LOCUS IBMc391 3668 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321 ACCESSION IBMc391 VERSION IBMc391.1 FEATURES Location/Qualifiers CDS 400..1059 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" primer_bind 3566..3583 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" primer_bind complement(209..225) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" CDS complement(1377..2525) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" terminator 155..249 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="lambda t0 terminator" promoter 302..399 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="cat promoter" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc 121 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg 181 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt 241 ggtgagaatc caggggtccc caataattac gatttaaatt ggcgaaaatg agacgttgat 301 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt 361 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga 421 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca 481 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc 541 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg 601 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt 661 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt 721 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac 781 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc 841 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc 901 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg 961 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa 1021 ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttgacttttg tccttttccg 1081 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg 1141 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc 1201 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg 1261 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg 1321 ccggccgcga tgcaggtggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag 1381 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc 1441 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat 1501 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa 1561 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc 1621 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg 1681 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg 1741 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc 1801 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag 1861 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc 1921 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc 1981 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc 2041 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac 2101 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc 2161 aacgcggcct gcttggcttc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc 2221 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc 2281 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga 2341 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggactat cgagccgacg 2401 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc 2461 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga 2521 ttcattcacc ctccttgcgg gattgccccg gaattaattc cccggatcga tccgtcgatc 2581 ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg 2641 gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata 2701 aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg 2761 cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc 2821 gtttctgcgg actggctttc tacgtggctg ccatttttgg ggtgaggccg ttcgcggccg 2881 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa 2941 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa 3001 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa 3061 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc 3121 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct 3181 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt 3241 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct 3301 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg 3361 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt 3421 tccgcgaggt atccacaacg ccggcggccc tacatggctc tgctgtagtg agtgggttgc 3481 gctccggcag cggtcctgat cccccgcaga aaaaaaggat ctcaagaaga tcctttgatc 3541 ttttctacgg cgcgcccagc tgtctagggc ggcggatttg tcctactcag gagagcgttc 3601 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt 3661 tgatgcct //