctcacttc 1141 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1201 gttggcatgg attgtaggtg ccgcccttta ccttgtctgc ctccccgcgt tgcgtcgcgg 1261 tgcatggagc cgggccacct cgacctaata atactagctc cggcaaaaaa acgggcaagg 1321 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt tgccacctga 1381 cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc acccgtgaag 1441 gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga ctcgaattcg 1501 cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt cttcacaacc 1561 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 1621 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 1681 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 1741 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 1801 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 1861 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 1921 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 1981 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 2041 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 2101 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 2161 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 2221 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 2281 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 2341 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 2401 catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac attgccgtca 2461 ctgcgtcttt tactggcact tctcgctaac caaaccggta accccgctta ttaaaagcat 2521 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca 2581 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt 2641 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc 2701 tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg aagaaGATaa 2761 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 2821 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 2881 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 2941 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 3001 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 3061 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 3121 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagAAAacca tgggctggga 3181 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 3241 gctgaagctg aaggacggcg gccactaTGG Atgcatctcg ggagatagtt tgatcagctt 3301 ggcgagcaca gggaaaagag ttcctattaa ggatttgtta ggcgaaaaag attttgaaat 3361 atgggcaatt aatgaacaga cgatgaagct ggaatcagct aaagttagtc gtgtattttg 3421 taccggcaaa aagctagtct atactctaaa aactcgacta ggtagaacta tcaaggcaac 3481 agcaaatcat agatttttaa ctattgatgg ttggaaaaga ttagatgagc tatctttaaa 3541 agagcatatt gctctacccc gtaaactaga aTAATACTAG AGttgagaag agaaaagaaa 3601 accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc ggtcttaagt 3661 tttttggctg aaTACTAGAG cctttggtcg AAAAAAAAAG CCCGCACTGT CAGGTGCGGG 3721 CTTTTTTctg tgtttccTAC TAGAGCAAGC GAGCTCGATA TCAAATTACG CCCCGCCCTG 3781 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA 3841 AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA 3901 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA 3961 ACTGGTGAAA CTCACCCAGG GATTGGCTGA AACGAAAAAC ATATTCTCAA TAAACCCTTT 4021 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA 4081 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG 4141 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC 4201 CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA 4261 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT 4321 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA 4381 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC 4441 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG 4501 AAAGTTGGAA CCTCTTACGT GCCTACTAGA GGGACCAAAA CGAAAAAAGA CGCTTTTCAG 4561 CGTCTTATTG TTCGTCTTTG GTACCGAGCT CTAGTAGTGA TCTACACTAG CACTATCAGT 4621 GTTATTAATT TTTAAAGTAT GGGCAATCAA TTGCTCCTGT TAAAATTGCT TTAGAAATAC 4681 TTTGGCAGCG GTTTGTTGTA TTGAGTTTCA TTTGCGCATT GGTTAAATGG AAAGTGACAG 4741 TACGCTCACT GCAACCTAAT ATTTTTGAAA TATCCCAAGA GCTTTTTCCT TCGCATGCCC 4801 ACGCTAAACA TTCTTTTTCT CTTTTGGTTA AATCGTTGTT TGATTTATTA TTTGCTATAT 4861 TTATTTTTCG ATAATTATCA ACTAGAGAAG GAACAATTAA TGGTATGTTC ATACACGCAT 4921 GTAAAAATAA ACTATCTATA TAGTTGTCTT TTTCTGAATG TGCAAAACTA AGCATTCCGA 4981 AGCCATTGTT AGCCGTATGA ATAGGGAAAC TAAACCCAGT GATAAGACCT GATGTTTTCG 5041 CTTCTTTAAT TACATTTGGA GATTTTTTAT TTACAGCATT GTTTTCAAAT ATATTCCAAT 5101 TAATTGGTGA ATGATTGGAG TTAGAATAAT CTACTATAGG ATCATATTTT ATTAAATTAG 5161 CGTCATCATA ATATTGCCTC CATTTTTTAG GGTAATTATC TAGGATTGAA ATATCAGATT 5221 TAACCATAGA ATGAGGATAA ATGATCGCGA GTAAATAATA TTCACAATGT ACCATTTTAG 5281 TCATATCAGA TAAGCATTGA TTAATATCAT TATTGCTTCT ACAAGCTTTA ATTTTATTAA 5341 TTATTCTGTA TGTGTCGTCG GCATTTATGT TTTTCATCTA GTATTTCTCC TCTTTAATCT 5401 CTAGTAGCTA GCACAATCCC TAGGACTGAG CTAGCTGTCA ATCACACTGG CTCACCTTCG 5461 GGTGGGCCTT TCTGCGTTTA TATACTAGAC ACCTGTAGGA TCGTACAGGT TTACGCAAGA 5521 AAATGGTTTG TTACTTTCGA ATAAtactag agtcacacag gaaagtacta gATGagctcc 5581 tctttacaat tggcaccaga aatagaaaag ttgcctcaga gtgatattta ctgggacccc 5641 atcgtttcta ttacggagac tggagtcgaa gaggtttttg atttgactgt gccaggacta 5701 cgtaactttg tcgccaatga catcattgta cataacTCAc actacgacgc tgaggtcaag 5761 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag 5821 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag 5881 ggccgccact ccaccggcgg catggacgag ctgtacaagc atcatcacca tcaccactaa 5941 tactagagaa cgcatgagAA AGCCCCCGGA AGATCACCTT CCGGGGGCTT Ttttattgcg 6001 ctactagtag cggccgctgc aggagtcact aagggttagt tagttagatt agcagaaagt 6061 caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc attggggctc 6121 actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgct 6181 agagatggaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6241 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg atctcgcggt 6301 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6361 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6421 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6481 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6541 atcccttaac gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct 6601 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag 6661 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga 6721 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag 6781 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc 6841 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg 6901 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg 6961 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag 7021 gagagcgcac gagggagccg ccaggggaaa cgcctggtat ctttatagtc ctgtcgggtt 7081 tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg 7141 gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc 7201 aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt 7261 agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg ctgacgcacc 7321 ggtgcagcct tttttctcct gccacatgaa gcacttcact gacaccctca tcagtgccaa 7381 catagtaagc cagtatacac tccgctagcg ctgaggtctg cctcgtgaag aaggtgttgc 7441 tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt 7501 gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga 7561 acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt 7621 tattcaacaa agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 7681 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgtt //