LOCUS IBMc393 4406 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB4A3-T-PT7-B30-mScarlet-I-3T ACCESSION IBMc393 VERSION IBMc393.1 FEATURES Location/Qualifiers terminator 3534..3564 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="PTH TERM terminator" promoter 2593..2613 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="PT7" CDS 2643..3338 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="mScarlet-I" RBS 2622..2636 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" rep_origin join(complement(1..1375),complement(3745..4406)) /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="pSC101 origin" CDS join(complement(1..490),complement(3946..4406)) /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="repA" terminator 3397..3525 /ApEinfo_fwdcolor="#ff0000" /ApEinfo_revcolor="#ff0000" /label="T7Tphi terminator" misc_feature 3565..3585 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" primer_bind complement(3719..3738) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" primer_bind 2356..2375 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" terminator 3350..3388 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="BBa_B1006" terminator 2496..2585 /ApEinfo_fwdcolor="#f58a5e" /ApEinfo_revcolor="#f58a5e" /label="ECK120029600" misc_feature 2474..2495 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" ORIGIN 1 ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc 61 tcatagagta tttgttttca aaagacttaa catgttccag attatatttt atgaattttt 121 ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg 181 agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga 241 tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt 301 ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt 361 tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg 421 tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta 481 attcagacat acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg 541 ctagtcaatg ataattacat gtccttttcc tttgagttgt gggtatctgt aaattctgct 601 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt 661 tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa 721 aaaagataaa aagaatagat cccagccctg tgtataactc actactttag tcagttccgc 781 agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc 841 ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct 901 ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg 961 gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg 1021 aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt 1081 ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca 1141 gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa 1201 ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc 1261 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 1321 cattactgga tctatcaaca ggagtccaag cgagctcgta aacttggtct gacagttacc 1381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 1441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 1501 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 1561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 1621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 1681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 1741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 1801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 1861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 1921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 1981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 2041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 2101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 2161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 2221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 2281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 2341 gcacatttcc catggtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 2401 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta 2461 aggatgattt ctggaattcg cggccgcttc tagagttgag aagagaaaag aaaaccgccg 2521 atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg 2581 ctgaaTACTA GAGTAATACG ACTCACTATA GGGTACTAGA GATTAAAGAG GAGAAATACT 2641 AGatggtgag taaaggagaa gctgtgatta aagagttcat gcgcttcaaa gttcacatgg 2701 agggttctat gaacggtcac gagttcgaga tcgaaggcga aggcgagggc cgtccgtatg 2761 aaggcaccca gaccgccaaa ctgaaagtga ctaaaggcgg cccgctgcct ttttcctggg 2821 acatcctgag cccgcaattt atgtacggtt ctagggcgtt catcaaacac ccagcggata 2881 tcccggacta ttataagcag tcttttccgg aaggtttcaa gtgggaacgc gtaatgaatt 2941 ttgaagatgg tggtgccgtg accgtcactc aggacacctc cctggaggat ggcaccctga 3001 tctataaagt taaactgcgt ggtactaatt ttccacctga tggcccggtg atgcagaaaa 3061 agacgatggg ttgggaggcg tctaccgaac gcttgtatcc ggaagatggt gtgctgaaag 3121 gcgacattaa aatggccctg cgcctgaaag atggcggccg ctatctggct gacttcaaaa 3181 ccacgtacaa agccaagaaa cctgtgcaga tgcctggcgc gtacaatgtg gaccgcaaac 3241 tggacatcac ctctcataat gaagattata cggtggtaga gcaatatgag cgctccgagg 3301 gtcgtcattc taccggtggc atggatgaac tatacaaata aTACTAGAGa aaaaaaaacc 3361 ccgcccctga cagggcgggg ttttttttTA CTAGAGctgc taacaaagcc cgaaaggaag 3421 ctgagttggc tgctgccacc gctgagcaat aaCTAGCATA ACCCCTTGGG GCCTCTAAAC 3481 GGGTCTTGAG GGGTTTTTTG ctgaaaggag gaactatatc cggattACTA GAGgtcatgc 3541 ttgccatctg ttttcttgca agattactag tagcggccgc tgcagtccgg caaaaaaggg 3601 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca 3661 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 3721 tcactcaaag gcggtaatct cgaggttaca ttgtcgatct gttcatggtg aacagcttta 3781 aatgcaccaa aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct 3841 gtgcatatgg acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt 3901 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta 3961 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc 4021 attgagatca tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg 4081 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc 4141 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc 4201 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg 4261 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg 4321 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat 4381 gaacttaaat tcatcaaggc taatct //