LOCUS IBMc397 6768 bp ds-DNA circular 18-NOV-2020 DEFINITION pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T ACCESSION IBMc397 VERSION IBMc397.1 FEATURES Location/Qualifiers terminator 1273..1325 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="L3S3P21" misc_feature 5293..5313 /ApEinfo_fwdcolor="#9eafd2" /ApEinfo_revcolor="#9eafd2" /label="RFC10 Suffix" CDS 2621..2638 /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="H6" promoter 2305..2588 /ApEinfo_fwdcolor="#faac61" /ApEinfo_revcolor="#faac61" /label="PBAD(SapI-)" CDS 3041..5155 /ApEinfo_fwdcolor="#b1ff67" /ApEinfo_revcolor="#b1ff67" /label="T7 181+" primer_bind 1133..1152 /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="VF2" primer_bind complement(5447..5466) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="VR" misc_feature 1251..1272 /ApEinfo_fwdcolor="#d6b295" /ApEinfo_revcolor="#d6b295" /label="RFC10 Prefix (full)" CDS complement(1399..2277) /ApEinfo_fwdcolor="#75c6a9" /ApEinfo_revcolor="#75c6a9" /label="araC" CDS 2996..3040 /ApEinfo_fwdcolor="#ff9ccd" /ApEinfo_revcolor="#ff9ccd" /label="3x(GGGGS) linker" RBS 2597..2611 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0030" terminator 5164..5292 /ApEinfo_fwdcolor="#c7b0e3" /ApEinfo_revcolor="#c7b0e3" /label="BBa_B0015" CDS 2639..2995 /ApEinfo_fwdcolor="#d59687" /ApEinfo_revcolor="#d59687" /label="acVHH" CDS complement(5529..6344) /ApEinfo_fwdcolor="#b7e6d7" /ApEinfo_revcolor="#b7e6d7" /label="kanR" rep_origin join(complement(1..732),complement(6676..6768)) /ApEinfo_fwdcolor="#b4abac" /ApEinfo_revcolor="#b4abac" /label="p15A origin of replication" terminator complement(1334..1390) /ApEinfo_fwdcolor="#f8d3a9" /ApEinfo_revcolor="#f8d3a9" /label="L3S2P11" ORIGIN 1 tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc 61 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga 121 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg 181 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg 241 acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc tctcctgttc 301 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc 361 ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc 421 gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga 481 catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa 541 gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag 601 ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa 661 ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga 721 tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 1021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 1081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattccat ggtgccacct 1141 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 1201 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg 1261 ccgcttctag agCCAATTAT TGAAGGCCTC CCTAACGGGG GGCCTTTTTT TGTTTCTGGT 1321 CTCCCTACTA GAGGGACCAA AACGAAAAAA GACGCTCGAA AGCGTCTCTT TTCTGGAATT 1381 TGGTACCGAG TACTAGAGTT ATGACAACTT GACGGCTACA TCATTCACTT TTTCTTCACA 1441 ACCGGCACGG AACTCGCTCG GGCTGGCCCC GGTGCATTTT TTAAATACCC GCGAGAAATA 1501 GAGTTGATCG TCAAAACCAA CATTGCGACC GACGGTGGCG ATAGGCATCC GGGTGGTGCT 1561 CAAAAGCAGC TTCGCCTGGC TGATACGTTG GTCCTCGCGC CAGCTTAAGA CGCTAATCCC 1621 TAACTGCTGG CGGAAAAGAT GTGACAGACG CGACGGCGAC AAGCAAACAT GCTGTGCGAC 1681 GCTGGCGATA TCAAAATTGC TGTCTGCCAG GTGATCGCTG ATGTACTGAC AAGCCTCGCG 1741 TACCCGATTA TCCATCGGTG GATGGAGCGA CTCGTTAATC GCTTCCATGC GCCGCAGTAA 1801 CAATTGCTCA AGCAGATTTA TCGCCAGCAG CTCCGAATAG CGCCCTTCCC CTTGCCCGGC 1861 GTTAATGATT TGCCCAAACA GGTCGCTGAA ATGCGGCTGG TGCGCTTCAT CCGGGCGAAA 1921 GAACCCCGTA TTGGCAAATA TTGACGGCCA GTTAAGCCAT TCATGCCAGT AGGCGCGCGG 1981 ACGAAAGTAA ACCCACTGGT GATACCATTC GCGAGCCTCC GGATGACGAC CGTAGTGATG 2041 AATCTCTCCT GGCGGGAACA GCAAAATATC ACCCGGTCGG CAAACAAATT CTCGTCCCTG 2101 ATTTTTCACC ACCCCCTGAC CGCGAATGGT GAGATTGAGA ATATAACCTT TCATTCCCAG 2161 CGGTCGGTCG ATAAAAAAAT CGAGATAACC GTTGGCCTCA ATCGGCGTTA AACCCGCCAC 2221 CAGATGGGCA TTAAACGAGT ATCCCGGCAG CAGGGGATCA TTTTGCGCTT CAGCCATACT 2281 TTTCATACTC CCGCCATTCA GAGAAGAAAC CAATTGTCCA TATTGCATCA GACATTGCCG 2341 TCACTGCGTC TTTTACTGGC ACTTCTCGCT AACCAAACCG GTAACCCCGC TTATTAAAAG 2401 CATTCTGTAA CAAAGCGGGA CCAAAGCCAT GACAAAAACG CGTAACAAAA GTGTCTATAA 2461 TCACGGCAGA AAAGTCCACA TTGATTATTT GCACGGCGTC ACACTTTGCT ATGCCATAGC 2521 ATTTTTATCC ATAAGATTAG CGGATCCTAC CTGACGCTTT TTATCGCAAC TCTCTACTGT 2581 TTCTCCATta ctagagatta aagaggagaa aTACTAGATG catcatcacc atcaccacGA 2641 AGTTCAGCTG CAAGCAAGCG GTGGTGGTCT GGTTCAGGCA GGCGGTAGCC TGCGTCTGAG 2701 CTGTACCGCA AGCGGTCGTA CCGGCACCAT TTATAGCATG GCATGGTTTC GTCAGGCACC 2761 GGGTAAAGAA CGTGAATTTC TGGCAACCGT TGGTTGGAGC AGCGGTATTA CCTATTATAT 2821 GGATAGCGTG AAAGGTCGCT TTACCATTAG CCGTGATAAT GCAAAAAATA GCGCCTACCT 2881 GCAAATGAAT AGCCTGAAAC CGGAAGATAC CGCAGTGTAT TATTGTACAG CAACCCGTGC 2941 ATATAGCGTG GGTTATGATT ATTGGGGTCA GGGCACCCAG GTTACCGTTA GCCATGGTGG 3001 AGGTGGTTCA GGTGGCGGAG GTTCTGGTGG AGGTGGTTCA aaagcattta tgcaagttgt 3061 cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt cgtggcataa 3121 ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt caaccggaat 3181 ggttagctta caccgccaaa atgctggcgt agtaggtcaa gactctgaga ctatcgaact 3241 cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt gcgctggctg gcatctctcc 3301 gatgttccaa ccttgcgtag ttcctcctaa gccgtggact ggcattactg gtggtggcta 3361 ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact cacagtaaga aagcactgat 3421 gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg attaacattg cgcaaaacac 3481 cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac gtaatcacca agtggaagca 3541 ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa ctcccgatga aaccggaaga 3601 catcgacatg aatcctgagg ctctcaccgc gtggaaacgt gctgccgctg ctgtgtaccg 3661 caaggacaag gctcgcaagt ctcgccgtat cagccttgag ttcatgcttg agcaagccaa 3721 taagtttgct aaccataagg ccatctggtt cccttacaac atggactggc gcggtcgtgt 3781 ttacgctgtg tcaatgttca acccgcaagg taacgatatg accaaaggac tgcttacgct 3841 ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg ctgaaaatcc acggtgcaaa 3901 ctgtgcgggt gtcgataagg ttccgttccc tgagcgcatc aagttcattg aggaaaacca 3961 cgagaacatc atggcttgcg ctaagtctcc actggagaac acttggtggg ctgagcaaga 4021 ttctccgttc tgcttccttg cgttctgctt tgagtacgct ggggtacagc accacggcct 4081 gagctataac tgctcccttc cgctggcgtt tgacgggtct tgctctggca tccagcactt 4141 ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt aacttgcttc ctagtgaaac 4201 cgttcaggac atctacggga ttgttgctaa gaaagtcaac gagattctac aagcagacgc 4261 aatcaatggg accgataacg aagtagttac cgtgaccgat gagaacactg gtgaaatctc 4321 tgagaaagtc aagctgggca ctaaggcact ggctggtcaa tggctggctt acggtgttac 4381 tcgcagtgtg actaagcgtt cagtcatgac gctggcttac gggtccaaag agttcggctt 4441 ccgtcaacaa gtgctggaag ataccattca gccagctatt gattccggca agggtctgat 4501 gttcactcag ccgaatcagg ctgctggata catggctaag ctgatttggg aatctgtgag 4561 cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt aagtctgctg ctaagctgct 4621 ggctgctgag gtcaaagata agaagactgg agagattctt cgcaagcgtt gcgctgtgca 4681 ttgggtaact cctgatggtt tccctgtgtg gcaggaatac aagaagccta ttcagacgcg 4741 cttgaacctg atgttcctcg gtcagttccg cttacagcct accattaaca ccaacaaaga 4801 tagcgagatt gatgcacaca aacaggagtc tggtatcgct cctaactttg tacacagcca 4861 agacggtagc caccttcgta agactgtagt gtgggcacac gagaagtacg gaatcgaatc 4921 ttttgcactg attcacgact ccttcggtac cattccggct gacgctgcga acctgttcaa 4981 agcagtgcgc gaaactatgg ttgacacata tgagtcttgt gatgtactgg ctgatttcta 5041 cgaccagttc gctgaccagt tgcacgagtc tcaattggac aaaatgccag cacttccggc 5101 taaaggtaac ttgaacctcc gtgacatctt agagtcggac ttcgcgttcg cgtaaTACTA 5161 GAGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT 5221 GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG CTCACCTTCG GGTGGGCCTT 5281 TCTGCGTTTA TAtactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc 5341 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct 5401 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 5461 ggtaatctcg agtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc 5521 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga 5581 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg 5641 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca 5701 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga 5761 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca 5821 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt 5881 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca 5941 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa 6001 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac 6061 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc 6121 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt 6181 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat 6241 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt 6301 aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta 6361 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg 6421 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag 6481 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag 6541 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact 6601 ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga 6661 ggcagacctc agcgctagcg gagtgtatac tggcttacta tgttggcact gatgagggtg 6721 tcagtgaagt gcttcatgtg gcaggagaaa aaaggctgca ccggtgcg //