LOCUS IBMc396 5906 bp ds-DNA circular 18-NOV-2020 DEFINITION pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T ACCESSION IBMc396 VERSION IBMc396.1 FEATURES Location/Qualifiers CDS complement(3615..4763) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="trfA CDS (replication protein)" terminator 2265..2317 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="ECK120033736" CDS 1897..2253 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS 1318..1851 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T " primer_bind complement(2447..2463) /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS2 Rev" promoter 984..1267 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 2638..3297 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="cat" misc_feature 56..77 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" primer_bind 5804..5821 /ApEinfo_fwdcolor="#85dae9" /ApEinfo_revcolor="#85dae9" /label="pSEVA PS1 Fwd" CDS complement(78..956) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" misc_feature 2318..2338 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 1852..1896 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="3x(GGGGS) linker" RBS 1276..1290 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" CDS 1300..1317 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="H6" ORIGIN 1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt 61 cgcggccgct tctagagtta tgacaacttg acggctacat cattcacttt ttcttcacaa 121 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 181 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 241 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 301 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 361 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 421 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 481 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 541 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 601 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 661 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 721 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 781 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 841 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 901 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 961 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 1021 cactgcgtct tttactggcA cttctcgcta accaaaccgg taaccccgct tattaaaagc 1081 attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 1141 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 1201 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 1261 tctccattac tagagattaa agaggagaaa TACTAGATGc atcatcacca tcaccacaac 1321 acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat cccgttcaac 1381 actctggctg accattacgg tgagcgtTCA gctcgcGGAc agttggccct tgagcatgag 1441 tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact taaagctggt 1501 gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc taagatgatt 1561 GCCcgcatca acgactggtt tgaggaagtg aaagctaagc gcggcAGGcg cccgacagcc 1621 ttccagttcC TAAAAgaaat caagccggaa gccgtagcgt acatcaccat taagaccTCT 1681 ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag cgcaatcggt 1741 cggACCattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc taagcacttc 1801 aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa gGGTGGTGGC 1861 GGTAGCGGAG GCGGTGGTAG CGGTGGTGGC GGTAGCGAAG TTCAGCTGCA AGCAAGCGGT 1921 GGTGGTCTGG TTCAGGCAGG CGGTAGCCTG CGTCTGAGCT GTACCGCAAG CGGTCGTACC 1981 GGCACCATTT ATAGCATGGC ATGGTTTCGT CAGGCACCGG GTAAAGAACG TGAATTTCTG 2041 GCAACCGTTG GTTGGAGCAG CGGTATTACC TATTATATGG ATAGCGTGAA AGGTCGCTTT 2101 ACCATTAGCC GTGATAATGC AAAAAATAGC GCCTACCTGC AAATGAATAG CCTGAAACCG 2161 GAAGATACCG CAGTGTATTA TTGTACAGCA ACCCGTGCAT ATAGCGTGGG TTATGATTAT 2221 TGGGGTCAGG GCACCCAGGT TACCGTTAGC CATtaatact agagaacgca tgagAAAGCC 2281 CCCGGAAGAT CACCTTCCGG GGGCTTTttt attgcgctac tagtagcggc cgctgcaggc 2341 atgcaagctt gcggccgcgt cgtgactggg aaaaccctgg cgactagtct tggactcctg 2401 ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc 2461 cgccgggcgt tttttattgg tgagaatcca ggggtcccca ataattacga tttaaattgg 2521 cgaaaatgag acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga 2581 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg 2641 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat 2701 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt 2761 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac 2821 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag 2881 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg 2941 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg 3001 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat 3061 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc 3121 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac 3181 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc 3241 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt 3301 gacttttgtc cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg 3361 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc 3421 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg 3481 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc 3541 tgcgaggctg gccgtaggcc ggccgcgatg caggtggctg ctgaaccccc agccggaact 3601 gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc 3661 accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc 3721 acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc 3781 tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac 3841 ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg 3901 atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc 3961 agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt 4021 aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg 4081 tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg 4141 tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg 4201 taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc 4261 gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct 4321 cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc 4381 tgcttcgtgt gtttcagcaa cgcggcctgc ttggcttcgc tgacctgttt tgccaggtcc 4441 tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac 4501 ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc 4561 gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc 4621 tggactatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt 4681 gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc 4741 ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgga attaattccc 4801 cggatcgatc cgtcgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca 4861 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg 4921 gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag 4981 ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat 5041 tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtggctgcc atttttgggg 5101 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg 5161 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg 5221 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca 5281 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg 5341 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt 5401 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg 5461 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt 5521 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca 5581 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg 5641 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggcccta catggctctg 5701 ctgtagtgag tgggttgcgc tccggcagcg gtcctgatcc cccgcagaaa aaaaggatct 5761 caagaagatc ctttgatctt ttctacggcg cgcccagctg tctagggcgg cggatttgtc 5821 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac 5881 tgagcctttc gttttatttg atgcct //