LOCUS IBMc395 6723 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3K3-TT-PBAD(SapI-)-B30-His-FKBP-14aa-T7RNAP(181+)-T ACCESSION IBMc395 VERSION IBMc395.1 FEATURES Location/Qualifiers RBS 3965..3979 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 6487..6615 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0015" terminator 2641..2693 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="L3S3P21" CDS 4007..4327 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="FKBP E coli optimized" promoter 3673..3956 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" misc_feature 2619..2640 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS 4346..4363 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="linker" primer_bind 2501..2520 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" CDS complement(129..944) /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="kanR" CDS 4364..6478 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="T7 181+" CDS complement(2767..3645) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 6616..6636 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" terminator complement(2702..2758) /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="L3S2P11" rep_origin complement(1276..2100) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" primer_bind complement(47..66) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" CDS 4328..4345 /ApEinfo_fwdcolor="#c6c9d1" /ApEinfo_revcolor="#c6c9d1" /label="linker" CDS 3989..4006 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="H6" ORIGIN 1 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 61 ggtaatctcg agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc 121 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga 181 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg 241 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca 301 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga 361 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca 421 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt 481 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca 541 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa 601 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac 661 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc 721 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt 781 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat 841 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt 901 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta 961 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg 1021 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag 1081 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag 1141 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact 1201 ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga 1261 ggcagacctc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg 1321 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcgtc agcagaatat 1381 gtgatacagg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact 1441 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat 1501 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct 1561 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa 1621 agataccagg cgtttcccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt 1681 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt 1741 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac 1801 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca 1861 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg 1921 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg 1981 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc 2041 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa 2101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 2161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 2221 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 2281 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 2341 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 2401 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 2461 tcctgcaact ttatccgcct ccatccagtc tattccatgg tgccacctga cgtctaagaa 2521 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga 2581 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag 2641 CCAATTATTG AAGGCCTCCC TAACGGGGGG CCTTTTTTTG TTTCTGGTCT CCCTACTAGA 2701 GGGACCAAAA CGAAAAAAGA CGCTCGAAAG CGTCTCTTTT CTGGAATTTG GTACCGAGTA 2761 CTAGAGTTAT GACAACTTGA CGGCTACATC ATTCACTTTT TCTTCACAAC CGGCACGGAA 2821 CTCGCTCGGG CTGGCCCCGG TGCATTTTTT AAATACCCGC GAGAAATAGA GTTGATCGTC 2881 AAAACCAACA TTGCGACCGA CGGTGGCGAT AGGCATCCGG GTGGTGCTCA AAAGCAGCTT 2941 CGCCTGGCTG ATACGTTGGT CCTCGCGCCA GCTTAAGACG CTAATCCCTA ACTGCTGGCG 3001 GAAAAGATGT GACAGACGCG ACGGCGACAA GCAAACATGC TGTGCGACGC TGGCGATATC 3061 AAAATTGCTG TCTGCCAGGT GATCGCTGAT GTACTGACAA GCCTCGCGTA CCCGATTATC 3121 CATCGGTGGA TGGAGCGACT CGTTAATCGC TTCCATGCGC CGCAGTAACA ATTGCTCAAG 3181 CAGATTTATC GCCAGCAGCT CCGAATAGCG CCCTTCCCCT TGCCCGGCGT TAATGATTTG 3241 CCCAAACAGG TCGCTGAAAT GCGGCTGGTG CGCTTCATCC GGGCGAAAGA ACCCCGTATT 3301 GGCAAATATT GACGGCCAGT TAAGCCATTC ATGCCAGTAG GCGCGCGGAC GAAAGTAAAC 3361 CCACTGGTGA TACCATTCGC GAGCCTCCGG ATGACGACCG TAGTGATGAA TCTCTCCTGG 3421 CGGGAACAGC AAAATATCAC CCGGTCGGCA AACAAATTCT CGTCCCTGAT TTTTCACCAC 3481 CCCCTGACCG CGAATGGTGA GATTGAGAAT ATAACCTTTC ATTCCCAGCG GTCGGTCGAT 3541 AAAAAAATCG AGATAACCGT TGGCCTCAAT CGGCGTTAAA CCCGCCACCA GATGGGCATT 3601 AAACGAGTAT CCCGGCAGCA GGGGATCATT TTGCGCTTCA GCCATACTTT TCATACTCCC 3661 GCCATTCAGA GAAGAAACCA ATTGTCCATA TTGCATCAGA CATTGCCGTC ACTGCGTCTT 3721 TTACTGGCAC TTCTCGCTAA CCAAACCGGT AACCCCGCTT ATTAAAAGCA TTCTGTAACA 3781 AAGCGGGACC AAAGCCATGA CAAAAACGCG TAACAAAAGT GTCTATAATC ACGGCAGAAA 3841 AGTCCACATT GATTATTTGC ACGGCGTCAC ACTTTGCTAT GCCATAGCAT TTTTATCCAT 3901 AAGATTAGCG GATCCTACCT GACGCTTTTT ATCGCAACTC TCTACTGTTT CTCCATtact 3961 agagattaaa gaggagaaaT ACTAGATGca tcatcaccat caccacGGAG TTCAGGTAGA 4021 GACAATTAGT CCTGGGGATG GCCGCACATT TCCGAAACGC GGCCAAACCT GTGTGGTACA 4081 CTATACAGGA ATGTTGGAAG ATGGCAAAAA GTTTGATTCC AGTCGTGATC GCAACAAGCC 4141 TTTTAAATTT ATGTTGGGAA AACAGGAGGT AATTCGCGGT TGGGAGGAAG GTGTCGCACA 4201 AATGTCCGTG GGTCAACGCG CCAAGTTGAC GATCTCACCT GATTACGCTT ACGGAGCCAC 4261 CGGCCATCCT GGCATTATTC CCCCACATGC CACTTTGGTC TTTGATGTTG AACTTTTAAA 4321 GTTAGAAACG TCGGGTGGAA GCGGTACGTC GGGTGGAAGC GGTaaagcat ttatgcaagt 4381 tgtcgaggct gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca 4441 taaggaagac tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg 4501 aatggttagc ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga 4561 actcgcacct gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc 4621 tccgatgttc caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg 4681 ctattgggct aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact 4741 gatgcgctac gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa 4801 caccgcatgg aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa 4861 gcattgtccg gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga 4921 agacatcgac atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta 4981 ccgcaaggac aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc 5041 caataagttt gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg 5101 tgtttacgct gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac 5161 gctggcgaaa ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc 5221 aaactgtgcg ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa 5281 ccacgagaac atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca 5341 agattctccg ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg 5401 cctgagctat aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca 5461 cttctccgcg atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga 5521 aaccgttcag gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga 5581 cgcaatcaat gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat 5641 ctctgagaaa gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt 5701 tactcgcagt gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg 5761 cttccgtcaa caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct 5821 gatgttcact cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt 5881 gagcgtgacg gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct 5941 gctggctgct gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt 6001 gcattgggta actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac 6061 gcgcttgaac ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa 6121 agatagcgag attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag 6181 ccaagacggt agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga 6241 atcttttgca ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt 6301 caaagcagtg cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt 6361 ctacgaccag ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc 6421 ggctaaaggt aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaaTA 6481 CTAGAGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA 6541 TCTGTTGTTT GTCGGTGAAC GCTCTCTACT AGAGTCACAC TGGCTCACCT TCGGGTGGGC 6601 CTTTCTGCGT TTATAtacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc 6661 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc 6721 gct //